(Linear) (Six-base) MAPSORT of: KIBB8213.seq  Check: 6976  from: 1  to: 462



KIBB8213.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     140     462 
   Size:         46      94     322

  Fragments arranged by size: 

                 322      94      46
 
AleI CACnn'nnGTG

Cuts at:      0     352     462 
   Size:        352     110
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     125     157     462 
   Size:        125      32     305

  Fragments arranged by size: 

                 305     125      32
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      34     462 
   Size:         34     428
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     320     462 
   Size:        320     142
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     200     462 
   Size:         64     136     262

  Fragments arranged by size: 

                 262     136      64
 
BspHI T'CATG_A

Cuts at:      0     144     462 
   Size:        144     318
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     172     462 
   Size:        172     290
 
BsrDI GCAATG_nn'

Cuts at:      0     264     330     462 
   Size:        264      66     132

  Fragments arranged by size: 

                 264     132      66
 
EaeI y'GGCC_r

Cuts at:      0     284     342     462 
   Size:        284      58     120

  Fragments arranged by size: 

                 284     120      58
 
EarI CTCTTCn'nnn_

Cuts at:      0     227     462 
   Size:        227     235
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     140     462 
   Size:         46      94     322

  Fragments arranged by size: 

                 322      94      46
 
EcoO109I rG'GnC_Cy

Cuts at:      0     184     462 
   Size:        184     278
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     225     462 
   Size:        225     237
 
MslI CAynn'nnrTG

Cuts at:      0     143     352     462 
   Size:        143     209     110

  Fragments arranged by size: 

                 209     143     110
 
NspI r_CATG'y

Cuts at:      0     244     462 
   Size:        244     218
 
PpuMI rG'GwC_Cy

Cuts at:      0     184     462 
   Size:        184     278
 
StuI AGG'CCT

Cuts at:      0     125     462 
   Size:        125     337
 
TatI w'GTAC_w

Cuts at:      0     403     462 
   Size:        403      59
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     341     373     462 
   Size:        341      32      89

  Fragments arranged by size: 

                 341      89      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      92     337     462 
   Size:         92     245     125

  Fragments arranged by size: 

                 245     125      92
 

 Enzymes that do cut:

     AcuI     AleI     AloI     BmrI     BsaI     BsgI    BspHI    BspMI
    BsrDI     EaeI     EarI  Eco57MI EcoO109I     MmeI     MslI     NspI
    PpuMI     StuI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
    TaqII  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI