Restriction map for
pF1KB8139
(Linear) (Six-base) MAPSORT of: KIBB8139.seq Check: 9485 from: 1 to: 978
KIBB8139.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 286 978
Size: 286 692
AflIII A'CryG_T
Cuts at: 0 449 978
Size: 449 529
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 359 391 978
Size: 359 32 587
Fragments arranged by size:
587 359 32
AlwNI CAG_nnn'CTG
Cuts at: 0 123 978
Size: 123 855
ApaI G_GGCC'C
Cuts at: 0 116 978
Size: 116 862
AvaI C'yCGr_G
Cuts at: 0 61 978
Size: 61 917
BanI G'GyrC_C
Cuts at: 0 812 978
Size: 812 166
BanII G_rGCy'C
Cuts at: 0 116 978
Size: 116 862
BbsI GAAGACnn'nnnn_
Cuts at: 0 414 978
Size: 414 564
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 42 76 774 808 978
Size: 42 34 698 34 170
Fragments arranged by size:
698 170 42 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 143 779 978
Size: 143 636 199
Fragments arranged by size:
636 199 143
BglI GCCn_nnn'nGGC
Cuts at: 0 120 161 560 682 978
Size: 120 41 399 122 296
Fragments arranged by size:
399 296 122 120 41
Bme1580I G_kGCm'C
Cuts at: 0 116 754 978
Size: 116 638 224
Fragments arranged by size:
638 224 116
BmgBI CAC'GTC
Cuts at: 0 450 978
Size: 450 528
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 145 834 978
Size: 87 58 689 144
Fragments arranged by size:
689 144 87 58
BsaI GGTCTCn'nnnn_
Cuts at: 0 214 978
Size: 214 764
BsaAI yAC'GTr
Cuts at: 0 39 268 978
Size: 39 229 710
Fragments arranged by size:
710 229 39
BsaWI w'CCGG_w
Cuts at: 0 71 92 978
Size: 71 21 886
Fragments arranged by size:
886 71 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 359 389 978
Size: 359 30 589
Fragments arranged by size:
589 359 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 316 895 978
Size: 316 579 83
Fragments arranged by size:
579 316 83
BseYI C'CCAG_C
Cuts at: 0 116 610 937 978
Size: 116 494 327 41
Fragments arranged by size:
494 327 116 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 759 978
Size: 412 347 219
Fragments arranged by size:
412 347 219
BsmBI CGTCTCn'nnnn_
Cuts at: 0 534 978
Size: 534 444
Bsp1286I G_dGCh'C
Cuts at: 0 116 754 978
Size: 116 638 224
Fragments arranged by size:
638 224 116
BspEI T'CCGG_A
Cuts at: 0 92 978
Size: 92 886
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 286 978
Size: 286 692
BssSI C'ACGA_G
Cuts at: 0 481 531 978
Size: 481 50 447
Fragments arranged by size:
481 447 50
BstXI CCAn_nnnn'nTGG
Cuts at: 0 823 978
Size: 823 155
BstYI r'GATC_y
Cuts at: 0 65 978
Size: 65 913
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 672 822 978
Size: 672 150 156
Fragments arranged by size:
672 156 150
BtsI GCAGTG_nn'
Cuts at: 0 400 547 807 960 978
Size: 400 147 260 153 18
Fragments arranged by size:
400 260 153 147 18
EaeI y'GGCC_r
Cuts at: 0 239 561 594 683 978
Size: 239 322 33 89 295
Fragments arranged by size:
322 295 239 89 33
EarI CTCTTCn'nnn_
Cuts at: 0 43 978
Size: 43 935
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 145 834 978
Size: 87 58 689 144
Fragments arranged by size:
689 144 87 58
EcoO109I rG'GnC_Cy
Cuts at: 0 112 113 338 708 978
Size: 112 1 225 370 270
Fragments arranged by size:
370 270 225 112 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 978
Size: 130 32 816
Fragments arranged by size:
816 130 32
MscI TGG'CCA
Cuts at: 0 241 685 978
Size: 241 444 293
Fragments arranged by size:
444 293 241
MslI CAynn'nnrTG
Cuts at: 0 787 978
Size: 787 191
MspA1I CmG'CkG
Cuts at: 0 120 978
Size: 120 858
PasI CC'CwG_GG
Cuts at: 0 385 978
Size: 385 593
PflMI CCAn_nnn'nTGG
Cuts at: 0 123 978
Size: 123 855
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 359 391 978
Size: 359 32 587
Fragments arranged by size:
587 359 32
PpuMI rG'GwC_Cy
Cuts at: 0 338 708 978
Size: 338 370 270
Fragments arranged by size:
370 338 270
PshAI GACnn'nnGTC
Cuts at: 0 356 978
Size: 356 622
PspOMI G'GGCC_C
Cuts at: 0 112 978
Size: 112 866
PstI C_TGCA'G
Cuts at: 0 473 978
Size: 473 505
SanDI GG'GwC_CC
Cuts at: 0 338 978
Size: 338 640
SapI GCTCTTCn'nnn_
Cuts at: 0 43 978
Size: 43 935
SfcI C'TryA_G
Cuts at: 0 469 978
Size: 469 509
StyI C'CwwG_G
Cuts at: 0 170 215 978
Size: 170 45 763
Fragments arranged by size:
763 170 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 493 795 978
Size: 493 302 183
Fragments arranged by size:
493 302 183
Tth111I GACn'n_nGTC
Cuts at: 0 22 978
Size: 22 956
Enzymes that do cut:
AarI AflIII AloI AlwNI ApaI AvaI BanI BanII
BbsI BcgI BciVI BglI Bme1580I BmgBI BpmI BsaI
BsaAI BsaWI BsaXI BseRI BseYI BsgI BsmBI Bsp1286I
BspEI BspMI BssSI BstXI BstYI BtgZI BtsI EaeI
EarI Eco57MI EcoO109I Hin4I MscI MslI MspA1I PasI
PflMI PpiI PpuMI PshAI PspOMI PstI SanDI SapI
SfcI StyI TaqII Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BclI BfrBI BglII
BlpI BmrI BmtI BplI Bpu10I BpuEI BsaBI BsaHI
BsiEI BsiHKAI BsiWI BsmI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PmlI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI XbaI XcmI XhoI XmaI XmnI ZraI