Restriction map for
pF1KB8151
(Linear) (Six-base) MAPSORT of: KIBB8151.seq Check: 4352 from: 1 to: 912
KIBB8151.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AclI AA'CG_TT
Cuts at: 0 337 912
Size: 337 575
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 891 912
Size: 515 376 21
Fragments arranged by size:
515 376 21
AflIII A'CryG_T
Cuts at: 0 867 912
Size: 867 45
AlwNI CAG_nnn'CTG
Cuts at: 0 636 912
Size: 636 276
ApaI G_GGCC'C
Cuts at: 0 659 710 912
Size: 659 51 202
Fragments arranged by size:
659 202 51
ApoI r'AATT_y
Cuts at: 0 304 912
Size: 304 608
AseI AT'TA_AT
Cuts at: 0 806 912
Size: 806 106
AvaI C'yCGr_G
Cuts at: 0 117 130 238 912
Size: 117 13 108 674
Fragments arranged by size:
674 117 108 13
BanI G'GyrC_C
Cuts at: 0 210 912
Size: 210 702
BanII G_rGCy'C
Cuts at: 0 250 417 659 710 912
Size: 250 167 242 51 202
Fragments arranged by size:
250 242 202 167 51
BglII A'GATC_T
Cuts at: 0 447 912
Size: 447 465
BlpI GC'TnA_GC
Cuts at: 0 145 912
Size: 145 767
Bme1580I G_kGCm'C
Cuts at: 0 659 710 912
Size: 659 51 202
Fragments arranged by size:
659 202 51
BmrI ACTGGGnnnn_n'
Cuts at: 0 51 646 912
Size: 51 595 266
Fragments arranged by size:
595 266 51
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 400 912
Size: 400 512
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 682 912
Size: 237 445 230
Fragments arranged by size:
445 237 230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 912
Size: 495 417
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 388 418 912
Size: 388 30 494
Fragments arranged by size:
494 388 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 409 912
Size: 37 372 503
Fragments arranged by size:
503 372 37
BseYI C'CCAG_C
Cuts at: 0 227 670 912
Size: 227 443 242
Fragments arranged by size:
443 242 227
BsiHKAI G_wGCw'C
Cuts at: 0 124 146 912
Size: 124 22 766
Fragments arranged by size:
766 124 22
BsmI GAATG_Cn'
Cuts at: 0 516 912
Size: 516 396
BsmBI CGTCTCn'nnnn_
Cuts at: 0 156 174 912
Size: 156 18 738
Fragments arranged by size:
738 156 18
Bsp1286I G_dGCh'C
Cuts at: 0 124 146 250 417 659 710 912
Size: 124 22 104 167 242 51 202
Fragments arranged by size:
242 202 167 124 104 51 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 582 912
Size: 582 330
BsrBI CCG'CTC
Cuts at: 0 27 687 912
Size: 27 660 225
Fragments arranged by size:
660 225 27
BsrDI GCAATG_nn'
Cuts at: 0 286 912
Size: 286 626
BsrFI r'CCGG_y
Cuts at: 0 207 912
Size: 207 705
BstEII G'GTnAC_C
Cuts at: 0 607 912
Size: 607 305
BstYI r'GATC_y
Cuts at: 0 358 447 912
Size: 358 89 465
Fragments arranged by size:
465 358 89
BtsI GCAGTG_nn'
Cuts at: 0 508 912
Size: 508 404
DraIII CAC_nnn'GTG
Cuts at: 0 99 640 912
Size: 99 541 272
Fragments arranged by size:
541 272 99
EarI CTCTTCn'nnn_
Cuts at: 0 491 912
Size: 491 421
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 224 415 570 912
Size: 224 191 155 342
Fragments arranged by size:
342 224 191 155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 515 682 891 912
Size: 237 278 167 209 21
Fragments arranged by size:
278 237 209 167 21
EcoO109I rG'GnC_Cy
Cuts at: 0 656 912
Size: 656 256
EcoRI G'AATT_C
Cuts at: 0 304 912
Size: 304 608
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 780 912
Size: 780 132
HaeII r_GCGC'y
Cuts at: 0 76 912
Size: 76 836
HincII GTy'rAC
Cuts at: 0 678 912
Size: 678 234
MspA1I CmG'CkG
Cuts at: 0 90 194 912
Size: 90 104 718
Fragments arranged by size:
718 104 90
NspI r_CATG'y
Cuts at: 0 871 912
Size: 871 41
PciI A'CATG_T
Cuts at: 0 867 912
Size: 867 45
PflMI CCAn_nnn'nTGG
Cuts at: 0 640 912
Size: 640 272
PfoI T'CCnGG_A
Cuts at: 0 482 912
Size: 482 430
PspOMI G'GGCC_C
Cuts at: 0 655 706 912
Size: 655 51 206
Fragments arranged by size:
655 206 51
PspXI vC'TCGA_Gb
Cuts at: 0 117 912
Size: 117 795
PstI C_TGCA'G
Cuts at: 0 83 912
Size: 83 829
SapI GCTCTTCn'nnn_
Cuts at: 0 491 912
Size: 491 421
SfcI C'TryA_G
Cuts at: 0 79 912
Size: 79 833
SgrAI Cr'CCGG_yG
Cuts at: 0 207 912
Size: 207 705
SmaI CCC'GGG
Cuts at: 0 132 240 912
Size: 132 108 672
Fragments arranged by size:
672 132 108
SmlI C'TyrA_G
Cuts at: 0 117 474 912
Size: 117 357 438
Fragments arranged by size:
438 357 117
StyI C'CwwG_G
Cuts at: 0 764 912
Size: 764 148
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 628 641 912
Size: 628 13 271
Fragments arranged by size:
628 271 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 817 849 912
Size: 817 32 63
Fragments arranged by size:
817 63 32
XhoI C'TCGA_G
Cuts at: 0 117 912
Size: 117 795
XmaI C'CCGG_G
Cuts at: 0 130 238 912
Size: 130 108 674
Fragments arranged by size:
674 130 108
XmnI GAAnn'nnTTC
Cuts at: 0 449 912
Size: 449 463
Enzymes that do cut:
AclI AcuI AflIII AlwNI ApaI ApoI AseI AvaI
BanI BanII BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BstEII BstYI BtsI DraIII
EarI EciI Eco57MI EcoO109I EcoRI FalI HaeII HincII
MspA1I NspI PciI PflMI PfoI PspOMI PspXI PstI
SapI SfcI SgrAI SmaI SmlI StyI TaqII TstI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BmgBI BmtI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BsgI BsiEI BsiWI BspEI BspHI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DrdI EaeI EagI EcoICRI EcoNI
EcoRV FseI FspI FspAI Hin4I HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI
ZraI