Restriction map for
pF1KB8005
(Linear) (Six-base) MAPSORT of: KIBB8005.seq Check: 8697 from: 1 to: 2244
KIBB8005.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 653 1861 1999 2244
Size: 391 262 1208 138 245
Fragments arranged by size:
1208 391 262 245 138
AflIII A'CryG_T
Cuts at: 0 655 2224 2244
Size: 655 1569 20
Fragments arranged by size:
1569 655 20
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1191 2090 2244
Size: 1191 899 154
Fragments arranged by size:
1191 899 154
AlwNI CAG_nnn'CTG
Cuts at: 0 154 2244
Size: 154 2090
ApaI G_GGCC'C
Cuts at: 0 32 2244
Size: 32 2212
ApoI r'AATT_y
Cuts at: 0 1130 1273 2244
Size: 1130 143 971
Fragments arranged by size:
1130 971 143
AseI AT'TA_AT
Cuts at: 0 1264 2244
Size: 1264 980
AvaI C'yCGr_G
Cuts at: 0 2036 2082 2244
Size: 2036 46 162
Fragments arranged by size:
2036 162 46
BamHI G'GATC_C
Cuts at: 0 2029 2244
Size: 2029 215
BanII G_rGCy'C
Cuts at: 0 32 1871 2244
Size: 32 1839 373
Fragments arranged by size:
1839 373 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 286 397 659 1849 2244
Size: 286 111 262 1190 395
Fragments arranged by size:
1190 395 286 262 111
BciVI GTATCCnnnnn_n'
Cuts at: 0 1598 1720 2244
Size: 1598 122 524
Fragments arranged by size:
1598 524 122
BfrBI ATG'CAT
Cuts at: 0 1663 2244
Size: 1663 581
BglII A'GATC_T
Cuts at: 0 1248 2244
Size: 1248 996
Bme1580I G_kGCm'C
Cuts at: 0 32 854 2244
Size: 32 822 1390
Fragments arranged by size:
1390 822 32
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 734 1782 2244
Size: 734 1048 462
Fragments arranged by size:
1048 734 462
Bpu10I CC'TnA_GC
Cuts at: 0 110 2244
Size: 110 2134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1630 2244
Size: 1630 614
BsaI GGTCTCn'nnnn_
Cuts at: 0 410 1103 1233 2244
Size: 410 693 130 1011
Fragments arranged by size:
1011 693 410 130
BsaBI GATnn'nnATC
Cuts at: 0 1580 2244
Size: 1580 664
BsaWI w'CCGG_w
Cuts at: 0 1428 1578 2244
Size: 1428 150 666
Fragments arranged by size:
1428 666 150
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 425 455 2244
Size: 425 30 1789
Fragments arranged by size:
1789 425 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 705 737 2244
Size: 705 32 1507
Fragments arranged by size:
1507 705 32
BseYI C'CCAG_C
Cuts at: 0 485 2244
Size: 485 1759
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2100 2244
Size: 2100 144
BsiEI CG_ry'CG
Cuts at: 0 571 2244
Size: 571 1673
BsiHKAI G_wGCw'C
Cuts at: 0 832 1871 2244
Size: 832 1039 373
Fragments arranged by size:
1039 832 373
BsmI GAATG_Cn'
Cuts at: 0 1087 2244
Size: 1087 1157
Bsp1286I G_dGCh'C
Cuts at: 0 32 832 854 1871 2244
Size: 32 800 22 1017 373
Fragments arranged by size:
1017 800 373 32 22
BspEI T'CCGG_A
Cuts at: 0 1428 1578 2244
Size: 1428 150 666
Fragments arranged by size:
1428 666 150
BspHI T'CATG_A
Cuts at: 0 1229 2244
Size: 1229 1015
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1159 2135 2244
Size: 1159 976 109
Fragments arranged by size:
1159 976 109
BsrDI GCAATG_nn'
Cuts at: 0 142 556 892 1173 1396 1513 2062 2244
Size: 142 414 336 281 223 117 549 182
Fragments arranged by size:
549 414 336 281 223 182 142 117
BsrFI r'CCGG_y
Cuts at: 0 570 2244
Size: 570 1674
BsrGI T'GTAC_A
Cuts at: 0 1936 2244
Size: 1936 308
BstXI CCAn_nnnn'nTGG
Cuts at: 0 485 1456 2244
Size: 485 971 788
Fragments arranged by size:
971 788 485
BstYI r'GATC_y
Cuts at: 0 127 251 672 858 911 978 1248 1575
Size: 127 124 421 186 53 67 270 327
Cuts at: 1575 1581 2029 2244
Size: 6 448 215
Fragments arranged by size:
448 421 327 270 215 186 127 124
67 53 6
Bsu36I CC'TnA_GG
Cuts at: 0 1163 2244
Size: 1163 1081
DraI TTT'AAA
Cuts at: 0 1013 2244
Size: 1013 1231
EarI CTCTTCn'nnn_
Cuts at: 0 875 1193 1372 1988 2244
Size: 875 318 179 616 256
Fragments arranged by size:
875 616 318 256 179
EcoICRI GAG'CTC
Cuts at: 0 1869 2244
Size: 1869 375
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 653 734 1782 1861 1999 2244
Size: 391 262 81 1048 79 138 245
Fragments arranged by size:
1048 391 262 245 138 81 79
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1166 2244
Size: 1166 1078
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1309 2244
Size: 1309 935
HaeII r_GCGC'y
Cuts at: 0 561 2244
Size: 561 1683
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 896 928 2244
Size: 896 32 1316
Fragments arranged by size:
1316 896 32
HincII GTy'rAC
Cuts at: 0 948 2244
Size: 948 1296
MfeI C'AATT_G
Cuts at: 0 873 1027 1531 2244
Size: 873 154 504 713
Fragments arranged by size:
873 713 504 154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 323 1437 2244
Size: 114 209 1114 807
Fragments arranged by size:
1114 807 209 114
MslI CAynn'nnrTG
Cuts at: 0 1234 2244
Size: 1234 1010
NdeI CA'TA_TG
Cuts at: 0 1665 2244
Size: 1665 579
NsiI A_TGCA'T
Cuts at: 0 1665 2244
Size: 1665 579
NspI r_CATG'y
Cuts at: 0 659 2228 2244
Size: 659 1569 16
Fragments arranged by size:
1569 659 16
PasI CC'CwG_GG
Cuts at: 0 854 2244
Size: 854 1390
PciI A'CATG_T
Cuts at: 0 655 2224 2244
Size: 655 1569 20
Fragments arranged by size:
1569 655 20
PflMI CCAn_nnn'nTGG
Cuts at: 0 637 1319 1360 2244
Size: 637 682 41 884
Fragments arranged by size:
884 682 637 41
PfoI T'CCnGG_A
Cuts at: 0 900 2244
Size: 900 1344
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1100 1132 2244
Size: 1100 32 1112
Fragments arranged by size:
1112 1100 32
PshAI GACnn'nnGTC
Cuts at: 0 452 2244
Size: 452 1792
PsiI TTA'TAA
Cuts at: 0 1526 2244
Size: 1526 718
PspOMI G'GGCC_C
Cuts at: 0 28 2244
Size: 28 2216
PstI C_TGCA'G
Cuts at: 0 454 1422 1805 2051 2079 2244
Size: 454 968 383 246 28 165
Fragments arranged by size:
968 454 383 246 165 28
SacI G_AGCT'C
Cuts at: 0 1871 2244
Size: 1871 373
SapI GCTCTTCn'nnn_
Cuts at: 0 875 1988 2244
Size: 875 1113 256
Fragments arranged by size:
1113 875 256
ScaI AGT'ACT
Cuts at: 0 53 2244
Size: 53 2191
SexAI A'CCwGG_T
Cuts at: 0 68 1112 2244
Size: 68 1044 1132
Fragments arranged by size:
1132 1044 68
SfcI C'TryA_G
Cuts at: 0 146 450 1418 1801 2047 2075 2244
Size: 146 304 968 383 246 28 169
Fragments arranged by size:
968 383 304 246 169 146 28
SmlI C'TyrA_G
Cuts at: 0 1609 2036 2244
Size: 1609 427 208
Fragments arranged by size:
1609 427 208
SspI AAT'ATT
Cuts at: 0 583 2244
Size: 583 1661
StuI AGG'CCT
Cuts at: 0 1292 2244
Size: 1292 952
StyI C'CwwG_G
Cuts at: 0 1096 1553 2244
Size: 1096 457 691
Fragments arranged by size:
1096 691 457
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 434 2244
Size: 434 1810
TatI w'GTAC_w
Cuts at: 0 51 1936 2244
Size: 51 1885 308
Fragments arranged by size:
1885 308 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 428 460 1463 1495 2222 2244
Size: 428 32 1003 32 727 22
Fragments arranged by size:
1003 727 428 32 32 22
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1250 2244
Size: 1250 994
XhoI C'TCGA_G
Cuts at: 0 2036 2244
Size: 2036 208
XmnI GAAnn'nnTTC
Cuts at: 0 1676 1823 2244
Size: 1676 147 421
Fragments arranged by size:
1676 421 147
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaI ApoI AseI AvaI
BamHI BanII BbsI BciVI BfrBI BglII Bme1580I BpmI
Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BsrGI BstXI BstYI Bsu36I DraI EarI
EcoICRI Eco57MI EcoNI FalI HaeII Hin4I HincII MfeI
MmeI MslI NdeI NsiI NspI PasI PciI PflMI
PfoI PpiI PshAI PsiI PspOMI PstI SacI SapI
ScaI SexAI SfcI SmlI SspI StuI StyI TaqII
TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaLI AscI AsiSI AvrII BaeI
BanI BbeI BbvCI BcgI BclI BglI BlpI BmgBI
BmrI BmtI BplI BsaAI BsaHI BsiWI BsmBI BsrBI
BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoO109I EcoRI EcoRV FseI FspI FspAI HindIII HpaI
KasI KpnI MluI MscI MspA1I NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI PmeI PmlI PpuMI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI Tth111I XbaI XmaI ZraI