Restriction map for
pF1KB8006
(Linear) (Six-base) MAPSORT of: KIBB8006.seq Check: 5925 from: 1 to: 2319
KIBB8006.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 315 1254 2319
Size: 315 939 1065
Fragments arranged by size:
1065 939 315
AccI GT'mk_AC
Cuts at: 0 1823 2319
Size: 1823 496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1775 2026 2319
Size: 1775 251 293
Fragments arranged by size:
1775 293 251
AfeI AGC'GCT
Cuts at: 0 86 2319
Size: 86 2233
AflII C'TTAA_G
Cuts at: 0 334 343 2319
Size: 334 9 1976
Fragments arranged by size:
1976 334 9
AflIII A'CryG_T
Cuts at: 0 1907 2058 2319
Size: 1907 151 261
Fragments arranged by size:
1907 261 151
AhdI GACnn_n'nnGTC
Cuts at: 0 819 2319
Size: 819 1500
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 323 2177 2319
Size: 323 1854 142
Fragments arranged by size:
1854 323 142
AlwNI CAG_nnn'CTG
Cuts at: 0 1249 2049 2319
Size: 1249 800 270
Fragments arranged by size:
1249 800 270
ApaI G_GGCC'C
Cuts at: 0 41 1211 1257 2319
Size: 41 1170 46 1062
Fragments arranged by size:
1170 1062 46 41
ApaLI G'TGCA_C
Cuts at: 0 2304 2319
Size: 2304 15
ApoI r'AATT_y
Cuts at: 0 705 1315 1369 2319
Size: 705 610 54 950
Fragments arranged by size:
950 705 610 54
AvaI C'yCGr_G
Cuts at: 0 971 1203 1309 1706 1783 2319
Size: 971 232 106 397 77 536
Fragments arranged by size:
971 536 397 232 106 77
BamHI G'GATC_C
Cuts at: 0 232 2319
Size: 232 2087
BanI G'GyrC_C
Cuts at: 0 1627 2319
Size: 1627 692
BanII G_rGCy'C
Cuts at: 0 19 41 72 1211 1257 2300 2319
Size: 19 22 31 1139 46 1043 19
Fragments arranged by size:
1139 1043 46 31 22 19 19
BbvCI CC'TCA_GC
Cuts at: 0 188 1238 2194 2228 2319
Size: 188 1050 956 34 91
Fragments arranged by size:
1050 956 188 91 34
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 166 200 724 758 1384 1418 2319
Size: 166 34 524 34 626 34 901
Fragments arranged by size:
901 626 524 166 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1252 2319
Size: 1252 1067
BlpI GC'TnA_GC
Cuts at: 0 1385 2319
Size: 1385 934
Bme1580I G_kGCm'C
Cuts at: 0 41 771 1211 1257 2308 2319
Size: 41 730 440 46 1051 11
Fragments arranged by size:
1051 730 440 46 41 11
BmgBI CAC'GTC
Cuts at: 0 429 474 1910 2316 2319
Size: 429 45 1436 406 3
Fragments arranged by size:
1436 429 406 45 3
BmrI ACTGGGnnnn_n'
Cuts at: 0 2079 2319
Size: 2079 240
BmtI G_CTAG'C
Cuts at: 0 2227 2319
Size: 2227 92
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 36 38 86 1203 2319
Size: 36 2 48 1117 1116
Fragments arranged by size:
1117 1116 48 36 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 962 1183 1351 2319
Size: 962 221 168 968
Fragments arranged by size:
968 962 221 168
Bpu10I CC'TnA_GC
Cuts at: 0 188 454 1144 1238 2194 2228 2319
Size: 188 266 690 94 956 34 91
Fragments arranged by size:
956 690 266 188 94 91 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1555 2319
Size: 1555 764
BsaHI Gr'CG_yC
Cuts at: 0 2275 2319
Size: 2275 44
BsaWI w'CCGG_w
Cuts at: 0 314 2319
Size: 314 2005
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 38 68 489 519 935 965 2319
Size: 38 30 421 30 416 30 1354
Fragments arranged by size:
1354 421 416 38 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 244 565 1025 2224 2254 2319
Size: 244 321 460 1199 30 65
Fragments arranged by size:
