Restriction map for
pF1KB8001
(Linear) (Six-base) MAPSORT of: KIBB8001.seq Check: 309 from: 1 to: 795
KIBB8001.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AccI GT'mk_AC
Cuts at: 0 446 795
Size: 446 349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 795
Size: 317 478
AlwNI CAG_nnn'CTG
Cuts at: 0 295 706 748 795
Size: 295 411 42 47
Fragments arranged by size:
411 295 47 42
ApaI G_GGCC'C
Cuts at: 0 713 795
Size: 713 82
AvaI C'yCGr_G
Cuts at: 0 638 795
Size: 638 157
BanI G'GyrC_C
Cuts at: 0 527 795
Size: 527 268
BanII G_rGCy'C
Cuts at: 0 185 713 795
Size: 185 528 82
Fragments arranged by size:
528 185 82
BbeI G_GCGC'C
Cuts at: 0 531 795
Size: 531 264
BciVI GTATCCnnnnn_n'
Cuts at: 0 228 743 795
Size: 228 515 52
Fragments arranged by size:
515 228 52
Bme1580I G_kGCm'C
Cuts at: 0 646 713 795
Size: 646 67 82
Fragments arranged by size:
646 82 67
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 64 795
Size: 64 731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 795
Size: 79 716
BsaAI yAC'GTr
Cuts at: 0 647 795
Size: 647 148
BsaHI Gr'CG_yC
Cuts at: 0 528 632 795
Size: 528 104 163
Fragments arranged by size:
528 163 104
BsaWI w'CCGG_w
Cuts at: 0 311 795
Size: 311 484
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 229 259 795
Size: 229 30 536
Fragments arranged by size:
536 229 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 559 795
Size: 559 236
BseYI C'CCAG_C
Cuts at: 0 706 795
Size: 706 89
BsiEI CG_ry'CG
Cuts at: 0 730 795
Size: 730 65
BsiHKAI G_wGCw'C
Cuts at: 0 755 795
Size: 755 40
BsmI GAATG_Cn'
Cuts at: 0 204 795
Size: 204 591
Bsp1286I G_dGCh'C
Cuts at: 0 185 646 713 755 795
Size: 185 461 67 42 40
Fragments arranged by size:
461 185 67 42 40
BsrDI GCAATG_nn'
Cuts at: 0 83 795
Size: 83 712
BstEII G'GTnAC_C
Cuts at: 0 252 795
Size: 252 543
BstXI CCAn_nnnn'nTGG
Cuts at: 0 132 152 249 795
Size: 132 20 97 546
Fragments arranged by size:
546 132 97 20
BtgI C'CryG_G
Cuts at: 0 248 486 608 795
Size: 248 238 122 187
Fragments arranged by size:
248 238 187 122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 520 795
Size: 520 275
EaeI y'GGCC_r
Cuts at: 0 32 263 727 795
Size: 32 231 464 68
Fragments arranged by size:
464 231 68 32
EagI C'GGCC_G
Cuts at: 0 727 795
Size: 727 68
EarI CTCTTCn'nnn_
Cuts at: 0 71 506 590 721 795
Size: 71 435 84 131 74
Fragments arranged by size:
435 131 84 74 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 317 795
Size: 79 238 478
Fragments arranged by size:
478 238 79
EcoO109I rG'GnC_Cy
Cuts at: 0 138 710 795
Size: 138 572 85
Fragments arranged by size:
572 138 85
HaeII r_GCGC'y
Cuts at: 0 531 692 795
Size: 531 161 103
Fragments arranged by size:
531 161 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 202 229 234 261 604 636 795
Size: 202 27 5 27 343 32 159
Fragments arranged by size:
343 202 159 32 27 27 5
HincII GTy'rAC
Cuts at: 0 653 795
Size: 653 142
KasI G'GCGC_C
Cuts at: 0 527 795
Size: 527 268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 287 308 329 795
Size: 113 174 21 21 466
Fragments arranged by size:
466 174 113 21 21
MscI TGG'CCA
Cuts at: 0 34 795
Size: 34 761
MspA1I CmG'CkG
Cuts at: 0 6 488 706 726 795
Size: 6 482 218 20 69
Fragments arranged by size:
482 218 69 20 6
NarI GG'CG_CC
Cuts at: 0 528 795
Size: 528 267
NcoI C'CATG_G
Cuts at: 0 608 795
Size: 608 187
PasI CC'CwG_GG
Cuts at: 0 742 795
Size: 742 53
PmlI CAC'GTG
Cuts at: 0 647 795
Size: 647 148
PspOMI G'GGCC_C
Cuts at: 0 709 795
Size: 709 86
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 148 180 795
Size: 148 32 615
Fragments arranged by size:
615 148 32
PstI C_TGCA'G
Cuts at: 0 23 795
Size: 23 772
PvuII CAG'CTG
Cuts at: 0 706 795
Size: 706 89
RsrII CG'GwC_CG
Cuts at: 0 351 795
Size: 351 444
SacII CC_GC'GG
Cuts at: 0 489 795
Size: 489 306
SexAI A'CCwGG_T
Cuts at: 0 127 795
Size: 127 668
SfcI C'TryA_G
Cuts at: 0 19 795
Size: 19 776
SfoI GGC'GCC
Cuts at: 0 529 795
Size: 529 266
SmaI CCC'GGG
Cuts at: 0 640 795
Size: 640 155
StuI AGG'CCT
Cuts at: 0 772 795
Size: 772 23
StyI C'CwwG_G
Cuts at: 0 608 795
Size: 608 187
XmaI C'CCGG_G
Cuts at: 0 638 795
Size: 638 157
Enzymes that do cut:
AccI AcuI AlwNI ApaI AvaI BanI BanII BbeI
BciVI Bme1580I BplI BpmI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BsrDI BstEII
BstXI BtgI BtgZI EaeI EagI EarI Eco57MI EcoO109I
HaeII Hin4I HincII KasI MmeI MscI MspA1I NarI
NcoI PasI PmlI PspOMI PsrI PstI PvuII RsrII
SacII SexAI SfcI SfoI SmaI StuI StyI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI Bpu10I
BpuEI BsaI BsaBI BsgI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII
DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI MslI
NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PvuI SacI SalI SanDI SapI SbfI
ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmnI ZraI