Restriction map for
pF1KB8166
(Linear) (Six-base) MAPSORT of: KIBB8166.seq Check: 9380 from: 1 to: 1932
KIBB8166.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1675 1932
Size: 1675 257
AccI GT'mk_AC
Cuts at: 0 1112 1787 1932
Size: 1112 675 145
Fragments arranged by size:
1112 675 145
Acc65I G'GTAC_C
Cuts at: 0 590 1932
Size: 590 1342
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 533 1373 1932
Size: 88 445 840 559
Fragments arranged by size:
840 559 445 88
AflII C'TTAA_G
Cuts at: 0 1198 1932
Size: 1198 734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1683 1894 1932
Size: 1683 211 38
Fragments arranged by size:
1683 211 38
AlwNI CAG_nnn'CTG
Cuts at: 0 1278 1513 1849 1932
Size: 1278 235 336 83
Fragments arranged by size:
1278 336 235 83
ApoI r'AATT_y
Cuts at: 0 301 343 602 1932
Size: 301 42 259 1330
Fragments arranged by size:
1330 301 259 42
AvaI C'yCGr_G
Cuts at: 0 1418 1932
Size: 1418 514
BamHI G'GATC_C
Cuts at: 0 387 1932
Size: 387 1545
BanI G'GyrC_C
Cuts at: 0 590 1773 1932
Size: 590 1183 159
Fragments arranged by size:
1183 590 159
BanII G_rGCy'C
Cuts at: 0 891 1144 1932
Size: 891 253 788
Fragments arranged by size:
891 788 253
BbsI GAAGACnn'nnnn_
Cuts at: 0 38 335 1199 1538 1932
Size: 38 297 864 339 394
Fragments arranged by size:
864 394 339 297 38
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 611 645 1932
Size: 611 34 1287
Fragments arranged by size:
1287 611 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 844 1722 1932
Size: 844 878 210
Fragments arranged by size:
878 844 210
BglI GCCn_nnn'nGGC
Cuts at: 0 840 1932
Size: 840 1092
BglII A'GATC_T
Cuts at: 0 926 1248 1932
Size: 926 322 684
Fragments arranged by size:
926 684 322
BmrI ACTGGGnnnn_n'
Cuts at: 0 873 1932
Size: 873 1059
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 1932
Size: 482 1450
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1609 1932
Size: 1609 323
BsaI GGTCTCn'nnnn_
Cuts at: 0 707 1932
Size: 707 1225
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1751 1781 1932
Size: 1751 30 151
Fragments arranged by size:
1751 151 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 854 1339 1932
Size: 854 485 593
Fragments arranged by size:
854 593 485
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 1900 1932
Size: 431 1469 32
Fragments arranged by size:
1469 431 32
BsiHKAI G_wGCw'C
Cuts at: 0 627 1932
Size: 627 1305
Bsp1286I G_dGCh'C
Cuts at: 0 627 891 1144 1932
Size: 627 264 253 788
Fragments arranged by size:
788 627 264 253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 734 1675 1857 1932
Size: 734 941 182 75
Fragments arranged by size:
941 734 182 75
BsrFI r'CCGG_y
Cuts at: 0 839 1932
Size: 839 1093
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1849 1932
Size: 1849 83
BstEII G'GTnAC_C
Cuts at: 0 476 1932
Size: 476 1456
BstXI CCAn_nnnn'nTGG
Cuts at: 0 681 1932
Size: 681 1251
BstYI r'GATC_y
Cuts at: 0 387 926 1248 1932
Size: 387 539 322 684
Fragments arranged by size:
684 539 387 322
BtgI C'CryG_G
Cuts at: 0 423 1932
Size: 423 1509
BtsI GCAGTG_nn'
Cuts at: 0 627 662 1932
Size: 627 35 1270
Fragments arranged by size:
1270 627 35
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 491 526 1932
Size: 491 35 1406
Fragments arranged by size:
1406 491 35
EaeI y'GGCC_r
Cuts at: 0 1117 1932
Size: 1117 815
EarI CTCTTCn'nnn_
Cuts at: 0 1586 1932
Size: 1586 346
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 858 1932
Size: 858 1074
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 482 533 1373 1932
