Restriction map for
pF1KB7984
(Linear) (Six-base) MAPSORT of: KIBB7984.seq Check: 3671 from: 1 to: 972
KIBB7984.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AflIII A'CryG_T
Cuts at: 0 353 527 845 847 972
Size: 353 174 318 2 125
Fragments arranged by size:
353 318 174 125 2
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 913 945 972
Size: 913 32 27
Fragments arranged by size:
913 32 27
AlwNI CAG_nnn'CTG
Cuts at: 0 318 972
Size: 318 654
ApaLI G'TGCA_C
Cuts at: 0 166 972
Size: 166 806
ApoI r'AATT_y
Cuts at: 0 780 972
Size: 780 192
AvaI C'yCGr_G
Cuts at: 0 401 723 855 972
Size: 401 322 132 117
Fragments arranged by size:
401 322 132 117
BanI G'GyrC_C
Cuts at: 0 244 699 789 972
Size: 244 455 90 183
Fragments arranged by size:
455 244 183 90
BanII G_rGCy'C
Cuts at: 0 509 730 972
Size: 509 221 242
Fragments arranged by size:
509 242 221
BbeI G_GCGC'C
Cuts at: 0 248 972
Size: 248 724
BbvCI CC'TCA_GC
Cuts at: 0 705 972
Size: 705 267
Bme1580I G_kGCm'C
Cuts at: 0 170 704 972
Size: 170 534 268
Fragments arranged by size:
534 268 170
BmgBI CAC'GTC
Cuts at: 0 240 972
Size: 240 732
BmrI ACTGGGnnnn_n'
Cuts at: 0 564 972
Size: 564 408
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 181 295 340 714 879 972
Size: 171 10 114 45 374 165 93
Fragments arranged by size:
374 171 165 114 93 45 10
Bpu10I CC'TnA_GC
Cuts at: 0 705 972
Size: 705 267
BsaAI yAC'GTr
Cuts at: 0 848 972
Size: 848 124
BsaHI Gr'CG_yC
Cuts at: 0 245 719 796 972
Size: 245 474 77 176
Fragments arranged by size:
474 245 176 77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 160 190 913 943 972
Size: 160 30 723 30 29
Fragments arranged by size:
723 160 30 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 57 135 815 972
Size: 57 78 680 157
Fragments arranged by size:
680 157 78 57
BseYI C'CCAG_C
Cuts at: 0 89 972
Size: 89 883
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 972
Size: 937 35
BsiEI CG_ry'CG
Cuts at: 0 62 136 972
Size: 62 74 836
Fragments arranged by size:
836 74 62
BsiHKAI G_wGCw'C
Cuts at: 0 35 170 972
Size: 35 135 802
Fragments arranged by size:
802 135 35
BsmBI CGTCTCn'nnnn_
Cuts at: 0 69 972
Size: 69 903
Bsp1286I G_dGCh'C
Cuts at: 0 35 170 509 704 730 972
Size: 35 135 339 195 26 242
Fragments arranged by size:
339 242 195 135 35 26
BsrFI r'CCGG_y
Cuts at: 0 962 972
Size: 962 10
BtgI C'CryG_G
Cuts at: 0 101 785 972
Size: 101 684 187
Fragments arranged by size:
684 187 101
BtsI GCAGTG_nn'
Cuts at: 0 716 962 972
Size: 716 246 10
Fragments arranged by size:
716 246 10
DrdI GACnn_nn'nnGTC
Cuts at: 0 54 972
Size: 54 918
EaeI y'GGCC_r
Cuts at: 0 59 863 972
Size: 59 804 109
Fragments arranged by size:
804 109 59
EagI C'GGCC_G
Cuts at: 0 59 972
Size: 59 913
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 567 972
Size: 567 405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 181 295 340 714 879 972
Size: 171 10 114 45 374 165 93
Fragments arranged by size:
374 171 165 114 93 45 10
EcoO109I rG'GnC_Cy
Cuts at: 0 252 567 972
Size: 252 315 405
Fragments arranged by size:
405 315 252
EcoRI G'AATT_C
Cuts at: 0 780 972
Size: 780 192
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 37 501 972
Size: 37 464 471
Fragments arranged by size:
471 464 37
HaeII r_GCGC'y
Cuts at: 0 248 972
Size: 248 724
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 241 273 972
Size: 241 32 699
Fragments arranged by size:
699 241 32
HincII GTy'rAC
Cuts at: 0 627 972
Size: 627 345
KasI G'GCGC_C
Cuts at: 0 244 972
Size: 244 728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 956 972
Size: 203 753 16
Fragments arranged by size:
753 203 16
MscI TGG'CCA
Cuts at: 0 865 972
Size: 865 107
MslI CAynn'nnrTG
Cuts at: 0 682 972
Size: 682 290
MspA1I CmG'CkG
Cuts at: 0 43 285 787 972
Size: 43 242 502 185
Fragments arranged by size:
502 242 185 43
NarI GG'CG_CC
Cuts at: 0 245 972
Size: 245 727
NcoI C'CATG_G
Cuts at: 0 101 972
Size: 101 871
NspI r_CATG'y
Cuts at: 0 357 531 651 687 972
Size: 357 174 120 36 285
Fragments arranged by size:
357 285 174 120 36
PciI A'CATG_T
Cuts at: 0 353 527 972
Size: 353 174 445
Fragments arranged by size:
445 353 174
PflMI CCAn_nnn'nTGG
Cuts at: 0 318 972
Size: 318 654
PfoI T'CCnGG_A
Cuts at: 0 311 972
Size: 311 661
PmlI CAC'GTG
Cuts at: 0 848 972
Size: 848 124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 913 945 972
Size: 913 32 27
Fragments arranged by size:
913 32 27
PpuMI rG'GwC_Cy
Cuts at: 0 252 972
Size: 252 720
PshAI GACnn'nnGTC
Cuts at: 0 718 972
Size: 718 254
PspXI vC'TCGA_Gb
Cuts at: 0 855 972
Size: 855 117
SacII CC_GC'GG
Cuts at: 0 788 972
Size: 788 184
SfoI GGC'GCC
Cuts at: 0 246 972
Size: 246 726
SmaI CCC'GGG
Cuts at: 0 725 972
Size: 725 247
SmlI C'TyrA_G
Cuts at: 0 855 972
Size: 855 117
StyI C'CwwG_G
Cuts at: 0 101 563 972
Size: 101 462 409
Fragments arranged by size:
462 409 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 675 972
Size: 675 297
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 160 192 972
Size: 160 32 780
Fragments arranged by size:
780 160 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 156 972
Size: 156 816
XhoI C'TCGA_G
Cuts at: 0 855 972
Size: 855 117
XmaI C'CCGG_G
Cuts at: 0 723 972
Size: 723 249
XmnI GAAnn'nnTTC
Cuts at: 0 484 972
Size: 484 488
Enzymes that do cut:
AflIII AloI AlwNI ApaLI ApoI AvaI BanI BanII
BbeI BbvCI Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BsrFI BtgI BtsI DrdI EaeI EagI EciI
Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NarI NcoI NspI PciI
PflMI PfoI PmlI PpiI PpuMI PshAI PspXI SacII
SfoI SmaI SmlI StyI TaqII TstI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmtI BplI BpuEI BsaI BsaBI
BsaWI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EarI
EcoICRI EcoNI EcoRV FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PmeI PsiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI Tth111I
XbaI ZraI