Restriction map for
pF1KB8018
(Linear) (Six-base) MAPSORT of: KIBB8018.seq Check: 8071 from: 1 to: 1665
KIBB8018.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1499 1665
Size: 1499 166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1021 1665
Size: 1021 644
AflII C'TTAA_G
Cuts at: 0 865 1665
Size: 865 800
AflIII A'CryG_T
Cuts at: 0 702 1665
Size: 702 963
AgeI A'CCGG_T
Cuts at: 0 1445 1665
Size: 1445 220
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1607 1639 1665
Size: 1607 32 26
Fragments arranged by size:
1607 32 26
AlwNI CAG_nnn'CTG
Cuts at: 0 120 141 601 1665
Size: 120 21 460 1064
Fragments arranged by size:
1064 460 120 21
ApaLI G'TGCA_C
Cuts at: 0 209 1665
Size: 209 1456
ApoI r'AATT_y
Cuts at: 0 726 1152 1665
Size: 726 426 513
Fragments arranged by size:
726 513 426
AvaI C'yCGr_G
Cuts at: 0 1067 1349 1665
Size: 1067 282 316
Fragments arranged by size:
1067 316 282
BanI G'GyrC_C
Cuts at: 0 385 1665
Size: 385 1280
BanII G_rGCy'C
Cuts at: 0 25 380 423 1432 1665
Size: 25 355 43 1009 233
Fragments arranged by size:
1009 355 233 43 25
BbeI G_GCGC'C
Cuts at: 0 389 1665
Size: 389 1276
BbvCI CC'TCA_GC
Cuts at: 0 1590 1665
Size: 1590 75
BciVI GTATCCnnnnn_n'
Cuts at: 0 984 1665
Size: 984 681
BglI GCCn_nnn'nGGC
Cuts at: 0 394 403 1665
Size: 394 9 1262
Fragments arranged by size:
1262 394 9
BglII A'GATC_T
Cuts at: 0 742 1527 1665
Size: 742 785 138
Fragments arranged by size:
785 742 138
Bme1580I G_kGCm'C
Cuts at: 0 213 1665
Size: 213 1452
BmrI ACTGGGnnnn_n'
Cuts at: 0 963 1665
Size: 963 702
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1449 1451 1665
Size: 1449 2 214
Fragments arranged by size:
1449 214 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 646 1437 1665
Size: 163 483 791 228
Fragments arranged by size:
791 483 228 163
Bpu10I CC'TnA_GC
Cuts at: 0 1590 1665
Size: 1590 75
BsaAI yAC'GTr
Cuts at: 0 705 1665
Size: 705 960
BsaHI Gr'CG_yC
Cuts at: 0 386 1665
Size: 386 1279
BsaWI w'CCGG_w
Cuts at: 0 847 1445 1665
Size: 847 598 220
Fragments arranged by size:
847 598 220
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 169 199 888 918 936 966 1435 1465
Size: 169 30 689 30 18 30 469 30
Cuts at: 1465 1607 1637 1665
Size: 142 30 28
Fragments arranged by size:
689 469 169 142 30 30 30 30
30 28 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 1031 1422 1578 1665
Size: 160 871 391 156 87
Fragments arranged by size:
871 391 160 156 87
BseYI C'CCAG_C
Cuts at: 0 150 1665
Size: 150 1515
BsiEI CG_ry'CG
Cuts at: 0 1466 1665
Size: 1466 199
BsiHKAI G_wGCw'C
Cuts at: 0 213 380 1432 1665
Size: 213 167 1052 233
Fragments arranged by size:
1052 233 213 167
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1173 1665
Size: 1173 492
Bsp1286I G_dGCh'C
Cuts at: 0 25 213 380 423 1432 1665
Size: 25 188 167 43 1009 233
Fragments arranged by size:
1009 233 188 167 43 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1499 1665
Size: 1499 166
BsrBI CCG'CTC
Cuts at: 0 1404 1470 1665
Size: 1404 66 195
Fragments arranged by size:
1404 195 66
BsrDI GCAATG_nn'
Cuts at: 0 1120 1193 1665
Size: 1120 73 472
Fragments arranged by size:
1120 472 73
BsrFI r'CCGG_y
Cuts at: 0 1385 1445 1665
Size: 1385 60 220
Fragments arranged by size:
1385 220 60
BsrGI T'GTAC_A
Cuts at: 0 674 939 1665
Size: 674 265 726
Fragments arranged by size:
726 674 265
BstAPI GCAn_nnn'nTGC
Cuts at: 0 120 1665
Size: 120 1545
BstXI CCAn_nnnn'nTGG
Cuts at: 0 33 1665
Size: 33 1632
BstYI r'GATC_y
Cuts at: 0 597 742 1026 1527 1665
Size: 597 145 284 501 138
Fragments arranged by size:
597 501 284 145 138
Bsu36I CC'TnA_GG
Cuts at: 0 1133 1665
Size: 1133 532
BtgI C'CryG_G
Cuts at: 0 1285 1665
Size: 1285 380
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 834 869 1274 1309 1665
Size: 834 35 405 35 356
Fragments arranged by size:
834 405 356 35 35
DraI TTT'AAA
Cuts at: 0 560 1665
Size: 560 1105
DrdI GACnn_nn'nnGTC
Cuts at: 0 311 969 1352 1665
Size: 311 658 383 313
Fragments arranged by size:
658 383 313 311
EarI CTCTTCn'nnn_
Cuts at: 0 1418 1665
Size: 1418 247
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1034 1665
