(Linear) (Six-base) MAPSORT of: KIBB8137.seq  Check: 8951  from: 1  to: 1035



KIBB8137.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

Acc65I G'GTAC_C

Cuts at:      0     801    1035 
   Size:        801     234
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     628    1035 
   Size:        628     407
 
AfeI AGC'GCT

Cuts at:      0     729    1035 
   Size:        729     306
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     917    1035 
   Size:        917     118
 
AlwNI CAG_nnn'CTG

Cuts at:      0     561    1035 
   Size:        561     474
 
ApoI r'AATT_y

Cuts at:      0     169     699    1035 
   Size:        169     530     336

  Fragments arranged by size: 

                 530     336     169
 
AvaI C'yCGr_G

Cuts at:      0     507     596    1035 
   Size:        507      89     439

  Fragments arranged by size: 

                 507     439      89
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     351     384    1035 
   Size:        351      33     651

  Fragments arranged by size: 

                 651     351      33
 
BanI G'GyrC_C

Cuts at:      0     801    1035 
   Size:        801     234
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     616     772    1035 
   Size:        616     156     263

  Fragments arranged by size: 

                 616     263     156
 
BfrBI ATG'CAT

Cuts at:      0     528    1035 
   Size:        528     507
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     977    1035 
   Size:        977      58
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     991    1035 
   Size:        991      44
 
BsaHI Gr'CG_yC

Cuts at:      0     155     185    1035 
   Size:        155      30     850

  Fragments arranged by size: 

                 850     155      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     940    1035 
   Size:        940      95
 
BseYI C'CCAG_C

Cuts at:      0     380     730     951    1035 
   Size:        380     350     221      84

  Fragments arranged by size: 

                 380     350     221      84
 
BspHI T'CATG_A

Cuts at:      0     455    1035 
   Size:        455     580
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     622    1035 
   Size:        622     413
 
BsrGI T'GTAC_A

Cuts at:      0     412    1035 
   Size:        412     623
 
BstEII G'GTnAC_C

Cuts at:      0     288     375    1035 
   Size:        288      87     660

  Fragments arranged by size: 

                 660     288      87
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     351     915    1035 
   Size:        351     564     120

  Fragments arranged by size: 

                 564     351     120
 
BtgI C'CryG_G

Cuts at:      0     371     967    1035 
   Size:        371     596      68

  Fragments arranged by size: 

                 596     371      68
 
EaeI y'GGCC_r

Cuts at:      0     905    1035 
   Size:        905     130
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     638    1035 
   Size:        638     397
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     628    1035 
   Size:        628     407
 
EcoO109I rG'GnC_Cy

Cuts at:      0     245    1035 
   Size:        245     790
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     633    1035 
   Size:        633     402
 
HaeII r_GCGC'y

Cuts at:      0     731    1035 
   Size:        731     304
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     221     253     488     520     630     662     988    1020 
   Size:        221      32     235      32     110      32     326      32

Cuts at:   1020    1035 
   Size:         15

  Fragments arranged by size: 

                 326     235     221     110      32      32      32      32
                  15
 
KpnI G_GTAC'C

Cuts at:      0     805    1035 
   Size:        805     230
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     743    1035 
   Size:        743     292
 
MslI CAynn'nnrTG

Cuts at:      0     349     533    1035 
   Size:        349     184     502

  Fragments arranged by size: 

                 502     349     184
 
MspA1I CmG'CkG

Cuts at:      0     775    1035 
   Size:        775     260
 
NcoI C'CATG_G

Cuts at:      0     371    1035 
   Size:        371     664
 
NsiI A_TGCA'T

Cuts at:      0     530    1035 
   Size:        530     505
 
PpuMI rG'GwC_Cy

Cuts at:      0     245    1035 
   Size:        245     790
 
PshAI GACnn'nnGTC

Cuts at:      0     117    1035 
   Size:        117     918
 
PstI C_TGCA'G

Cuts at:      0     119    1035 
   Size:        119     916
 
PvuII CAG'CTG

Cuts at:      0     775    1035 
   Size:        775     260
 
SfcI C'TryA_G

Cuts at:      0     115    1035 
   Size:        115     920
 
SmlI C'TyrA_G

Cuts at:      0     992    1035 
   Size:        992      43
 
SspI AAT'ATT

Cuts at:      0     542    1035 
   Size:        542     493
 
StyI C'CwwG_G

Cuts at:      0     371     404    1035 
   Size:        371      33     631

  Fragments arranged by size: 

                 631     371      33
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     307     394    1035 
   Size:        307      87     641

  Fragments arranged by size: 

                 641     307      87
 
TatI w'GTAC_w

Cuts at:      0     412    1035 
   Size:        412     623
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     325     357    1035 
   Size:        325      32     678

  Fragments arranged by size: 

                 678     325      32
 

 Enzymes that do cut:

   Acc65I     AcuI     AfeI     AlfI    AlwNI     ApoI     AvaI     BaeI
     BanI     BbsI    BfrBI    BpuEI     BsaI    BsaHI    BseRI    BseYI
    BspHI    BspMI    BsrGI   BstEII    BstXI     BtgI     EaeI     EciI
  Eco57MI EcoO109I     FalI    HaeII    Hin4I     KpnI     MmeI     MslI
   MspA1I     NcoI     NsiI    PpuMI    PshAI     PstI    PvuII     SfcI
     SmlI     SspI     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BsaAI    BsaBI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     MfeI     MluI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI