Restriction map for
pF1KB8160
(Linear) (Six-base) MAPSORT of: KIBB8160.seq Check: 6985 from: 1 to: 780
KIBB8160.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AflIII A'CryG_T
Cuts at: 0 305 780
Size: 305 475
AleI CACnn'nnGTG
Cuts at: 0 412 780
Size: 412 368
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 169 201 780
Size: 169 32 579
Fragments arranged by size:
579 169 32
AlwNI CAG_nnn'CTG
Cuts at: 0 149 423 780
Size: 149 274 357
Fragments arranged by size:
357 274 149
ApoI r'AATT_y
Cuts at: 0 80 183 772 780
Size: 80 103 589 8
Fragments arranged by size:
589 103 80 8
BanI G'GyrC_C
Cuts at: 0 268 648 780
Size: 268 380 132
Fragments arranged by size:
380 268 132
BanII G_rGCy'C
Cuts at: 0 47 143 780
Size: 47 96 637
Fragments arranged by size:
637 96 47
BbsI GAAGACnn'nnnn_
Cuts at: 0 308 780
Size: 308 472
BbvCI CC'TCA_GC
Cuts at: 0 709 780
Size: 709 71
BfrBI ATG'CAT
Cuts at: 0 644 780
Size: 644 136
BglI GCCn_nnn'nGGC
Cuts at: 0 250 780
Size: 250 530
BlpI GC'TnA_GC
Cuts at: 0 142 780
Size: 142 638
Bme1580I G_kGCm'C
Cuts at: 0 473 665 761 780
Size: 473 192 96 19
Fragments arranged by size:
473 192 96 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 750 780
Size: 750 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 500 780
Size: 61 439 280
Fragments arranged by size:
439 280 61
Bpu10I CC'TnA_GC
Cuts at: 0 709 780
Size: 709 71
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 332 780
Size: 332 448
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 436 780
Size: 310 126 344
Fragments arranged by size:
344 310 126
BsiHKAI G_wGCw'C
Cuts at: 0 47 143 780
Size: 47 96 637
Fragments arranged by size:
637 96 47
BsmI GAATG_Cn'
Cuts at: 0 295 646 780
Size: 295 351 134
Fragments arranged by size:
351 295 134
Bsp1286I G_dGCh'C
Cuts at: 0 47 143 473 665 761 780
Size: 47 96 330 192 96 19
Fragments arranged by size:
330 192 96 96 47 19
BspHI T'CATG_A
Cuts at: 0 297 780
Size: 297 483
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 609 780
Size: 609 171
BsrDI GCAATG_nn'
Cuts at: 0 461 473 780
Size: 461 12 307
Fragments arranged by size:
461 307 12
BsrFI r'CCGG_y
Cuts at: 0 651 780
Size: 651 129
BsrGI T'GTAC_A
Cuts at: 0 749 780
Size: 749 31
BssSI C'ACGA_G
Cuts at: 0 664 780
Size: 664 116
BstAPI GCAn_nnn'nTGC
Cuts at: 0 567 780
Size: 567 213
BstBI TT'CG_AA
Cuts at: 0 528 780
Size: 528 252
BstYI r'GATC_y
Cuts at: 0 722 780
Size: 722 58
Bsu36I CC'TnA_GG
Cuts at: 0 360 517 780
Size: 360 157 263
Fragments arranged by size:
360 263 157
BtgI C'CryG_G
Cuts at: 0 53 198 780
Size: 53 145 582
Fragments arranged by size:
582 145 53
EaeI y'GGCC_r
Cuts at: 0 56 780
Size: 56 724
EcoICRI GAG'CTC
Cuts at: 0 45 141 780
Size: 45 96 639
Fragments arranged by size:
639 96 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 500 780
Size: 61 439 280
Fragments arranged by size:
439 280 61
EcoRI G'AATT_C
Cuts at: 0 772 780
Size: 772 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 651 780
Size: 651 129
HaeII r_GCGC'y
Cuts at: 0 149 780
Size: 149 631
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 322 354 780
Size: 27 295 32 426
Fragments arranged by size:
426 295 32 27
MslI CAynn'nnrTG
Cuts at: 0 58 288 412 466 780
Size: 58 230 124 54 314
Fragments arranged by size:
314 230 124 58 54
MspA1I CmG'CkG
Cuts at: 0 39 457 558 780
Size: 39 418 101 222
Fragments arranged by size:
418 222 101 39
NcoI C'CATG_G
Cuts at: 0 53 198 780
Size: 53 145 582
Fragments arranged by size:
582 145 53
NsiI A_TGCA'T
Cuts at: 0 646 780
Size: 646 134
NspI r_CATG'y
Cuts at: 0 309 750 780
Size: 309 441 30
Fragments arranged by size:
441 309 30
PasI CC'CwG_GG
Cuts at: 0 347 780
Size: 347 433
PciI A'CATG_T
Cuts at: 0 305 780
Size: 305 475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 430 462 780
Size: 430 32 318
Fragments arranged by size:
430 318 32
PstI C_TGCA'G
Cuts at: 0 38 551 780
Size: 38 513 229
Fragments arranged by size:
513 229 38
PvuII CAG'CTG
Cuts at: 0 39 780
Size: 39 741
SacI G_AGCT'C
Cuts at: 0 47 143 780
Size: 47 96 637
Fragments arranged by size:
637 96 47
SfcI C'TryA_G
Cuts at: 0 34 547 585 780
Size: 34 513 38 195
Fragments arranged by size:
513 195 38 34
StuI AGG'CCT
Cuts at: 0 358 780
Size: 358 422
StyI C'CwwG_G
Cuts at: 0 53 198 410 780
Size: 53 145 212 370
Fragments arranged by size:
370 212 145 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 211 780
Size: 211 569
TatI w'GTAC_w
Cuts at: 0 749 780
Size: 749 31
XbaI T'CTAG_A
Cuts at: 0 157 780
Size: 157 623
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 282 780
Size: 282 498
Enzymes that do cut:
AflIII AleI AloI AlwNI ApoI BanI BanII BbsI
BbvCI BfrBI BglI BlpI Bme1580I BmrI BpmI Bpu10I
BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI
EaeI EcoICRI Eco57MI EcoRI FalI HaeII Hin4I MslI
MspA1I NcoI NsiI NspI PasI PciI PpiI PstI
PvuII SacI SfcI StuI StyI TaqII TatI XbaI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BcgI BciVI BclI
BglII BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiWI BsmBI BspEI
BsrBI BssHII BstEII BstXI BstZ17I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoNI
EcoO109I EcoRV FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PflMI PfoI
PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XhoI
XmaI XmnI ZraI