Restriction map for
pF1KB8180
(Linear) (Six-base) MAPSORT of: KIBB8180.seq Check: 2437 from: 1 to: 1275
KIBB8180.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AccI GT'mk_AC
Cuts at: 0 1007 1275
Size: 1007 268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 1177 1275
Size: 875 302 98
Fragments arranged by size:
875 302 98
AfeI AGC'GCT
Cuts at: 0 1053 1275
Size: 1053 222
AflII C'TTAA_G
Cuts at: 0 958 1275
Size: 958 317
AleI CACnn'nnGTG
Cuts at: 0 1264 1275
Size: 1264 11
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 577 580 887 1275
Size: 577 3 307 388
Fragments arranged by size:
577 388 307 3
AlwNI CAG_nnn'CTG
Cuts at: 0 813 1275
Size: 813 462
ApaI G_GGCC'C
Cuts at: 0 483 840 1275
Size: 483 357 435
Fragments arranged by size:
483 435 357
ApaLI G'TGCA_C
Cuts at: 0 872 1275
Size: 872 403
ApoI r'AATT_y
Cuts at: 0 1090 1275
Size: 1090 185
AvaI C'yCGr_G
Cuts at: 0 833 1275
Size: 833 442
BanI G'GyrC_C
Cuts at: 0 528 745 1275
Size: 528 217 530
Fragments arranged by size:
530 528 217
BanII G_rGCy'C
Cuts at: 0 8 27 483 827 840 1275
Size: 8 19 456 344 13 435
Fragments arranged by size:
456 435 344 19 13 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 889 909 1275
Size: 889 20 366
Fragments arranged by size:
889 366 20
BbvCI CC'TCA_GC
Cuts at: 0 9 753 1275
Size: 9 744 522
Fragments arranged by size:
744 522 9
BglI GCCn_nnn'nGGC
Cuts at: 0 160 478 850 1133 1275
Size: 160 318 372 283 142
Fragments arranged by size:
372 318 283 160 142
BglII A'GATC_T
Cuts at: 0 638 1275
Size: 638 637
Bme1580I G_kGCm'C
Cuts at: 0 483 533 840 876 1275
Size: 483 50 307 36 399
Fragments arranged by size:
483 399 307 50 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 691 1275
Size: 691 584
BmtI G_CTAG'C
Cuts at: 0 577 1275
Size: 577 698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 823 1275
Size: 56 767 452
Fragments arranged by size:
767 452 56
Bpu10I CC'TnA_GC
Cuts at: 0 9 569 753 1275
Size: 9 560 184 522
Fragments arranged by size:
560 522 184 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1167 1275
Size: 1167 108
BseYI C'CCAG_C
Cuts at: 0 614 792 827 1275
Size: 614 178 35 448
Fragments arranged by size:
614 448 178 35
BsiHKAI G_wGCw'C
Cuts at: 0 469 876 1250 1275
Size: 469 407 374 25
Fragments arranged by size:
469 407 374 25
BsmI GAATG_Cn'
Cuts at: 0 556 1275
Size: 556 719
Bsp1286I G_dGCh'C
Cuts at: 0 8 27 469 483 533 827 840 876
Size: 8 19 442 14 50 294 13 36
Cuts at: 876 1250 1275
Size: 374 25
Fragments arranged by size:
442 374 294 50 36 25 19 14
13 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 941 1275
Size: 941 334
BsrBI CCG'CTC
Cuts at: 0 1175 1275
Size: 1175 100
BsrFI r'CCGG_y
Cuts at: 0 300 1275
Size: 300 975
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1245 1275
Size: 1245 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 312 1275
Size: 312 963
BstYI r'GATC_y
Cuts at: 0 638 737 1275
Size: 638 99 538
Fragments arranged by size:
638 538 99
BtgI C'CryG_G
Cuts at: 0 483 695 1275
Size: 483 212 580
Fragments arranged by size:
580 483 212
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 132 402 1275
Size: 132 270 873
Fragments arranged by size:
873 270 132
BtsI GCAGTG_nn'
Cuts at: 0 404 564 1275
Size: 404 160 711
Fragments arranged by size:
711 404 160
EaeI y'GGCC_r
Cuts at: 0 206 302 342 518 692 1275
Size: 206 96 40 176 174 583
