Restriction map for pF1KB8121
 (Linear) (Six-base) MAPSORT of: KIBB8121.seq  Check: 5312  from: 1  to: 960



KIBB8121.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     368     648     960 
   Size:        368     280     312

  Fragments arranged by size: 

                 368     312     280
 
AfeI AGC'GCT

Cuts at:      0     157     960 
   Size:        157     803
 
AleI CACnn'nnGTG

Cuts at:      0     521     960 
   Size:        521     439
 
ApoI r'AATT_y

Cuts at:      0     813     837     960 
   Size:        813      24     123

  Fragments arranged by size: 

                 813     123      24
 
BanI G'GyrC_C

Cuts at:      0     295     960 
   Size:        295     665
 
BanII G_rGCy'C

Cuts at:      0      29     960 
   Size:         29     931
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     347     800     960 
   Size:        347     453     160

  Fragments arranged by size: 

                 453     347     160
 
BbvCI CC'TCA_GC

Cuts at:      0      37     960 
   Size:         37     923
 
BglI GCCn_nnn'nGGC

Cuts at:      0     866     960 
   Size:        866      94
 
Bme1580I G_kGCm'C

Cuts at:      0     300     960 
   Size:        300     660
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     255     960 
   Size:        144     111     705

  Fragments arranged by size: 

                 705     144     111
 
Bpu10I CC'TnA_GC

Cuts at:      0      37     960 
   Size:         37     923
 
BsaBI GATnn'nnATC

Cuts at:      0     403     960 
   Size:        403     557
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     454     484     960 
   Size:        454      30     476

  Fragments arranged by size: 

                 476     454      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     105     211     960 
   Size:        105     106     749

  Fragments arranged by size: 

                 749     106     105
 
BsiEI CG_ry'CG

Cuts at:      0     651     960 
   Size:        651     309
 
BsmI GAATG_Cn'

Cuts at:      0     222     960 
   Size:        222     738
 
Bsp1286I G_dGCh'C

Cuts at:      0      29     300     960 
   Size:         29     271     660

  Fragments arranged by size: 

                 660     271      29
 
BspHI T'CATG_A

Cuts at:      0     407     960 
   Size:        407     553
 
BsrDI GCAATG_nn'

Cuts at:      0     112     960 
   Size:        112     848
 
BstYI r'GATC_y

Cuts at:      0     141     960 
   Size:        141     819
 
Bsu36I CC'TnA_GG

Cuts at:      0      88     960 
   Size:         88     872
 
BtgI C'CryG_G

Cuts at:      0     473     693     960 
   Size:        473     220     267

  Fragments arranged by size: 

                 473     267     220
 
BtsI GCAGTG_nn'

Cuts at:      0     429     723     951     960 
   Size:        429     294     228       9

  Fragments arranged by size: 

                 429     294     228       9
 
DraIII CAC_nnn'GTG

Cuts at:      0     234     960 
   Size:        234     726
 
EaeI y'GGCC_r

Cuts at:      0     513     960 
   Size:        513     447
 
EarI CTCTTCn'nnn_

Cuts at:      0      52     237     960 
   Size:         52     185     723

  Fragments arranged by size: 

                 723     185      52
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     255     368     648     960 
   Size:        144     111     113     280     312

  Fragments arranged by size: 

                 312     280     144     113     111
 
HaeII r_GCGC'y

Cuts at:      0     159     960 
   Size:        159     801
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     454     486     960 
   Size:        454      32     474

  Fragments arranged by size: 

                 474     454      32
 
MscI TGG'CCA

Cuts at:      0     515     960 
   Size:        515     445
 
MslI CAynn'nnrTG

Cuts at:      0     521     960 
   Size:        521     439
 
MspA1I CmG'CkG

Cuts at:      0      36     960 
   Size:         36     924
 
NdeI CA'TA_TG

Cuts at:      0     183     960 
   Size:        183     777
 
PvuII CAG'CTG

Cuts at:      0      36     960 
   Size:         36     924
 
SapI GCTCTTCn'nnn_

Cuts at:      0      52     960 
   Size:         52     908
 
SexAI A'CCwGG_T

Cuts at:      0     291     960 
   Size:        291     669
 
StyI C'CwwG_G

Cuts at:      0     903     960 
   Size:        903      57
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     431     637     960 
   Size:        431     206     323

  Fragments arranged by size: 

                 431     323     206
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     233     265     960 
   Size:        233      32     695

  Fragments arranged by size: 

                 695     233      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     508     910     960 
   Size:        508     402      50

  Fragments arranged by size: 

                 508     402      50
 
XmnI GAAnn'nnTTC

Cuts at:      0      46     591     960 
   Size:         46     545     369

  Fragments arranged by size: 

                 545     369      46
 

 Enzymes that do cut:

     AcuI     AfeI     AleI     ApoI     BanI    BanII     BbsI    BbvCI
     BglI Bme1580I     BpmI   Bpu10I    BsaBI    BsaXI    BseRI    BsiEI
     BsmI Bsp1286I    BspHI    BsrDI    BstYI   Bsu36I     BtgI     BtsI
   DraIII     EaeI     EarI  Eco57MI    HaeII    Hin4I     MscI     MslI
   MspA1I     NdeI    PvuII     SapI    SexAI     StyI    TaqII     TstI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BseYI     BsgI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     ClaI
    CspCI     DraI     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XhoI
     XmaI     ZraI
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