Size: 88 394 51 840 559
Fragments arranged by size:
840 559 394 88 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 200 1932
Size: 200 1732
EcoO109I rG'GnC_Cy
Cuts at: 0 1846 1932
Size: 1846 86
EcoRI G'AATT_C
Cuts at: 0 343 1932
Size: 343 1589
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 118 771 1630 1932
Size: 118 653 859 302
Fragments arranged by size:
859 653 302 118
HaeII r_GCGC'y
Cuts at: 0 672 1932
Size: 672 1260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 911 943 1932
Size: 911 32 989
Fragments arranged by size:
989 911 32
HindIII A'AGCT_T
Cuts at: 0 16 1373 1932
Size: 16 1357 559
Fragments arranged by size:
1357 559 16
KpnI G_GTAC'C
Cuts at: 0 594 1932
Size: 594 1338
MfeI C'AATT_G
Cuts at: 0 811 1932
Size: 811 1121
MscI TGG'CCA
Cuts at: 0 1119 1932
Size: 1119 813
MslI CAynn'nnrTG
Cuts at: 0 1876 1932
Size: 1876 56
MspA1I CmG'CkG
Cuts at: 0 643 1266 1275 1884 1932
Size: 643 623 9 609 48
Fragments arranged by size:
643 623 609 48 9
NcoI C'CATG_G
Cuts at: 0 423 1932
Size: 423 1509
NdeI CA'TA_TG
Cuts at: 0 221 1932
Size: 221 1711
PflMI CCAn_nnn'nTGG
Cuts at: 0 897 1932
Size: 897 1035
PpuMI rG'GwC_Cy
Cuts at: 0 1846 1932
Size: 1846 86
PshAI GACnn'nnGTC
Cuts at: 0 781 1932
Size: 781 1151
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 37 69 1932
Size: 37 32 1863
Fragments arranged by size:
1863 37 32
PstI C_TGCA'G
Cuts at: 0 690 1283 1932
Size: 690 593 649
Fragments arranged by size:
690 649 593
PvuII CAG'CTG
Cuts at: 0 643 1266 1275 1884 1932
Size: 643 623 9 609 48
Fragments arranged by size:
643 623 609 48 9
ScaI AGT'ACT
Cuts at: 0 46 1932
Size: 46 1886
SexAI A'CCwGG_T
Cuts at: 0 1796 1932
Size: 1796 136
SfcI C'TryA_G
Cuts at: 0 686 1279 1821 1932
Size: 686 593 542 111
Fragments arranged by size:
686 593 542 111
SmlI C'TyrA_G
Cuts at: 0 1198 1418 1624 1932
Size: 1198 220 206 308
Fragments arranged by size:
1198 308 220 206
StuI AGG'CCT
Cuts at: 0 610 1497 1932
Size: 610 887 435
Fragments arranged by size:
887 610 435
StyI C'CwwG_G
Cuts at: 0 423 1319 1492 1932
Size: 423 896 173 440
Fragments arranged by size:
896 440 423 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 771 1932
Size: 771 1161
TatI w'GTAC_w
Cuts at: 0 44 110 1932
Size: 44 66 1822
Fragments arranged by size:
1822 66 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 192 224 617 649 1932
Size: 192 32 393 32 1283
Fragments arranged by size:
1283 393 192 32 32
XhoI C'TCGA_G
Cuts at: 0 1418 1932
Size: 1418 514
XmnI GAAnn'nnTTC
Cuts at: 0 602 1096 1375 1932
Size: 602 494 279 557
Fragments arranged by size:
602 557 494 279
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AlfI AlwNI ApoI
AvaI BamHI BanI BanII BbsI BcgI BciVI BglI
BglII BmrI BpmI BpuEI BsaI BsaXI BseRI BsgI
BsiHKAI Bsp1286I BspMI BsrFI BstAPI BstEII BstXI BstYI
BtgI BtsI CspCI EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HindIII KpnI MfeI
MscI MslI MspA1I NcoI NdeI PflMI PpuMI PshAI
PsrI PstI PvuII ScaI SexAI SfcI SmlI StuI
StyI TaqII TatI TstI XhoI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflIII AgeI AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BclI BfrBI BlpI Bme1580I BmgBI BmtI BplI
Bpu10I BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstZ17I Bsu36I BtgZI ClaI DraI DraIII
DrdI EagI EcoICRI EcoRV FseI FspI FspAI HincII
HpaI KasI MluI MmeI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PsiI PspOMI PspXI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XcmI XmaI ZraI