(Linear) (Six-base) MAPSORT of: KIBB8109.seq  Check: 7444  from: 1  to: 459



KIBB8109.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AccI GT'mk_AC

Cuts at:      0     227     459 
   Size:        227     232
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     422     459 
   Size:        422      37
 
AvaI C'yCGr_G

Cuts at:      0     140     459 
   Size:        140     319
 
BanI G'GyrC_C

Cuts at:      0     426     459 
   Size:        426      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      76      87     459 
   Size:         76      11     372

  Fragments arranged by size: 

                 372      76      11
 
BglII A'GATC_T

Cuts at:      0     170     459 
   Size:        170     289
 
Bme1580I G_kGCm'C

Cuts at:      0     253     429     459 
   Size:        253     176      30

  Fragments arranged by size: 

                 253     176      30
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     444     459 
   Size:        444      15
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     121     151     459 
   Size:        121      30     308

  Fragments arranged by size: 

                 308     121      30
 
BsiEI CG_ry'CG

Cuts at:      0      59      63     114     459 
   Size:         59       4      51     345

  Fragments arranged by size: 

                 345      59      51       4
 
Bsp1286I G_dGCh'C

Cuts at:      0     253     429     459 
   Size:        253     176      30

  Fragments arranged by size: 

                 253     176      30
 
BstYI r'GATC_y

Cuts at:      0     170     459 
   Size:        170     289
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     366     459 
   Size:        366      93
 
EaeI y'GGCC_r

Cuts at:      0     111     459 
   Size:        111     348
 
EagI C'GGCC_G

Cuts at:      0     111     459 
   Size:        111     348
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     422     459 
   Size:        422      37
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     437     459 
   Size:        437      22
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     121     153     459 
   Size:        121      32     306

  Fragments arranged by size: 

                 306     121      32
 
HindIII A'AGCT_T

Cuts at:      0     210     459 
   Size:        210     249
 
MslI CAynn'nnrTG

Cuts at:      0     181     459 
   Size:        181     278
 
NspI r_CATG'y

Cuts at:      0      77     459 
   Size:         77     382
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     126     459 
   Size:        126     333
 
PstI C_TGCA'G

Cuts at:      0     368     459 
   Size:        368      91
 
PvuI CG_AT'CG

Cuts at:      0      59     459 
   Size:         59     400
 
SfcI C'TryA_G

Cuts at:      0     364     459 
   Size:        364      95
 
SmaI CCC'GGG

Cuts at:      0     142     459 
   Size:        142     317
 
XmaI C'CCGG_G

Cuts at:      0     140     459 
   Size:        140     319
 

 Enzymes that do cut:

     AccI     AcuI     AvaI     BanI     BbsI    BglII Bme1580I     BsaI
    BsaXI    BsiEI Bsp1286I    BstYI     DrdI     EaeI     EagI  Eco57MI
    EcoNI    Hin4I  HindIII     MslI     NspI    PflMI     PstI     PvuI
     SfcI     SmaI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EarI
     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI