(Linear) (Six-base) MAPSORT of: KIBB7961.seq  Check: 9927  from: 1  to: 795



KIBB7961.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     428     578     795 
   Size:        428     150     217

  Fragments arranged by size: 

                 428     217     150
 
AleI CACnn'nnGTG

Cuts at:      0     774     795 
   Size:        774      21
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      76      93     795 
   Size:         76      17     702

  Fragments arranged by size: 

                 702      76      17
 
ApoI r'AATT_y

Cuts at:      0     529     602     719     754     795 
   Size:        529      73     117      35      41

  Fragments arranged by size: 

                 529     117      73      41      35
 
BanII G_rGCy'C

Cuts at:      0     696     795 
   Size:        696      99
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     791     795 
   Size:        791       4
 
BfrBI ATG'CAT

Cuts at:      0      70     795 
   Size:         70     725
 
BglI GCCn_nnn'nGGC

Cuts at:      0     341     795 
   Size:        341     454
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     349     795 
   Size:        202     147     446

  Fragments arranged by size: 

                 446     202     147
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     533     795 
   Size:        533     262
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     328     795 
   Size:        328     467
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     318     795 
   Size:        318     477
 
BsmI GAATG_Cn'

Cuts at:      0     572     795 
   Size:        572     223
 
Bsp1286I G_dGCh'C

Cuts at:      0     696     795 
   Size:        696      99
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     551     795 
   Size:        551     244
 
BsrBI CCG'CTC

Cuts at:      0     729     795 
   Size:        729      66
 
BsrDI GCAATG_nn'

Cuts at:      0     120     795 
   Size:        120     675
 
BsrGI T'GTAC_A

Cuts at:      0     749     795 
   Size:        749      46
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     264     299     795 
   Size:        264      35     496

  Fragments arranged by size: 

                 496     264      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     113     795 
   Size:        113     682
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     349     428     578     795 
   Size:        202     147      79     150     217

  Fragments arranged by size: 

                 217     202     150     147      79
 
HaeII r_GCGC'y

Cuts at:      0     201     795 
   Size:        201     594
 
HincII GTy'rAC

Cuts at:      0     735     795 
   Size:        735      60
 
MfeI C'AATT_G

Cuts at:      0     252     795 
   Size:        252     543
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      14     224     470     795 
   Size:         14     210     246     325

  Fragments arranged by size: 

                 325     246     210      14
 
MslI CAynn'nnrTG

Cuts at:      0     774     795 
   Size:        774      21
 
MspA1I CmG'CkG

Cuts at:      0     294     331     795 
   Size:        294      37     464

  Fragments arranged by size: 

                 464     294      37
 
NdeI CA'TA_TG

Cuts at:      0     675     795 
   Size:        675     120
 
NsiI A_TGCA'T

Cuts at:      0      72     795 
   Size:         72     723
 
PshAI GACnn'nnGTC

Cuts at:      0     172     795 
   Size:        172     623
 
PstI C_TGCA'G

Cuts at:      0     293     795 
   Size:        293     502
 
PvuII CAG'CTG

Cuts at:      0     294     331     795 
   Size:        294      37     464

  Fragments arranged by size: 

                 464     294      37
 
ScaI AGT'ACT

Cuts at:      0     124     795 
   Size:        124     671
 
SfcI C'TryA_G

Cuts at:      0     289     795 
   Size:        289     506
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     752     795 
   Size:        752      43
 
TatI w'GTAC_w

Cuts at:      0     122     236     353     749     795 
   Size:        122     114     117     396      46

  Fragments arranged by size: 

                 396     122     117     114      46
 

 Enzymes that do cut:

     AcuI     AleI     AlfI     ApoI    BanII     BbsI    BfrBI     BglI
     BpmI     BsaI    BseRI     BsgI     BsmI Bsp1286I    BspMI    BsrBI
    BsrDI    BsrGI    CspCI     EarI  Eco57MI    HaeII   HincII     MfeI
     MmeI     MslI   MspA1I     NdeI     NsiI    PshAI     PstI    PvuII
     ScaI     SfcI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I  HindIII     HpaI
     KasI     KpnI     MluI     MscI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI