Restriction map for
pF1KB8182
(Linear) (Six-base) MAPSORT of: KIBB8182.seq Check: 7929 from: 1 to: 696
KIBB8182.orf
With 182 enzymes: SgfI *
June 16, 2009 11:53 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 571 696
Size: 536 35 125
Fragments arranged by size:
536 125 35
AleI CACnn'nnGTG
Cuts at: 0 64 696
Size: 64 632
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 517 549 696
Size: 517 32 147
Fragments arranged by size:
517 147 32
AlwNI CAG_nnn'CTG
Cuts at: 0 172 696
Size: 172 524
ApoI r'AATT_y
Cuts at: 0 581 696
Size: 581 115
BanI G'GyrC_C
Cuts at: 0 160 696
Size: 160 536
BanII G_rGCy'C
Cuts at: 0 472 696
Size: 472 224
BbsI GAAGACnn'nnnn_
Cuts at: 0 383 696
Size: 383 313
BbvCI CC'TCA_GC
Cuts at: 0 200 696
Size: 200 496
BfrBI ATG'CAT
Cuts at: 0 430 696
Size: 430 266
BmrI ACTGGGnnnn_n'
Cuts at: 0 636 696
Size: 636 60
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 486 696
Size: 486 210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 696
Size: 478 218
Bpu10I CC'TnA_GC
Cuts at: 0 200 353 696
Size: 200 153 343
Fragments arranged by size:
343 200 153
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 217 355 475 480 696
Size: 217 138 120 5 216
Fragments arranged by size:
217 216 138 120 5
BsiHKAI G_wGCw'C
Cuts at: 0 472 696
Size: 472 224
BsmI GAATG_Cn'
Cuts at: 0 430 696
Size: 430 266
Bsp1286I G_dGCh'C
Cuts at: 0 472 696
Size: 472 224
BsrBI CCG'CTC
Cuts at: 0 631 696
Size: 631 65
BstBI TT'CG_AA
Cuts at: 0 130 696
Size: 130 566
BstEII G'GTnAC_C
Cuts at: 0 27 696
Size: 27 669
BstXI CCAn_nnnn'nTGG
Cuts at: 0 270 696
Size: 270 426
BtgI C'CryG_G
Cuts at: 0 62 696
Size: 62 634
EaeI y'GGCC_r
Cuts at: 0 635 696
Size: 635 61
EarI CTCTTCn'nnn_
Cuts at: 0 186 392 512 696
Size: 186 206 120 184
Fragments arranged by size:
206 186 184 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 55 696
Size: 55 641
EcoICRI GAG'CTC
Cuts at: 0 470 696
Size: 470 226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 536 571 696
Size: 478 58 35 125
Fragments arranged by size:
478 125 58 35
HincII GTy'rAC
Cuts at: 0 305 696
Size: 305 391
HpaI GTT'AAC
Cuts at: 0 305 696
Size: 305 391
MscI TGG'CCA
Cuts at: 0 637 696
Size: 637 59
MslI CAynn'nnrTG
Cuts at: 0 61 64 696
Size: 61 3 632
Fragments arranged by size:
632 61 3
MspA1I CmG'CkG
Cuts at: 0 141 393 447 696
Size: 141 252 54 249
Fragments arranged by size:
252 249 141 54
NsiI A_TGCA'T
Cuts at: 0 432 696
Size: 432 264
NspI r_CATG'y
Cuts at: 0 681 696
Size: 681 15
PstI C_TGCA'G
Cuts at: 0 575 696
Size: 575 121
PvuII CAG'CTG
Cuts at: 0 393 447 696
Size: 393 54 249
Fragments arranged by size:
393 249 54
SacI G_AGCT'C
Cuts at: 0 472 696
Size: 472 224
SfcI C'TryA_G
Cuts at: 0 480 571 696
Size: 480 91 125
Fragments arranged by size:
480 125 91
SmlI C'TyrA_G
Cuts at: 0 682 696
Size: 682 14
SphI G_CATG'C
Cuts at: 0 681 696
Size: 681 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 290 422 622 655 696
Size: 290 132 200 33 41
Fragments arranged by size:
290 200 132 41 33
Enzymes that do cut:
AcuI AleI AloI AlwNI ApoI BanI BanII BbsI
BbvCI BfrBI BmrI BplI BpmI Bpu10I BseRI BsiHKAI
BsmI Bsp1286I BsrBI BstBI BstEII BstXI BtgI EaeI
EarI EciI EcoICRI Eco57MI HincII HpaI MscI MslI
MspA1I NsiI NspI PstI PvuII SacI SfcI SmlI
SphI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmtI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HindIII KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI StyI
SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI