Restriction map for
pF1KB8031
(Linear) (Six-base) MAPSORT of: KIBB8031.seq Check: 1850 from: 1 to: 1758
KIBB8031.orf
With 182 enzymes: SgfI *
May 21, 2013 16:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 956 1590 1758
Size: 956 634 168
Fragments arranged by size:
956 634 168
AflIII A'CryG_T
Cuts at: 0 950 1758
Size: 950 808
AlwNI CAG_nnn'CTG
Cuts at: 0 384 729 777 1758
Size: 384 345 48 981
Fragments arranged by size:
981 384 345 48
ApaI G_GGCC'C
Cuts at: 0 405 1758
Size: 405 1353
ApaLI G'TGCA_C
Cuts at: 0 374 1758
Size: 374 1384
AvaI C'yCGr_G
Cuts at: 0 5 326 354 421 793 1046 1307 1758
Size: 5 321 28 67 372 253 261 451
Fragments arranged by size:
451 372 321 261 253 67 28 5
BanI G'GyrC_C
Cuts at: 0 336 417 816 865 1318 1413 1758
Size: 336 81 399 49 453 95 345
Fragments arranged by size:
453 399 345 336 95 81 49
BanII G_rGCy'C
Cuts at: 0 196 405 431 1283 1362 1481 1601 1758
Size: 196 209 26 852 79 119 120 157
Fragments arranged by size:
852 209 196 157 120 119 79 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 693 1758
Size: 693 1065
BbvCI CC'TCA_GC
Cuts at: 0 538 1758
Size: 538 1220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 219 253 1758
Size: 219 34 1505
Fragments arranged by size:
1505 219 34
BclI T'GATC_A
Cuts at: 0 1427 1758
Size: 1427 331
BglI GCCn_nnn'nGGC
Cuts at: 0 461 1758
Size: 461 1297
BlpI GC'TnA_GC
Cuts at: 0 1117 1758
Size: 1117 641
Bme1580I G_kGCm'C
Cuts at: 0 378 397 405 422 821 1418 1758
Size: 378 19 8 17 399 597 340
Fragments arranged by size:
597 399 378 340 19 17 8
BmgBI CAC'GTC
Cuts at: 0 1163 1758
Size: 1163 595
BmrI ACTGGGnnnn_n'
Cuts at: 0 399 400 1375 1758
Size: 399 1 975 383
Fragments arranged by size:
975 399 383 1
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 196 1758
Size: 196 1562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 691 832 1018 1758
Size: 166 525 141 186 740
Fragments arranged by size:
740 525 186 166 141
Bpu10I CC'TnA_GC
Cuts at: 0 538 1758
Size: 538 1220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 1758
Size: 961 797
BsaI GGTCTCn'nnnn_
Cuts at: 0 667 1057 1758
Size: 667 390 701
Fragments arranged by size:
701 667 390
BsaHI Gr'CG_yC
Cuts at: 0 583 1758
Size: 583 1175
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1226 1256 1334 1364 1758
Size: 1226 30 78 30 394
Fragments arranged by size:
1226 394 78 30 30
BseYI C'CCAG_C
Cuts at: 0 39 717 1005 1056 1073 1179 1758
Size: 39 678 288 51 17 106 579
Fragments arranged by size:
678 579 288 106 51 39 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 535 638 1639 1758
Size: 213 322 103 1001 119
Fragments arranged by size:
1001 322 213 119 103
BsiHKAI G_wGCw'C
Cuts at: 0 378 431 1283 1362 1481 1758
Size: 378 53 852 79 119 277
Fragments arranged by size:
852 378 277 119 79 53
BsmI GAATG_Cn'
Cuts at: 0 1044 1758
Size: 1044 714
BsmBI CGTCTCn'nnnn_
Cuts at: 0 325 964 1725 1758
Size: 325 639 761 33
Fragments arranged by size:
761 639 325 33
Bsp1286I G_dGCh'C
Cuts at: 0 196 378 397 405 422 431 821 1283
Size: 196 182 19 8 17 9 390 462
Cuts at: 1283 1362 1418 1481 1601 1758
Size: 79 56 63 120 157
Fragments arranged by size:
462 390 196 182 157 120 79 63
56 19 17 9 8
BspHI T'CATG_A
Cuts at: 0 1508 1758
Size: 1508 250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1061 1758
Size: 1061 697
BsrBI CCG'CTC
Cuts at: 0 1543 1758
Size: 1543 215
BsrDI GCAATG_nn'
Cuts at: 0 740 1547 1758
Size: 740 807 211
Fragments arranged by size:
807 740 211
BsrFI r'CCGG_y
Cuts at: 0 339 1758
Size: 339 1419
BstAPI GCAn_nnn'nTGC
Cuts at: 0 865 1758
Size: 865 893
BstXI CCAn_nnnn'nTGG
Cuts at: 0 647 669 1639 1758
Size: 647 22 970 119
Fragments arranged by size:
970 647 119 22
Bsu36I CC'TnA_GG
Cuts at: 0 625 632 1729 1758
Size: 625 7 1097 29
Fragments arranged by size:
1097 625 29 7
BtgI C'CryG_G
Cuts at: 0 20 200 432 459 646 677 1149 1199
Size: 20 180 232 27 187 31 472 50
Cuts at: 1199 1252 1409 1638 1758
Size: 53 157 229 120
Fragments arranged by size:
472 232 229 187 180 157 120 53
50 31 27 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 198 483 1252 1314 1758
Size: 198 285 769 62 444
Fragments arranged by size:
769 444 285 198 62
ClaI AT'CG_AT
Cuts at: 0 1094 1758
Size: 1094 664
DrdI GACnn_nn'nnGTC
Cuts at: 0 151 1161 1758
Size: 151 1010 597
Fragments arranged by size:
1010 597 151
EaeI y'GGCC_r
Cuts at: 0 341 462 659 680 779 926 1098 1133
Size: 341 121 197 21 99 147 172 35
Cuts at: 1133 1544 1641 1758
Size: 411 97 117
Fragments arranged by size:
411 341 197 172 147 121 117 99
97 35 21
EarI CTCTTCn'nnn_
Cuts at: 0 607 1729 1758
Size: 607 1122 29
Fragments arranged by size:
1122 607 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 281 1072 1758
Size: 281 791 686
Fragments arranged by size:
791 686 281
EcoICRI GAG'CTC
Cuts at: 0 429 1281 1360 1479 1758
Size: 429 852 79 119 279
Fragments arranged by size:
852 429 279 119 79
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 691 832 956 1018 1590 1758
Size: 166 525 141 124 62 572 168
Fragments arranged by size:
572 525 168 166 141 124 62
EcoO109I rG'GnC_Cy
Cuts at: 0 99 401 636 774 1011 1050 1661 1703
Size: 99 302 235 138 237 39 611 42
Cuts at: 1703 1758
Size: 55
Fragments arranged by size:
611 302 237 235 138 99 55 42
39
HaeII r_GCGC'y
Cuts at: 0 58 1199 1758
Size: 58 1141 559
Fragments arranged by size:
1141 559 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 161 193 260 292 1758
Size: 161 32 67 32 1466
Fragments arranged by size:
1466 161 67 32 32
HincII GTy'rAC
Cuts at: 0 243 587 1572 1758
Size: 243 344 985 186
Fragments arranged by size:
985 344 243 186
MscI TGG'CCA
Cuts at: 0 661 682 781 928 1643 1758
Size: 661 21 99 147 715 115
Fragments arranged by size:
715 661 147 115 99 21
MslI CAynn'nnrTG
Cuts at: 0 645 1758
Size: 645 1113
MspA1I CmG'CkG
Cuts at: 0 483 762 1758
Size: 483 279 996
Fragments arranged by size:
996 483 279
NaeI GCC'GGC
Cuts at: 0 341 1758
Size: 341 1417
NcoI C'CATG_G
Cuts at: 0 459 646 1409 1758
Size: 459 187 763 349
Fragments arranged by size:
763 459 349 187
NgoMIV G'CCGG_C
Cuts at: 0 339 1758
Size: 339 1419
NspI r_CATG'y
Cuts at: 0 523 1570 1758
Size: 523 1047 188
Fragments arranged by size:
1047 523 188
PasI CC'CwG_GG
Cuts at: 0 473 822 1440 1758
Size: 473 349 618 318
Fragments arranged by size:
618 473 349 318
PflMI CCAn_nnn'nTGG
Cuts at: 0 729 1758
Size: 729 1029
PfoI T'CCnGG_A
Cuts at: 0 1283 1362 1758
Size: 1283 79 396
Fragments arranged by size:
1283 396 79
PpuMI rG'GwC_Cy
Cuts at: 0 636 774 1011 1050 1758
Size: 636 138 237 39 708
Fragments arranged by size:
708 636 237 138 39
PspOMI G'GGCC_C
Cuts at: 0 401 1758
Size: 401 1357
PstI C_TGCA'G
Cuts at: 0 773 861 1758
Size: 773 88 897
Fragments arranged by size:
897 773 88
PvuII CAG'CTG
Cuts at: 0 483 762 1758
Size: 483 279 996
Fragments arranged by size:
996 483 279
SacI G_AGCT'C
Cuts at: 0 431 1283 1362 1481 1758
Size: 431 852 79 119 277
Fragments arranged by size:
852 431 277 119 79
SanDI GG'GwC_CC
Cuts at: 0 1011 1758
Size: 1011 747
SapI GCTCTTCn'nnn_
Cuts at: 0 607 1758
Size: 607 1151
SbfI CC_TGCA'GG
Cuts at: 0 861 1758
Size: 861 897
SfcI C'TryA_G
Cuts at: 0 769 857 1125 1758
Size: 769 88 268 633
Fragments arranged by size:
769 633 268 88
SmaI CCC'GGG
Cuts at: 0 7 328 423 1048 1309 1758
Size: 7 321 95 625 261 449
Fragments arranged by size:
625 449 321 261 95 7
SmlI C'TyrA_G
Cuts at: 0 976 1758
Size: 976 782
StuI AGG'CCT
Cuts at: 0 118 630 933 1141 1758
Size: 118 512 303 208 617
Fragments arranged by size:
617 512 303 208 118
StyI C'CwwG_G
Cuts at: 0 149 459 646 746 986 1409 1758
Size: 149 310 187 100 240 423 349
Fragments arranged by size:
423 349 310 240 187 149 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1342 1758
Size: 1342 416
TatI w'GTAC_w
Cuts at: 0 839 1758
Size: 839 919
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1226 1258 1334 1366 1758
Size: 1226 32 76 32 392
Fragments arranged by size:
1226 392 76 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 103 219 457 690 1093 1758
Size: 103 116 238 233 403 665
Fragments arranged by size:
665 403 238 233 116 103
XmaI C'CCGG_G
Cuts at: 0 5 326 421 1046 1307 1758
Size: 5 321 95 625 261 451
Fragments arranged by size:
625 451 321 261 95 5
Enzymes that do cut:
AcuI AflIII AlwNI ApaI ApaLI AvaI BanI BanII
BbsI BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BstAPI BstXI Bsu36I BtgI BtgZI
ClaI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
HaeII Hin4I HincII MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PstI
PvuII SacI SanDI SapI SbfI SfcI SmaI SmlI
StuI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BciVI BfrBI BglII BmtI
BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BspEI BsrGI
BssHII BssSI BstBI BstEII BstYI BstZ17I BtsI CspCI
DraI DraIII EagI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
MmeI NarI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XhoI XmnI ZraI