Fragments arranged by size:
583 206 176 174 96 40
EarI CTCTTCn'nnn_
Cuts at: 0 598 1275
Size: 598 677
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 231 970 1275
Size: 231 739 305
Fragments arranged by size:
739 305 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 823 875 1177 1275
Size: 56 767 52 302 98
Fragments arranged by size:
767 302 98 56 52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 355 805 1275
Size: 355 450 470
Fragments arranged by size:
470 450 355
EcoO109I rG'GnC_Cy
Cuts at: 0 36 141 798 810 1141 1161 1275
Size: 36 105 657 12 331 20 114
Fragments arranged by size:
657 331 114 105 36 20 12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1131 1275
Size: 1131 144
HaeII r_GCGC'y
Cuts at: 0 780 1055 1275
Size: 780 275 220
Fragments arranged by size:
780 275 220
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1205 1237 1275
Size: 1205 32 38
Fragments arranged by size:
1205 38 32
HincII GTy'rAC
Cuts at: 0 258 1275
Size: 258 1017
MscI TGG'CCA
Cuts at: 0 520 694 1275
Size: 520 174 581
Fragments arranged by size:
581 520 174
MslI CAynn'nnrTG
Cuts at: 0 526 694 1264 1275
Size: 526 168 570 11
Fragments arranged by size:
570 526 168 11
MspA1I CmG'CkG
Cuts at: 0 431 792 1275
Size: 431 361 483
Fragments arranged by size:
483 431 361
NaeI GCC'GGC
Cuts at: 0 302 1275
Size: 302 973
NcoI C'CATG_G
Cuts at: 0 695 1275
Size: 695 580
NgoMIV G'CCGG_C
Cuts at: 0 300 1275
Size: 300 975
NheI G'CTAG_C
Cuts at: 0 573 1275
Size: 573 702
NspI r_CATG'y
Cuts at: 0 1243 1275
Size: 1243 32
PasI CC'CwG_GG
Cuts at: 0 19 1275
Size: 19 1256
PflMI CCAn_nnn'nTGG
Cuts at: 0 1035 1275
Size: 1035 240
PfoI T'CCnGG_A
Cuts at: 0 1094 1275
Size: 1094 181
PpuMI rG'GwC_Cy
Cuts at: 0 36 141 798 1141 1275
Size: 36 105 657 343 134
Fragments arranged by size:
657 343 134 105 36
PspOMI G'GGCC_C
Cuts at: 0 479 836 1275
Size: 479 357 439
Fragments arranged by size:
479 439 357
PstI C_TGCA'G
Cuts at: 0 543 1275
Size: 543 732
PvuII CAG'CTG
Cuts at: 0 792 1275
Size: 792 483
SanDI GG'GwC_CC
Cuts at: 0 36 1141 1275
Size: 36 1105 134
Fragments arranged by size:
1105 134 36
SapI GCTCTTCn'nnn_
Cuts at: 0 598 1275
Size: 598 677
SfcI C'TryA_G
Cuts at: 0 373 539 1275
Size: 373 166 736
Fragments arranged by size:
736 373 166
SmaI CCC'GGG
Cuts at: 0 835 1275
Size: 835 440
SmlI C'TyrA_G
Cuts at: 0 958 1275
Size: 958 317
StyI C'CwwG_G
Cuts at: 0 695 1275
Size: 695 580
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 464 1275
Size: 464 811
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 312 756 897 1275
Size: 312 444 141 378
Fragments arranged by size:
444 378 312 141
XmaI C'CCGG_G
Cuts at: 0 833 1275
Size: 833 442
Enzymes that do cut:
AccI AcuI AfeI AflII AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbsI BbvCI BglI
BglII Bme1580I BmrI BmtI BpmI Bpu10I BseRI BseYI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI
BstYI BtgI BtgZI BtsI EaeI EarI EciI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII MscI MslI
MspA1I NaeI NcoI NgoMIV NheI NspI PasI PflMI
PfoI PpuMI PspOMI PstI PvuII SanDI SapI SfcI
SmaI SmlI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI AhdI AloI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI
BciVI BclI BfrBI BlpI BmgBI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI NarI NdeI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XhoI XmnI ZraI