Size: 1034 631
EcoICRI GAG'CTC
Cuts at: 0 378 1430 1665
Size: 378 1052 235
Fragments arranged by size:
1052 378 235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 646 1021 1437 1665
Size: 163 483 375 416 228
Fragments arranged by size:
483 416 375 228 163
EcoNI CCTnn'n_nnAGG
Cuts at: 0 659 1665
Size: 659 1006
EcoO109I rG'GnC_Cy
Cuts at: 0 138 345 396 1665
Size: 138 207 51 1269
Fragments arranged by size:
1269 207 138 51
EcoRI G'AATT_C
Cuts at: 0 1152 1665
Size: 1152 513
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 335 528 1665
Size: 335 193 1137
Fragments arranged by size:
1137 335 193
HaeII r_GCGC'y
Cuts at: 0 389 876 1544 1665
Size: 389 487 668 121
Fragments arranged by size:
668 487 389 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 86 117 118 149 825 857 936 968
Size: 86 31 1 31 676 32 79 32
Cuts at: 968 1665
Size: 697
Fragments arranged by size:
697 676 86 79 32 32 31 31
1
HincII GTy'rAC
Cuts at: 0 1658 1665
Size: 1658 7
KasI G'GCGC_C
Cuts at: 0 385 1665
Size: 385 1280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 167 1665
Size: 35 132 1498
Fragments arranged by size:
1498 132 35
MslI CAynn'nnrTG
Cuts at: 0 761 1252 1665
Size: 761 491 413
Fragments arranged by size:
761 491 413
MspA1I CmG'CkG
Cuts at: 0 117 810 1634 1665
Size: 117 693 824 31
Fragments arranged by size:
824 693 117 31
NarI GG'CG_CC
Cuts at: 0 386 1665
Size: 386 1279
NspI r_CATG'y
Cuts at: 0 623 675 1665
Size: 623 52 990
Fragments arranged by size:
990 623 52
PflMI CCAn_nnn'nTGG
Cuts at: 0 1126 1276 1665
Size: 1126 150 389
Fragments arranged by size:
1126 389 150
PfoI T'CCnGG_A
Cuts at: 0 1022 1556 1665
Size: 1022 534 109
Fragments arranged by size:
1022 534 109
PmlI CAC'GTG
Cuts at: 0 705 1665
Size: 705 960
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1607 1639 1665
Size: 1607 32 26
Fragments arranged by size:
1607 32 26
PpuMI rG'GwC_Cy
Cuts at: 0 138 1665
Size: 138 1527
PspXI vC'TCGA_Gb
Cuts at: 0 1349 1665
Size: 1349 316
PstI C_TGCA'G
Cuts at: 0 125 738 1665
Size: 125 613 927
Fragments arranged by size:
927 613 125
PvuII CAG'CTG
Cuts at: 0 117 810 1634 1665
Size: 117 693 824 31
Fragments arranged by size:
824 693 117 31
SacI G_AGCT'C
Cuts at: 0 380 1432 1665
Size: 380 1052 233
Fragments arranged by size:
1052 380 233
SfcI C'TryA_G
Cuts at: 0 121 494 734 1665
Size: 121 373 240 931
Fragments arranged by size:
931 373 240 121
SfoI GGC'GCC
Cuts at: 0 387 1665
Size: 387 1278
SmaI CCC'GGG
Cuts at: 0 1069 1665
Size: 1069 596
SmlI C'TyrA_G
Cuts at: 0 865 1349 1665
Size: 865 484 316
Fragments arranged by size:
865 484 316
StuI AGG'CCT
Cuts at: 0 665 1665
Size: 665 1000
StyI C'CwwG_G
Cuts at: 0 392 1665
Size: 392 1273
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1480 1665
Size: 1480 185
TatI w'GTAC_w
Cuts at: 0 674 939 1665
Size: 674 265 726
Fragments arranged by size:
726 674 265
Tth111I GACn'n_nGTC
Cuts at: 0 769 1665
Size: 769 896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 33 399 1665
Size: 33 366 1266
Fragments arranged by size:
1266 366 33
XhoI C'TCGA_G
Cuts at: 0 1349 1665
Size: 1349 316
XmaI C'CCGG_G
Cuts at: 0 1067 1665
Size: 1067 598
XmnI GAAnn'nnTTC
Cuts at: 0 773 1156 1665
Size: 773 383 509
Fragments arranged by size:
773 509 383
Enzymes that do cut:
AarI AcuI AflII AflIII AgeI AloI AlwNI ApaLI
ApoI AvaI BanI BanII BbeI BbvCI BciVI BglI
BglII Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI
Bsu36I BtgI CspCI DraI DrdI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
KasI MmeI MslI MspA1I NarI NspI PflMI PfoI
PmlI PpiI PpuMI PspXI PstI PvuII SacI SfcI
SfoI SmaI SmlI StuI StyI TaqII TatI Tth111I
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AhdI AleI AlfI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BcgI BclI BfrBI BlpI BmgBI BmtI BpuEI BsaI
BsaBI BsgI BsiWI BsmI BspEI BspHI BssHII BssSI
BstBI BstEII BstZ17I BtgZI BtsI ClaI DraIII EaeI
EagI EcoRV FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI MscI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PshAI
PsiI PspOMI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TstI XbaI ZraI