(Linear) (Six-base) MAPSORT of: KIBB8141.seq  Check: 383  from: 1  to: 408



KIBB8141.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AccI GT'mk_AC

Cuts at:      0     172     408 
   Size:        172     236
 
AfeI AGC'GCT

Cuts at:      0      87     408 
   Size:         87     321
 
AgeI A'CCGG_T

Cuts at:      0      34     408 
   Size:         34     374
 
AvaI C'yCGr_G

Cuts at:      0     130     408 
   Size:        130     278
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       4      38     162     196     408 
   Size:          4      34     124      34     212

  Fragments arranged by size: 

                 212     124      34      34       4
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     383     408 
   Size:        383      25
 
BmtI G_CTAG'C

Cuts at:      0     290     408 
   Size:        290     118
 
BsaWI w'CCGG_w

Cuts at:      0      34     188     408 
   Size:         34     154     220

  Fragments arranged by size: 

                 220     154      34
 
BsiEI CG_ry'CG

Cuts at:      0     176     408 
   Size:        176     232
 
BspEI T'CCGG_A

Cuts at:      0     188     408 
   Size:        188     220
 
BsrFI r'CCGG_y

Cuts at:      0      34      97     408 
   Size:         34      63     311

  Fragments arranged by size: 

                 311      63      34
 
BstBI TT'CG_AA

Cuts at:      0     314     408 
   Size:        314      94
 
Bsu36I CC'TnA_GG

Cuts at:      0     247     408 
   Size:        247     161
 
BtgI C'CryG_G

Cuts at:      0     137     408 
   Size:        137     271
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     103     408 
   Size:        103     305
 
EaeI y'GGCC_r

Cuts at:      0      62     408 
   Size:         62     346
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     204     408 
   Size:        204     204
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     216     408 
   Size:        216     192
 
HaeII r_GCGC'y

Cuts at:      0      89     145     408 
   Size:         89      56     263

  Fragments arranged by size: 

                 263      89      56
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     341     373     408 
   Size:        341      32      35

  Fragments arranged by size: 

                 341      35      32
 
HincII GTy'rAC

Cuts at:      0     173     408 
   Size:        173     235
 
MscI TGG'CCA

Cuts at:      0      64     408 
   Size:         64     344
 
MslI CAynn'nnrTG

Cuts at:      0     358     408 
   Size:        358      50
 
MspA1I CmG'CkG

Cuts at:      0      60     408 
   Size:         60     348
 
NheI G'CTAG_C

Cuts at:      0     286     408 
   Size:        286     122
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     210     408 
   Size:        210     198
 
PstI C_TGCA'G

Cuts at:      0     281     408 
   Size:        281     127
 
PvuII CAG'CTG

Cuts at:      0      60     408 
   Size:         60     348
 
SalI G'TCGA_C

Cuts at:      0     171     408 
   Size:        171     237
 
SexAI A'CCwGG_T

Cuts at:      0     299     408 
   Size:        299     109
 
SfcI C'TryA_G

Cuts at:      0     123     277     408 
   Size:        123     154     131

  Fragments arranged by size: 

                 154     131     123
 
SgrAI Cr'CCGG_yG

Cuts at:      0      34     408 
   Size:         34     374
 
SmaI CCC'GGG

Cuts at:      0     132     408 
   Size:        132     276
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     190     408 
   Size:        190     218
 
XmaI C'CCGG_G

Cuts at:      0     130     408 
   Size:        130     278
 

 Enzymes that do cut:

     AccI     AfeI     AgeI     AvaI     BcgI    BciVI     BmtI    BsaWI
    BsiEI    BspEI    BsrFI    BstBI   Bsu36I     BtgI    BtgZI     EaeI
    EcoNI     FalI    HaeII    Hin4I   HincII     MscI     MslI   MspA1I
     NheI    PflMI     PstI    PvuII     SalI    SexAI     SfcI    SgrAI
     SmaI    TaqII     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI