Restriction map for
pF1KB7996
(Linear) (Six-base) MAPSORT of: KIBB7996.seq Check: 6023 from: 1 to: 1410
KIBB7996.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 486 708 729 738 1410
Size: 486 222 21 9 672
Fragments arranged by size:
672 486 222 21 9
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 1410
Size: 18 1392
AfeI AGC'GCT
Cuts at: 0 791 1410
Size: 791 619
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 148 1410
Size: 148 1262
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1021 1053 1410
Size: 1021 32 357
Fragments arranged by size:
1021 357 32
AlwNI CAG_nnn'CTG
Cuts at: 0 300 441 1410
Size: 300 141 969
Fragments arranged by size:
969 300 141
AvaI C'yCGr_G
Cuts at: 0 85 150 188 395 775 1081 1410
Size: 85 65 38 207 380 306 329
Fragments arranged by size:
380 329 306 207 85 65 38
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 39 72 1410
Size: 39 33 1338
Fragments arranged by size:
1338 39 33
BanI G'GyrC_C
Cuts at: 0 202 1121 1410
Size: 202 919 289
Fragments arranged by size:
919 289 202
BanII G_rGCy'C
Cuts at: 0 142 157 311 1145 1410
Size: 142 15 154 834 265
Fragments arranged by size:
834 265 154 142 15
BbeI G_GCGC'C
Cuts at: 0 206 1410
Size: 206 1204
BbsI GAAGACnn'nnnn_
Cuts at: 0 1312 1385 1410
Size: 1312 73 25
Fragments arranged by size:
1312 73 25
BbvCI CC'TCA_GC
Cuts at: 0 793 1410
Size: 793 617
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 819 853 1410
Size: 819 34 557
Fragments arranged by size:
819 557 34
BclI T'GATC_A
Cuts at: 0 1316 1410
Size: 1316 94
BglI GCCn_nnn'nGGC
Cuts at: 0 137 1048 1410
Size: 137 911 362
Fragments arranged by size:
911 362 137
BlpI GC'TnA_GC
Cuts at: 0 385 1410
Size: 385 1025
Bme1580I G_kGCm'C
Cuts at: 0 1229 1410
Size: 1229 181
BmgBI CAC'GTC
Cuts at: 0 609 1410
Size: 609 801
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 373 484 1048 1066 1219 1410
Size: 105 268 111 564 18 153 191
Fragments arranged by size:
564 268 191 153 111 105 18
Bpu10I CC'TnA_GC
Cuts at: 0 793 1009 1410
Size: 793 216 401
Fragments arranged by size:
793 401 216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 667 1410
Size: 520 147 743
Fragments arranged by size:
743 520 147
BsaI GGTCTCn'nnnn_
Cuts at: 0 704 1324 1410
Size: 704 620 86
Fragments arranged by size:
704 620 86
BsaHI Gr'CG_yC
Cuts at: 0 203 948 1410
Size: 203 745 462
Fragments arranged by size:
745 462 203
BsaWI w'CCGG_w
Cuts at: 0 896 1410
Size: 896 514
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 841 871 1021 1051 1410
Size: 841 30 150 30 359
Fragments arranged by size:
841 359 150 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 147 565 568 712 715 865 1045 1099
Size: 147 418 3 144 3 150 180 54
Cuts at: 1099 1102 1123 1135 1222 1410
Size: 3 21 12 87 188
Fragments arranged by size:
418 188 180 150 147 144 87 54
21 12 3 3 3
BseYI C'CCAG_C
Cuts at: 0 135 1410
Size: 135 1275
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 676 763 1410
Size: 514 162 87 647
Fragments arranged by size:
647 514 162 87
BsiEI CG_ry'CG
Cuts at: 0 318 1410
Size: 318 1092
BsiHKAI G_wGCw'C
Cuts at: 0 157 311 584 596 1145 1410
Size: 157 154 273 12 549 265
Fragments arranged by size:
549 273 265 157 154 12
BsmBI CGTCTCn'nnnn_
Cuts at: 0 550 1410
Size: 550 860
Bsp1286I G_dGCh'C
Cuts at: 0 142 157 311 584 596 1145 1229 1410
Size: 142 15 154 273 12 549 84 181
Fragments arranged by size:
549 273 181 154 142 84 15 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 486 708 722 729 738 845 1410
Size: 486 222 14 7 9 107 565
Fragments arranged by size:
565 486 222 107 14 9 7
BsrBI CCG'CTC
Cuts at: 0 193 199 1410
Size: 193 6 1211
Fragments arranged by size:
1211 193 6
BsrFI r'CCGG_y
Cuts at: 0 28 408 1410
Size: 28 380 1002
Fragments arranged by size:
1002 380 28
BssHII G'CGCG_C
Cuts at: 0 412 414 806 1410
Size: 412 2 392 604
Fragments arranged by size:
604 412 392 2
BssSI C'ACGA_G
Cuts at: 0 589 694 904 1410
Size: 589 105 210 506
Fragments arranged by size:
589 506 210 105
BstAPI GCAn_nnn'nTGC
Cuts at: 0 300 441 1410
Size: 300 141 969
Fragments arranged by size:
969 300 141
BstYI r'GATC_y
Cuts at: 0 953 1217 1410
Size: 953 264 193
Fragments arranged by size:
953 264 193
Bsu36I CC'TnA_GG
Cuts at: 0 1126 1271 1410
