Restriction map for
pF1KB7986
(Linear) (Six-base) MAPSORT of: KIBB7986.seq Check: 1382 from: 1 to: 975
KIBB7986.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 0 870 975
Size: 870 105
Acc65I G'GTAC_C
Cuts at: 0 764 975
Size: 764 211
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 913 975
Size: 683 230 62
Fragments arranged by size:
683 230 62
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 770 802 975
Size: 770 32 173
Fragments arranged by size:
770 173 32
AlwNI CAG_nnn'CTG
Cuts at: 0 534 975
Size: 534 441
ApaLI G'TGCA_C
Cuts at: 0 968 975
Size: 968 7
AseI AT'TA_AT
Cuts at: 0 47 80 975
Size: 47 33 895
Fragments arranged by size:
895 47 33
AvaI C'yCGr_G
Cuts at: 0 63 975
Size: 63 912
BanI G'GyrC_C
Cuts at: 0 764 975
Size: 764 211
BanII G_rGCy'C
Cuts at: 0 71 975
Size: 71 904
BbsI GAAGACnn'nnnn_
Cuts at: 0 873 975
Size: 873 102
BglII A'GATC_T
Cuts at: 0 888 975
Size: 888 87
BlpI GC'TnA_GC
Cuts at: 0 309 975
Size: 309 666
Bme1580I G_kGCm'C
Cuts at: 0 972 975
Size: 972 3
BmgBI CAC'GTC
Cuts at: 0 227 975
Size: 227 748
BmrI ACTGGGnnnn_n'
Cuts at: 0 586 928 975
Size: 586 342 47
Fragments arranged by size:
586 342 47
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 803 975
Size: 524 279 172
Fragments arranged by size:
524 279 172
BsaI GGTCTCn'nnnn_
Cuts at: 0 18 975
Size: 18 957
BsaWI w'CCGG_w
Cuts at: 0 164 326 938 975
Size: 164 162 612 37
Fragments arranged by size:
612 164 162 37
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 289 841 975
Size: 103 186 552 134
Fragments arranged by size:
552 186 134 103
BseYI C'CCAG_C
Cuts at: 0 134 170 975
Size: 134 36 805
Fragments arranged by size:
805 134 36
BsiHKAI G_wGCw'C
Cuts at: 0 972 975
Size: 972 3
Bsp1286I G_dGCh'C
Cuts at: 0 71 972 975
Size: 71 901 3
Fragments arranged by size:
901 71 3
BspEI T'CCGG_A
Cuts at: 0 164 975
Size: 164 811
BspHI T'CATG_A
Cuts at: 0 710 975
Size: 710 265
BsrGI T'GTAC_A
Cuts at: 0 626 975
Size: 626 349
BstXI CCAn_nnnn'nTGG
Cuts at: 0 915 975
Size: 915 60
BstYI r'GATC_y
Cuts at: 0 104 216 888 975
Size: 104 112 672 87
Fragments arranged by size:
672 112 104 87
Bsu36I CC'TnA_GG
Cuts at: 0 701 975
Size: 701 274
BtgI C'CryG_G
Cuts at: 0 109 975
Size: 109 866
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 731 766 975
Size: 731 35 209
Fragments arranged by size:
731 209 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 683 803 913 975
Size: 524 159 120 110 62
Fragments arranged by size:
524 159 120 110 62
EcoO109I rG'GnC_Cy
Cuts at: 0 531 975
Size: 531 444
EcoRV GAT'ATC
Cuts at: 0 270 975
Size: 270 705
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 255 975
Size: 255 720
FspI TGC'GCA
Cuts at: 0 19 975
Size: 19 956
FspAI rTGC'GCAy
Cuts at: 0 19 975
Size: 19 956
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 427 459 975
Size: 427 32 516
Fragments arranged by size:
516 427 32
KpnI G_GTAC'C
Cuts at: 0 768 975
Size: 768 207
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 710 975
Size: 77 633 265
Fragments arranged by size:
633 265 77
MslI CAynn'nnrTG
Cuts at: 0 13 592 975
Size: 13 579 383
Fragments arranged by size:
579 383 13
MspA1I CmG'CkG
Cuts at: 0 313 975
Size: 313 662
NcoI C'CATG_G
Cuts at: 0 109 975
Size: 109 866
NspI r_CATG'y
Cuts at: 0 121 510 975
Size: 121 389 465
Fragments arranged by size:
465 389 121
PasI CC'CwG_GG
Cuts at: 0 847 975
Size: 847 128
PflMI CCAn_nnn'nTGG
Cuts at: 0 227 975
Size: 227 748
PshAI GACnn'nnGTC
Cuts at: 0 934 975
Size: 934 41
PvuII CAG'CTG
Cuts at: 0 313 975
Size: 313 662
SfcI C'TryA_G
Cuts at: 0 871 975
Size: 871 104
SmaI CCC'GGG
Cuts at: 0 65 975
Size: 65 910
SphI G_CATG'C
Cuts at: 0 121 975
Size: 121 854
StyI C'CwwG_G
Cuts at: 0 109 975
Size: 109 866
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 660 975
Size: 660 315
TatI w'GTAC_w
Cuts at: 0 626 975
Size: 626 349
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 402 434 975
Size: 402 32 541
Fragments arranged by size:
541 402 32
XmaI C'CCGG_G
Cuts at: 0 63 975
Size: 63 912
Enzymes that do cut:
AccI Acc65I AcuI AloI AlwNI ApaLI AseI AvaI
BanI BanII BbsI BglII BlpI Bme1580I BmgBI BmrI
BpmI BsaI BsaWI BseRI BseYI BsiHKAI Bsp1286I BspEI
BspHI BsrGI BstXI BstYI Bsu36I BtgI CspCI Eco57MI
EcoO109I EcoRV FalI FspI FspAI Hin4I KpnI MmeI
MslI MspA1I NcoI NspI PasI PflMI PshAI PvuII
SfcI SmaI SphI StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI ApaI ApoI AscI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI EcoNI EcoRI FseI HaeII HincII HindIII
HpaI KasI MfeI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PfoI
PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmnI ZraI