Restriction map for
pF1KB7973
(Linear) (Six-base) MAPSORT of: KIBB7973.seq Check: 5890 from: 1 to: 642
KIBB7973.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 540 642
Size: 187 353 102
Fragments arranged by size:
353 187 102
AflIII A'CryG_T
Cuts at: 0 467 642
Size: 467 175
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 478 510 642
Size: 478 32 132
Fragments arranged by size:
478 132 32
AlwNI CAG_nnn'CTG
Cuts at: 0 540 591 642
Size: 540 51 51
Fragments arranged by size:
540 51 51
AvaI C'yCGr_G
Cuts at: 0 22 642
Size: 22 620
BanI G'GyrC_C
Cuts at: 0 13 642
Size: 13 629
BbeI G_GCGC'C
Cuts at: 0 17 642
Size: 17 625
BbsI GAAGACnn'nnnn_
Cuts at: 0 546 642
Size: 546 96
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 252 286 642
Size: 252 34 356
Fragments arranged by size:
356 252 34
BlpI GC'TnA_GC
Cuts at: 0 158 259 642
Size: 158 101 383
Fragments arranged by size:
383 158 101
BsaI GGTCTCn'nnnn_
Cuts at: 0 450 642
Size: 450 192
BsaHI Gr'CG_yC
Cuts at: 0 14 176 611 642
Size: 14 162 435 31
Fragments arranged by size:
435 162 31 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 325 355 642
Size: 325 30 287
Fragments arranged by size:
325 287 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 183 346 349 583 642
Size: 183 163 3 234 59
Fragments arranged by size:
234 183 163 59 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 642
Size: 601 41
BsiHKAI G_wGCw'C
Cuts at: 0 575 642
Size: 575 67
Bsp1286I G_dGCh'C
Cuts at: 0 575 642
Size: 575 67
BspHI T'CATG_A
Cuts at: 0 377 642
Size: 377 265
BsrFI r'CCGG_y
Cuts at: 0 131 642
Size: 131 511
BssHII G'CGCG_C
Cuts at: 0 141 642
Size: 141 501
BstXI CCAn_nnnn'nTGG
Cuts at: 0 522 642
Size: 522 120
DrdI GACnn_nn'nnGTC
Cuts at: 0 34 642
Size: 34 608
EaeI y'GGCC_r
Cuts at: 0 524 642
Size: 524 118
EarI CTCTTCn'nnn_
Cuts at: 0 160 642
Size: 160 482
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 540 642
Size: 187 353 102
Fragments arranged by size:
353 187 102
EcoO109I rG'GnC_Cy
Cuts at: 0 27 642
Size: 27 615
HaeII r_GCGC'y
Cuts at: 0 17 642
Size: 17 625
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 325 357 430 462 642
Size: 325 32 73 32 180
Fragments arranged by size:
325 180 73 32 32
KasI G'GCGC_C
Cuts at: 0 13 642
Size: 13 629
MfeI C'AATT_G
Cuts at: 0 622 642
Size: 622 20
MscI TGG'CCA
Cuts at: 0 526 642
Size: 526 116
MslI CAynn'nnrTG
Cuts at: 0 382 520 642
Size: 382 138 122
Fragments arranged by size:
382 138 122
MspA1I CmG'CkG
Cuts at: 0 560 642
Size: 560 82
NarI GG'CG_CC
Cuts at: 0 14 642
Size: 14 628
PfoI T'CCnGG_A
Cuts at: 0 533 642
Size: 533 109
PpuMI rG'GwC_Cy
Cuts at: 0 27 642
Size: 27 615
PshAI GACnn'nnGTC
Cuts at: 0 211 642
Size: 211 431
PvuII CAG'CTG
Cuts at: 0 560 642
Size: 560 82
SexAI A'CCwGG_T
Cuts at: 0 440 452 642
Size: 440 12 190
Fragments arranged by size:
440 190 12
SfcI C'TryA_G
Cuts at: 0 54 642
Size: 54 588
SfoI GGC'GCC
Cuts at: 0 15 642
Size: 15 627
StuI AGG'CCT
Cuts at: 0 109 642
Size: 109 533
StyI C'CwwG_G
Cuts at: 0 31 642
Size: 31 611
Tth111I GACn'n_nGTC
Cuts at: 0 442 454 642
Size: 442 12 188
Fragments arranged by size:
442 188 12
Enzymes that do cut:
AcuI AflIII AloI AlwNI AvaI BanI BbeI BbsI
BcgI BlpI BsaI BsaHI BsaXI BseRI BsgI BsiHKAI
Bsp1286I BspHI BsrFI BssHII BstXI DrdI EaeI EarI
Eco57MI EcoO109I HaeII Hin4I KasI MfeI MscI MslI
MspA1I NarI PfoI PpuMI PshAI PvuII SexAI SfcI
SfoI StuI StyI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbvCI BciVI BclI
BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BseYI BsiEI
BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrGI
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MluI MmeI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TatI TstI XbaI XcmI XhoI XmaI XmnI ZraI