1199 460 321 244 65 30
BseYI C'CCAG_C
Cuts at: 0 1843 2282 2319
Size: 1843 439 37
Fragments arranged by size:
1843 439 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 862 1531 2035 2170 2319
Size: 155 707 669 504 135 149
Fragments arranged by size:
707 669 504 155 149 135
BsiHKAI G_wGCw'C
Cuts at: 0 72 596 875 2308 2319
Size: 72 524 279 1433 11
Fragments arranged by size:
1433 524 279 72 11
Bsp1286I G_dGCh'C
Cuts at: 0 19 41 72 596 771 875 1211 1257
Size: 19 22 31 524 175 104 336 46
Cuts at: 1257 2300 2308 2319
Size: 1043 8 11
Fragments arranged by size:
1043 524 336 175 104 46 31 22
19 11 8
BspEI T'CCGG_A
Cuts at: 0 314 2319
Size: 314 2005
BspHI T'CATG_A
Cuts at: 0 923 2319
Size: 923 1396
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 315 1254 2192 2319
Size: 315 939 938 127
Fragments arranged by size:
939 938 315 127
BsrBI CCG'CTC
Cuts at: 0 27 1389 2319
Size: 27 1362 930
Fragments arranged by size:
1362 930 27
BsrGI T'GTAC_A
Cuts at: 0 407 2319
Size: 407 1912
BssSI C'ACGA_G
Cuts at: 0 1549 1873 2319
Size: 1549 324 446
Fragments arranged by size:
1549 446 324
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1983 2319
Size: 1983 336
BstBI TT'CG_AA
Cuts at: 0 1320 2319
Size: 1320 999
BstEII G'GTnAC_C
Cuts at: 0 978 1635 2319
Size: 978 657 684
Fragments arranged by size:
978 684 657
BstXI CCAn_nnnn'nTGG
Cuts at: 0 940 2319
Size: 940 1379
BstYI r'GATC_y
Cuts at: 0 232 1660 1865 2036 2319
Size: 232 1428 205 171 283
Fragments arranged by size:
1428 283 232 205 171
BtgI C'CryG_G
Cuts at: 0 1893 2309 2319
Size: 1893 416 10
Fragments arranged by size:
1893 416 10
BtsI GCAGTG_nn'
Cuts at: 0 596 1107 2319
Size: 596 511 1212
Fragments arranged by size:
1212 596 511
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 864 899 2319
Size: 864 35 1420
Fragments arranged by size:
1420 864 35
DraIII CAC_nnn'GTG
Cuts at: 0 1983 2319
Size: 1983 336
DrdI GACnn_nn'nnGTC
Cuts at: 0 1819 2319
Size: 1819 500
EaeI y'GGCC_r
Cuts at: 0 276 885 2094 2319
Size: 276 609 1209 225
Fragments arranged by size:
1209 609 276 225
EarI CTCTTCn'nnn_
Cuts at: 0 224 689 1751 1942 2212 2319
Size: 224 465 1062 191 270 107
Fragments arranged by size:
1062 465 270 224 191 107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 451 818 1441 1567 2268 2319
Size: 451 367 623 126 701 51
Fragments arranged by size:
701 623 451 367 126 51
EcoICRI GAG'CTC
Cuts at: 0 70 2319
Size: 70 2249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 962 1183 1351 1775 2026 2319
Size: 962 221 168 424 251 293
Fragments arranged by size:
962 424 293 251 221 168
EcoNI CCTnn'n_nnAGG
Cuts at: 0 987 1261 2319
Size: 987 274 1058
Fragments arranged by size:
1058 987 274
EcoO109I rG'GnC_Cy
Cuts at: 0 192 1207 1208 1253 1254 2319
Size: 192 1015 1 45 1 1065
Fragments arranged by size:
1065 1015 192 45 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 778 1637 2319
Size: 778 859 682
Fragments arranged by size:
859 778 682
FspI TGC'GCA
Cuts at: 0 2086 2319
Size: 2086 233
HaeII r_GCGC'y
Cuts at: 0 88 2319
Size: 88 2231
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 139 157 171 189 805 837 2319
Size: 139 18 14 18 616 32 1482
Fragments arranged by size:
1482 616 139 32 18 18 14
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1992 2319
