Restriction map for
pF1KB8121
(Linear) (Six-base) MAPSORT of: KIBB8121.seq Check: 5312 from: 1 to: 960
KIBB8121.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 648 960
Size: 368 280 312
Fragments arranged by size:
368 312 280
AfeI AGC'GCT
Cuts at: 0 157 960
Size: 157 803
AleI CACnn'nnGTG
Cuts at: 0 521 960
Size: 521 439
ApoI r'AATT_y
Cuts at: 0 813 837 960
Size: 813 24 123
Fragments arranged by size:
813 123 24
BanI G'GyrC_C
Cuts at: 0 295 960
Size: 295 665
BanII G_rGCy'C
Cuts at: 0 29 960
Size: 29 931
BbsI GAAGACnn'nnnn_
Cuts at: 0 347 800 960
Size: 347 453 160
Fragments arranged by size:
453 347 160
BbvCI CC'TCA_GC
Cuts at: 0 37 960
Size: 37 923
BglI GCCn_nnn'nGGC
Cuts at: 0 866 960
Size: 866 94
Bme1580I G_kGCm'C
Cuts at: 0 300 960
Size: 300 660
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 255 960
Size: 144 111 705
Fragments arranged by size:
705 144 111
Bpu10I CC'TnA_GC
Cuts at: 0 37 960
Size: 37 923
BsaBI GATnn'nnATC
Cuts at: 0 403 960
Size: 403 557
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 454 484 960
Size: 454 30 476
Fragments arranged by size:
476 454 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 105 211 960
Size: 105 106 749
Fragments arranged by size:
749 106 105
BsiEI CG_ry'CG
Cuts at: 0 651 960
Size: 651 309
BsmI GAATG_Cn'
Cuts at: 0 222 960
Size: 222 738
Bsp1286I G_dGCh'C
Cuts at: 0 29 300 960
Size: 29 271 660
Fragments arranged by size:
660 271 29
BspHI T'CATG_A
Cuts at: 0 407 960
Size: 407 553
BsrDI GCAATG_nn'
Cuts at: 0 112 960
Size: 112 848
BstYI r'GATC_y
Cuts at: 0 141 960
Size: 141 819
Bsu36I CC'TnA_GG
Cuts at: 0 88 960
Size: 88 872
BtgI C'CryG_G
Cuts at: 0 473 693 960
Size: 473 220 267
Fragments arranged by size:
473 267 220
BtsI GCAGTG_nn'
Cuts at: 0 429 723 951 960
Size: 429 294 228 9
Fragments arranged by size:
429 294 228 9
DraIII CAC_nnn'GTG
Cuts at: 0 234 960
Size: 234 726
EaeI y'GGCC_r
Cuts at: 0 513 960
Size: 513 447
EarI CTCTTCn'nnn_
Cuts at: 0 52 237 960
Size: 52 185 723
Fragments arranged by size:
723 185 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 255 368 648 960
Size: 144 111 113 280 312
Fragments arranged by size:
312 280 144 113 111
HaeII r_GCGC'y
Cuts at: 0 159 960
Size: 159 801
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 454 486 960
Size: 454 32 474
Fragments arranged by size:
474 454 32
MscI TGG'CCA
Cuts at: 0 515 960
Size: 515 445
MslI CAynn'nnrTG
Cuts at: 0 521 960
Size: 521 439
MspA1I CmG'CkG
Cuts at: 0 36 960
Size: 36 924
NdeI CA'TA_TG
Cuts at: 0 183 960
Size: 183 777
PvuII CAG'CTG
Cuts at: 0 36 960
Size: 36 924
SapI GCTCTTCn'nnn_
Cuts at: 0 52 960
Size: 52 908
SexAI A'CCwGG_T
Cuts at: 0 291 960
Size: 291 669
StyI C'CwwG_G
Cuts at: 0 903 960
Size: 903 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 431 637 960
Size: 431 206 323
Fragments arranged by size:
431 323 206
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 233 265 960
Size: 233 32 695
Fragments arranged by size:
695 233 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 508 910 960
Size: 508 402 50
Fragments arranged by size:
508 402 50
XmnI GAAnn'nnTTC
Cuts at: 0 46 591 960
Size: 46 545 369
Fragments arranged by size:
545 369 46
Enzymes that do cut:
AcuI AfeI AleI ApoI BanI BanII BbsI BbvCI
BglI Bme1580I BpmI Bpu10I BsaBI BsaXI BseRI BsiEI
BsmI Bsp1286I BspHI BsrDI BstYI Bsu36I BtgI BtsI
DraIII EaeI EarI Eco57MI HaeII Hin4I MscI MslI
MspA1I NdeI PvuII SapI SexAI StyI TaqII TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
BpuEI BsaI BsaAI BsaHI BsaWI BseYI BsgI BsiHKAI
BsiWI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI
CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI
XmaI ZraI