Restriction map for
pF1KB8128
(Linear) (Six-base) MAPSORT of: KIBB8128.seq Check: 4034 from: 1 to: 924
KIBB8128.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AccI GT'mk_AC
Cuts at: 0 554 924
Size: 554 370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 716 924
Size: 393 323 208
Fragments arranged by size:
393 323 208
ApaI G_GGCC'C
Cuts at: 0 115 924
Size: 115 809
ApoI r'AATT_y
Cuts at: 0 349 498 615 638 924
Size: 349 149 117 23 286
Fragments arranged by size:
349 286 149 117 23
AvaI C'yCGr_G
Cuts at: 0 253 924
Size: 253 671
BanII G_rGCy'C
Cuts at: 0 115 744 924
Size: 115 629 180
Fragments arranged by size:
629 180 115
BciVI GTATCCnnnnn_n'
Cuts at: 0 242 734 924
Size: 242 492 190
Fragments arranged by size:
492 242 190
BclI T'GATC_A
Cuts at: 0 675 924
Size: 675 249
Bme1580I G_kGCm'C
Cuts at: 0 115 924
Size: 115 809
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 924
Size: 901 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 889 924
Size: 889 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 798 806 907 924
Size: 798 8 101 17
Fragments arranged by size:
798 101 17 8
BsiHKAI G_wGCw'C
Cuts at: 0 744 924
Size: 744 180
Bsp1286I G_dGCh'C
Cuts at: 0 115 744 924
Size: 115 629 180
Fragments arranged by size:
629 180 115
BsrGI T'GTAC_A
Cuts at: 0 713 804 924
Size: 713 91 120
Fragments arranged by size:
713 120 91
BstEII G'GTnAC_C
Cuts at: 0 208 924
Size: 208 716
BstXI CCAn_nnnn'nTGG
Cuts at: 0 242 924
Size: 242 682
BtgI C'CryG_G
Cuts at: 0 828 924
Size: 828 96
EaeI y'GGCC_r
Cuts at: 0 845 924
Size: 845 79
EarI CTCTTCn'nnn_
Cuts at: 0 732 924
Size: 732 192
EcoICRI GAG'CTC
Cuts at: 0 742 924
Size: 742 182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 716 924
Size: 393 323 208
Fragments arranged by size:
393 323 208
EcoO109I rG'GnC_Cy
Cuts at: 0 112 924
Size: 112 812
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 328 916 924
Size: 328 588 8
Fragments arranged by size:
588 328 8
HaeII r_GCGC'y
Cuts at: 0 323 924
Size: 323 601
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 177 209 924
Size: 177 32 715
Fragments arranged by size:
715 177 32
MfeI C'AATT_G
Cuts at: 0 295 924
Size: 295 629
MslI CAynn'nnrTG
Cuts at: 0 774 924
Size: 774 150
NcoI C'CATG_G
Cuts at: 0 828 924
Size: 828 96
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 32 64 924
Size: 32 32 860
Fragments arranged by size:
860 32 32
PsiI TTA'TAA
Cuts at: 0 29 924
Size: 29 895
PspOMI G'GGCC_C
Cuts at: 0 111 924
Size: 111 813
PstI C_TGCA'G
Cuts at: 0 168 924
Size: 168 756
SacI G_AGCT'C
Cuts at: 0 744 924
Size: 744 180
SapI GCTCTTCn'nnn_
Cuts at: 0 732 924
Size: 732 192
SfcI C'TryA_G
Cuts at: 0 164 924
Size: 164 760
SmlI C'TyrA_G
Cuts at: 0 253 880 924
Size: 253 627 44
Fragments arranged by size:
627 253 44
StyI C'CwwG_G
Cuts at: 0 828 900 924
Size: 828 72 24
Fragments arranged by size:
828 72 24
TatI w'GTAC_w
Cuts at: 0 713 804 924
Size: 713 91 120
Fragments arranged by size:
713 120 91
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 546 924
Size: 546 378
XhoI C'TCGA_G
Cuts at: 0 253 924
Size: 253 671
XmnI GAAnn'nnTTC
Cuts at: 0 501 924
Size: 501 423
Enzymes that do cut:
AccI AcuI ApaI ApoI AvaI BanII BciVI BclI
Bme1580I BpuEI BseRI BsgI BsiHKAI Bsp1286I BsrGI BstEII
BstXI BtgI EaeI EarI EcoICRI Eco57MI EcoO109I FalI
HaeII Hin4I MfeI MslI NcoI PpiI PsiI PspOMI
PstI SacI SapI SfcI SmlI StyI TatI XcmI
XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpuMI PshAI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XmaI ZraI