Size: 1992 327
MscI TGG'CCA
Cuts at: 0 278 2319
Size: 278 2041
MslI CAynn'nnrTG
Cuts at: 0 2057 2319
Size: 2057 262
MspA1I CmG'CkG
Cuts at: 0 906 2046 2319
Size: 906 1140 273
Fragments arranged by size:
1140 906 273
NcoI C'CATG_G
Cuts at: 0 1893 2319
Size: 1893 426
NheI G'CTAG_C
Cuts at: 0 2223 2319
Size: 2223 96
NspI r_CATG'y
Cuts at: 0 481 1560 2062 2091 2319
Size: 481 1079 502 29 228
Fragments arranged by size:
1079 502 481 228 29
PasI CC'CwG_GG
Cuts at: 0 1249 1257 2319
Size: 1249 8 1062
Fragments arranged by size:
1249 1062 8
PciI A'CATG_T
Cuts at: 0 2058 2319
Size: 2058 261
PflMI CCAn_nnn'nTGG
Cuts at: 0 1899 2319
Size: 1899 420
PpuMI rG'GwC_Cy
Cuts at: 0 192 2319
Size: 192 2127
PsiI TTA'TAA
Cuts at: 0 1064 2319
Size: 1064 1255
PspOMI G'GGCC_C
Cuts at: 0 37 1207 1253 2319
Size: 37 1170 46 1066
Fragments arranged by size:
1170 1066 46 37
PspXI vC'TCGA_Gb
Cuts at: 0 1783 2319
Size: 1783 536
PstI C_TGCA'G
Cuts at: 0 385 1158 2156 2319
Size: 385 773 998 163
Fragments arranged by size:
998 773 385 163
PvuII CAG'CTG
Cuts at: 0 2046 2319
Size: 2046 273
RsrII CG'GwC_CG
Cuts at: 0 312 2319
Size: 312 2007
SacI G_AGCT'C
Cuts at: 0 72 2319
Size: 72 2247
SexAI A'CCwGG_T
Cuts at: 0 982 2319
Size: 982 1337
SfcI C'TryA_G
Cuts at: 0 270 381 896 1154 2152 2319
Size: 270 111 515 258 998 167
Fragments arranged by size:
998 515 270 258 167 111
SmaI CCC'GGG
Cuts at: 0 1205 2319
Size: 1205 1114
SmlI C'TyrA_G
Cuts at: 0 334 343 1309 1570 1783 2319
Size: 334 9 966 261 213 536
Fragments arranged by size:
966 536 334 261 213 9
SphI G_CATG'C
Cuts at: 0 481 2319
Size: 481 1838
StuI AGG'CCT
Cuts at: 0 700 761 2319
Size: 700 61 1558
Fragments arranged by size:
1558 700 61
StyI C'CwwG_G
Cuts at: 0 1893 2319
Size: 1893 426
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1183 1506 1891 2069 2319
Size: 1183 323 385 178 250
Fragments arranged by size:
1183 385 323 250 178
TatI w'GTAC_w
Cuts at: 0 407 1553 2319
Size: 407 1146 766
Fragments arranged by size:
1146 766 407
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 38 70 296 328 489 521 935 967
Size: 38 32 226 32 161 32 414 32
Cuts at: 967 1706 1738 2021 2053 2319
Size: 739 32 283 32 266
Fragments arranged by size:
739 414 283 266 226 161 38 32
32 32 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 823 2319
Size: 823 1496
XhoI C'TCGA_G
Cuts at: 0 1309 1783 2319
Size: 1309 474 536
Fragments arranged by size:
1309 536 474
XmaI C'CCGG_G
Cuts at: 0 1203 2319
Size: 1203 1116
XmnI GAAnn'nnTTC
Cuts at: 0 1369 1928 2319
Size: 1369 559 391
Fragments arranged by size:
1369 559 391
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AhdI AlfI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I MmeI
MscI MslI MspA1I NcoI NheI NspI PasI PciI
PflMI PpuMI PsiI PspOMI PspXI PstI PvuII RsrII
SacI SexAI SfcI SmaI SmlI SphI StuI StyI
TaqII TatI TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AgeI AleI AloI AscI AseI
AsiSI AvrII BaeI BbeI BbsI BciVI BclI BfrBI
BglII BsaI BsaAI BsaBI BsiEI BsiWI BsmI BsmBI
BsrDI BsrFI BssHII BstZ17I Bsu36I BtgZI ClaI DraI
EagI EcoRI EcoRV FseI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NotI NruI NsiI PacI PfoI PmeI PmlI PpiI
PshAI PsrI PvuI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI ZraI