(Linear) (Six-base) MAPSORT of: KIBB8002.seq  Check: 5470  from: 1  to: 1128



KIBB8002.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     479     956    1128 
   Size:        479     477     172

  Fragments arranged by size: 

                 479     477     172
 
AflII C'TTAA_G

Cuts at:      0     862    1128 
   Size:        862     266
 
AleI CACnn'nnGTG

Cuts at:      0     602    1128 
   Size:        602     526
 
AlwNI CAG_nnn'CTG

Cuts at:      0     979    1128 
   Size:        979     149
 
ApoI r'AATT_y

Cuts at:      0      31      82     263     633     825    1101    1128 
   Size:         31      51     181     370     192     276      27

  Fragments arranged by size: 

                 370     276     192     181      51      31      27
 
BanI G'GyrC_C

Cuts at:      0     643    1128 
   Size:        643     485
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     502    1056    1128 
   Size:        502     554      72

  Fragments arranged by size: 

                 554     502      72
 
BclI T'GATC_A

Cuts at:      0    1122    1128 
   Size:       1122       6
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1054    1128 
   Size:       1054      74
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     723    1128 
   Size:        723     405
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     937    1128 
   Size:        937     191
 
BspHI T'CATG_A

Cuts at:      0      87     918    1128 
   Size:         87     831     210

  Fragments arranged by size: 

                 831     210      87
 
BsrDI GCAATG_nn'

Cuts at:      0     206    1128 
   Size:        206     922
 
BstEII G'GTnAC_C

Cuts at:      0     406    1128 
   Size:        406     722
 
BstYI r'GATC_y

Cuts at:      0     363    1128 
   Size:        363     765
 
BtsI GCAGTG_nn'

Cuts at:      0     497    1128 
   Size:        497     631
 
DraI TTT'AAA

Cuts at:      0    1011    1128 
   Size:       1011     117
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    1004    1128 
   Size:         19     985     124

  Fragments arranged by size: 

                 985     124      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     479     956    1128 
   Size:        479     477     172

  Fragments arranged by size: 

                 479     477     172
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     157    1128 
   Size:        157     971
 
EcoRI G'AATT_C

Cuts at:      0     825    1128 
   Size:        825     303
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     306     338     415     447     898     930     949     981 
   Size:        306      32      77      32     451      32      19      32

Cuts at:    981    1128 
   Size:        147

  Fragments arranged by size: 

                 451     306     147      77      32      32      32      32
                  19
 
MslI CAynn'nnrTG

Cuts at:      0     444     602    1128 
   Size:        444     158     526

  Fragments arranged by size: 

                 526     444     158
 
MspA1I CmG'CkG

Cuts at:      0     211    1128 
   Size:        211     917
 
NdeI CA'TA_TG

Cuts at:      0     879    1128 
   Size:        879     249
 
PvuII CAG'CTG

Cuts at:      0     211    1128 
   Size:        211     917
 
SfcI C'TryA_G

Cuts at:      0     722    1128 
   Size:        722     406
 
SmlI C'TyrA_G

Cuts at:      0     862    1128 
   Size:        862     266
 
SpeI A'CTAG_T

Cuts at:      0     601    1128 
   Size:        601     527
 
StuI AGG'CCT

Cuts at:      0      72    1128 
   Size:         72    1056
 
XbaI T'CTAG_A

Cuts at:      0    1096    1128 
   Size:       1096      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     884    1128 
   Size:        884     244
 
XmnI GAAnn'nnTTC

Cuts at:      0     464    1128 
   Size:        464     664
 

 Enzymes that do cut:

     AcuI    AflII     AleI    AlwNI     ApoI     BanI     BbsI     BclI
     BmrI     BsaI    BseRI    BspHI    BsrDI   BstEII    BstYI     BtsI
     DraI     EciI  Eco57MI    EcoNI    EcoRI    Hin4I     MslI   MspA1I
     NdeI    PvuII     SfcI     SmlI     SpeI     StuI     XbaI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI
     SrfI     SspI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XhoI     XmaI     ZraI