Restriction map for
pF1KB8219
(Linear) (Six-base) MAPSORT of: KIBB8219.seq Check: 3100 from: 1 to: 951
KIBB8219.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 561 951
Size: 561 390
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 951
Size: 623 328
AflII C'TTAA_G
Cuts at: 0 43 951
Size: 43 908
AflIII A'CryG_T
Cuts at: 0 299 951
Size: 299 652
AlwNI CAG_nnn'CTG
Cuts at: 0 328 674 951
Size: 328 346 277
Fragments arranged by size:
346 328 277
ApaI G_GGCC'C
Cuts at: 0 395 951
Size: 395 556
AvaI C'yCGr_G
Cuts at: 0 388 394 951
Size: 388 6 557
Fragments arranged by size:
557 388 6
BanI G'GyrC_C
Cuts at: 0 200 318 340 448 951
Size: 200 118 22 108 503
Fragments arranged by size:
503 200 118 108 22
BanII G_rGCy'C
Cuts at: 0 109 395 951
Size: 109 286 556
Fragments arranged by size:
556 286 109
BbsI GAAGACnn'nnnn_
Cuts at: 0 17 406 951
Size: 17 389 545
Fragments arranged by size:
545 389 17
BclI T'GATC_A
Cuts at: 0 479 951
Size: 479 472
BglI GCCn_nnn'nGGC
Cuts at: 0 805 826 841 951
Size: 805 21 15 110
Fragments arranged by size:
805 110 21 15
Bme1580I G_kGCm'C
Cuts at: 0 203 395 451 951
Size: 203 192 56 500
Fragments arranged by size:
500 203 192 56
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 420 951
Size: 420 531
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 426 509 667 951
Size: 244 182 83 158 284
Fragments arranged by size:
284 244 182 158 83
Bpu10I CC'TnA_GC
Cuts at: 0 887 951
Size: 887 64
BsaI GGTCTCn'nnnn_
Cuts at: 0 391 946 951
Size: 391 555 5
Fragments arranged by size:
555 391 5
BsaBI GATnn'nnATC
Cuts at: 0 68 951
Size: 68 883
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 482 512 846 876 951
Size: 482 30 334 30 75
Fragments arranged by size:
482 334 75 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 928 951
Size: 928 23
BseYI C'CCAG_C
Cuts at: 0 216 882 951
Size: 216 666 69
Fragments arranged by size:
666 216 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 951
Size: 493 458
BsmI GAATG_Cn'
Cuts at: 0 633 951
Size: 633 318
BsmBI CGTCTCn'nnnn_
Cuts at: 0 754 951
Size: 754 197
Bsp1286I G_dGCh'C
Cuts at: 0 109 203 395 451 951
Size: 109 94 192 56 500
Fragments arranged by size:
500 192 109 94 56
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 561 951
Size: 561 390
BsrDI GCAATG_nn'
Cuts at: 0 344 666 951
Size: 344 322 285
Fragments arranged by size:
344 322 285
BstXI CCAn_nnnn'nTGG
Cuts at: 0 382 951
Size: 382 569
BstYI r'GATC_y
Cuts at: 0 125 951
Size: 125 826
DrdI GACnn_nn'nnGTC
Cuts at: 0 27 951
Size: 27 924
EaeI y'GGCC_r
Cuts at: 0 308 951
Size: 308 643
EarI CTCTTCn'nnn_
Cuts at: 0 899 951
Size: 899 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 426 509 623 667 951
Size: 244 182 83 114 44 284
Fragments arranged by size:
284 244 182 114 83 44
EcoO109I rG'GnC_Cy
Cuts at: 0 279 951
Size: 279 672
EcoRV GAT'ATC
Cuts at: 0 615 951
Size: 615 336
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 423 819 951
Size: 423 396 132
Fragments arranged by size:
423 396 132
FspI TGC'GCA
Cuts at: 0 184 951
Size: 184 767
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 926 951
Size: 926 25
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 78 890 899 951
Size: 56 22 812 9 52
Fragments arranged by size:
812 56 52 22 9
MscI TGG'CCA
Cuts at: 0 310 951
Size: 310 641
MslI CAynn'nnrTG
Cuts at: 0 178 951
Size: 178 773
MspA1I CmG'CkG
Cuts at: 0 325 477 859 951
Size: 325 152 382 92
Fragments arranged by size:
382 325 152 92
NspI r_CATG'y
Cuts at: 0 135 303 531 951
Size: 135 168 228 420
Fragments arranged by size:
420 228 168 135
PasI CC'CwG_GG
Cuts at: 0 5 951
Size: 5 946
PciI A'CATG_T
Cuts at: 0 299 951
Size: 299 652
PflMI CCAn_nnn'nTGG
Cuts at: 0 60 951
Size: 60 891
PfoI T'CCnGG_A
Cuts at: 0 644 951
Size: 644 307
PpuMI rG'GwC_Cy
Cuts at: 0 279 951
Size: 279 672
PspOMI G'GGCC_C
Cuts at: 0 391 951
Size: 391 560
PstI C_TGCA'G
Cuts at: 0 683 879 951
Size: 683 196 72
Fragments arranged by size:
683 196 72
PvuII CAG'CTG
Cuts at: 0 325 477 859 951
Size: 325 152 382 92
Fragments arranged by size:
382 325 152 92
SfcI C'TryA_G
Cuts at: 0 679 875 951
Size: 679 196 76
Fragments arranged by size:
679 196 76
SmlI C'TyrA_G
Cuts at: 0 43 951
Size: 43 908
StuI AGG'CCT
Cuts at: 0 799 951
Size: 799 152
StyI C'CwwG_G
Cuts at: 0 272 899 951
Size: 272 627 52
Fragments arranged by size:
627 272 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 482 514 846 878 951
Size: 482 32 332 32 73
Fragments arranged by size:
482 332 73 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 79 951
Size: 79 872
Enzymes that do cut:
AarI AcuI AflII AflIII AlwNI ApaI AvaI BanI
BanII BbsI BclI BglI Bme1580I BplI BpmI Bpu10I
BsaI BsaBI BsaXI BseRI BseYI BsgI BsmI BsmBI
Bsp1286I BspMI BsrDI BstXI BstYI DrdI EaeI EarI
Eco57MI EcoO109I EcoRV FalI FspI Hin4I MmeI MscI
MslI MspA1I NspI PasI PciI PflMI PfoI PpuMI
PspOMI PstI PvuII SfcI SmlI StuI StyI TstI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglII
BlpI BmgBI BmrI BmtI BpuEI BsaAI BsaHI BsaWI
BsiEI BsiHKAI BsiWI BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII EagI EciI
EcoICRI EcoNI EcoRI FseI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TatI Tth111I XbaI XcmI
XhoI XmaI ZraI