Size: 1126 145 139
Fragments arranged by size:
1126 145 139
BtgI C'CryG_G
Cuts at: 0 242 764 1410
Size: 242 522 646
Fragments arranged by size:
646 522 242
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 807 1410
Size: 807 603
BtsI GCAGTG_nn'
Cuts at: 0 1383 1410
Size: 1383 27
EaeI y'GGCC_r
Cuts at: 0 245 272 1410
Size: 245 27 1138
Fragments arranged by size:
1138 245 27
EarI CTCTTCn'nnn_
Cuts at: 0 601 1410
Size: 601 809
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 532 862 1410
Size: 532 330 548
Fragments arranged by size:
548 532 330
EcoICRI GAG'CTC
Cuts at: 0 155 309 1143 1410
Size: 155 154 834 267
Fragments arranged by size:
834 267 155 154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 105 373 484 1048 1066 1219 1410
Size: 18 87 268 111 564 18 153 191
Fragments arranged by size:
564 268 191 153 111 87 18 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 259 841 916 1410
Size: 259 582 75 494
Fragments arranged by size:
582 494 259 75
EcoO109I rG'GnC_Cy
Cuts at: 0 89 252 522 909 1410
Size: 89 163 270 387 501
Fragments arranged by size:
501 387 270 163 89
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 363 1410
Size: 363 1047
HaeII r_GCGC'y
Cuts at: 0 206 231 535 793 1006 1073 1410
Size: 206 25 304 258 213 67 337
Fragments arranged by size:
337 304 258 213 206 67 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 642 674 938 945 970 977 1410
Size: 642 32 264 7 25 7 433
Fragments arranged by size:
642 433 264 32 25 7 7
KasI G'GCGC_C
Cuts at: 0 202 1410
Size: 202 1208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 908 1410
Size: 527 381 502
Fragments arranged by size:
527 502 381
MscI TGG'CCA
Cuts at: 0 274 1410
Size: 274 1136
MspA1I CmG'CkG
Cuts at: 0 426 1410
Size: 426 984
NaeI GCC'GGC
Cuts at: 0 30 410 1410
Size: 30 380 1000
Fragments arranged by size:
1000 380 30
NarI GG'CG_CC
Cuts at: 0 203 1410
Size: 203 1207
NcoI C'CATG_G
Cuts at: 0 242 1410
Size: 242 1168
NgoMIV G'CCGG_C
Cuts at: 0 28 408 1410
Size: 28 380 1002
Fragments arranged by size:
1002 380 28
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 661 693 1021 1053 1410
Size: 661 32 328 32 357
Fragments arranged by size:
661 357 328 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 522 1410
Size: 522 888
PspXI vC'TCGA_Gb
Cuts at: 0 1081 1410
Size: 1081 329
PstI C_TGCA'G
Cuts at: 0 305 719 842 1410
Size: 305 414 123 568
Fragments arranged by size:
568 414 305 123
PvuII CAG'CTG
Cuts at: 0 426 1410
Size: 426 984
SacI G_AGCT'C
Cuts at: 0 157 311 1145 1410
Size: 157 154 834 265
Fragments arranged by size:
834 265 157 154
SapI GCTCTTCn'nnn_
Cuts at: 0 601 1410
Size: 601 809
SbfI CC_TGCA'GG
Cuts at: 0 719 842 1410
Size: 719 123 568
Fragments arranged by size:
719 568 123
SfcI C'TryA_G
Cuts at: 0 301 715 838 1410
Size: 301 414 123 572
Fragments arranged by size:
572 414 301 123
SfoI GGC'GCC
Cuts at: 0 204 1410
Size: 204 1206
SmaI CCC'GGG
Cuts at: 0 87 397 1410
Size: 87 310 1013
Fragments arranged by size:
1013 310 87
SmlI C'TyrA_G
Cuts at: 0 535 682 1081 1410
Size: 535 147 399 329
Fragments arranged by size:
535 399 329 147
StyI C'CwwG_G
Cuts at: 0 242 1410
Size: 242 1168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 52 1410
Size: 52 1358
Tth111I GACn'n_nGTC
Cuts at: 0 1356 1410
Size: 1356 54
XhoI C'TCGA_G
Cuts at: 0 1081 1410
Size: 1081 329
XmaI C'CCGG_G
Cuts at: 0 85 395 1410
Size: 85 310 1015
Fragments arranged by size:
1015 310 85
Enzymes that do cut:
AarI AcuI AfeI AlfI AloI AlwNI AvaI BaeI
BanI BanII BbeI BbsI BbvCI BcgI BclI BglI
BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstYI
Bsu36I BtgI BtgZI BtsI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MmeI
MscI MspA1I NaeI NarI NcoI NgoMIV PpiI PpuMI
PspXI PstI PvuII SacI SapI SbfI SfcI SfoI
SmaI SmlI StyI TaqII Tth111I XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BamHI BciVI BfrBI BglII BmrI BmtI BplI BsaAI
BsaBI BsiWI BsmI BspEI BspHI BsrDI BsrGI BstBI
BstEII BstXI BstZ17I ClaI CspCI DraI DraIII DrdI
EagI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MslI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PshAI PsiI PspOMI PsrI PvuI RsrII
SacII SalI SanDI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI XbaI XcmI XmnI ZraI