Restriction map for pF1KB8134
 (Linear) (Six-base) MAPSORT of: KIBB8134.seq  Check: 9441  from: 1  to: 846



KIBB8134.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     166     846 
   Size:        166     680
 
AccI GT'mk_AC

Cuts at:      0      74     846 
   Size:         74     772
 
Acc65I G'GTAC_C

Cuts at:      0     671     846 
   Size:        671     175
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     769     846 
   Size:        769      77
 
AlwNI CAG_nnn'CTG

Cuts at:      0     726     846 
   Size:        726     120
 
ApaI G_GGCC'C

Cuts at:      0      42     846 
   Size:         42     804
 
AvaI C'yCGr_G

Cuts at:      0     617     846 
   Size:        617     229
 
BanI G'GyrC_C

Cuts at:      0     256     671     846 
   Size:        256     415     175

  Fragments arranged by size: 

                 415     256     175
 
BanII G_rGCy'C

Cuts at:      0      42     138     170     846 
   Size:         42      96      32     676

  Fragments arranged by size: 

                 676      96      42      32
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     344     846 
   Size:        344     502
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     462     846 
   Size:        462     384
 
BglI GCCn_nnn'nGGC

Cuts at:      0     109     846 
   Size:        109     737
 
Bme1580I G_kGCm'C

Cuts at:      0      42     846 
   Size:         42     804
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     160     256     727     748     846 
   Size:         46     114      96     471      21      98

  Fragments arranged by size: 

                 471     114      98      96      46      21
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     298     846 
   Size:        298     548
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     638     846 
   Size:        638     208
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     700     730     846 
   Size:        700      30     116

  Fragments arranged by size: 

                 700     116      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     217     301     551     846 
   Size:        217      84     250     295

  Fragments arranged by size: 

                 295     250     217      84
 
BseYI C'CCAG_C

Cuts at:      0      48      60     452     846 
   Size:         48      12     392     394

  Fragments arranged by size: 

                 394     392      48      12
 
Bsp1286I G_dGCh'C

Cuts at:      0      42     138     170     846 
   Size:         42      96      32     676

  Fragments arranged by size: 

                 676      96      42      32
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     166     806     846 
   Size:        166     640      40

  Fragments arranged by size: 

                 640     166      40
 
BstYI r'GATC_y

Cuts at:      0     779     846 
   Size:        779      67
 
BtgI C'CryG_G

Cuts at:      0     767     846 
   Size:        767      79
 
EaeI y'GGCC_r

Cuts at:      0     485     846 
   Size:        485     361
 
EarI CTCTTCn'nnn_

Cuts at:      0     634     846 
   Size:        634     212
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     160     256     727     748     769     846 
   Size:         46     114      96     471      21      21      77

  Fragments arranged by size: 

                 471     114      96      77      46      21      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     316     846 
   Size:        316     530
 
EcoO109I rG'GnC_Cy

Cuts at:      0      38      39     123     702     795     833     846 
   Size:         38       1      84     579      93      38      13

  Fragments arranged by size: 

                 579      93      84      38      38      13       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     172     204     519     551     846 
   Size:        172      32     315      32     295

  Fragments arranged by size: 

                 315     295     172      32      32
 
KpnI G_GTAC'C

Cuts at:      0     675     846 
   Size:        675     171
 
MscI TGG'CCA

Cuts at:      0     487     846 
   Size:        487     359
 
MspA1I CmG'CkG

Cuts at:      0     681     846 
   Size:        681     165
 
NruI TCG'CGA

Cuts at:      0     743     846 
   Size:        743     103
 
PasI CC'CwG_GG

Cuts at:      0      18     846 
   Size:         18     828
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      55     726     846 
   Size:         55     671     120

  Fragments arranged by size: 

                 671     120      55
 
PpuMI rG'GwC_Cy

Cuts at:      0     123     795     833     846 
   Size:        123     672      38      13

  Fragments arranged by size: 

                 672     123      38      13
 
PspOMI G'GGCC_C

Cuts at:      0      38     846 
   Size:         38     808
 
SmlI C'TyrA_G

Cuts at:      0     313     846 
   Size:        313     533
 
StyI C'CwwG_G

Cuts at:      0     126     846 
   Size:        126     720
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     700     732     846 
   Size:        700      32     114

  Fragments arranged by size: 

                 700     114      32
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI    AlwNI     ApaI     AvaI     BanI
    BanII     BbsI    BciVI     BglI Bme1580I     BpmI    BpuEI     BsaI
    BsaXI    BseRI    BseYI Bsp1286I    BspMI    BstYI     BtgI     EaeI
     EarI  Eco57MI    EcoNI EcoO109I    Hin4I     KpnI     MscI   MspA1I
     NruI     PasI    PflMI    PpuMI   PspOMI     SmlI     StyI     TstI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI     BclI    BfrBI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BsaAI    BsaBI
    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     MfeI     MluI     MmeI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NsiI     NspI
     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
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