Restriction map for
pF1KB7948
(Linear) (Six-base) MAPSORT of: KIBB7948.seq Check: 3293 from: 1 to: 1125
KIBB7948.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 540 1125
Size: 540 585
AccI GT'mk_AC
Cuts at: 0 962 1125
Size: 962 163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 1125
Size: 61 1064
AflIII A'CryG_T
Cuts at: 0 801 1125
Size: 801 324
AlwNI CAG_nnn'CTG
Cuts at: 0 187 1125
Size: 187 938
ApoI r'AATT_y
Cuts at: 0 160 215 1125
Size: 160 55 910
Fragments arranged by size:
910 160 55
AvaI C'yCGr_G
Cuts at: 0 377 1125
Size: 377 748
BbsI GAAGACnn'nnnn_
Cuts at: 0 14 1125
Size: 14 1111
BlpI GC'TnA_GC
Cuts at: 0 1040 1125
Size: 1040 85
BmrI ACTGGGnnnn_n'
Cuts at: 0 252 1125
Size: 252 873
BsaAI yAC'GTr
Cuts at: 0 36 1125
Size: 36 1089
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1052 1070 1125
Size: 1052 18 55
Fragments arranged by size:
1052 55 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 776 1125
Size: 518 258 349
Fragments arranged by size:
518 349 258
BsiHKAI G_wGCw'C
Cuts at: 0 1041 1125
Size: 1041 84
Bsp1286I G_dGCh'C
Cuts at: 0 1041 1125
Size: 1041 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 540 1125
Size: 540 585
BsrDI GCAATG_nn'
Cuts at: 0 144 1125
Size: 144 981
BstAPI GCAn_nnn'nTGC
Cuts at: 0 187 196 457 763 1125
Size: 187 9 261 306 362
Fragments arranged by size:
362 306 261 187 9
BstEII G'GTnAC_C
Cuts at: 0 40 1125
Size: 40 1085
BstXI CCAn_nnnn'nTGG
Cuts at: 0 898 1125
Size: 898 227
BstZ17I GTA'TAC
Cuts at: 0 963 1125
Size: 963 162
BtgI C'CryG_G
Cuts at: 0 973 1125
Size: 973 152
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 1125
Size: 61 1064
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1049 1125
Size: 1049 76
EcoRV GAT'ATC
Cuts at: 0 336 1125
Size: 336 789
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 325 357 1125
Size: 325 32 768
Fragments arranged by size:
768 325 32
HincII GTy'rAC
Cuts at: 0 647 864 1125
Size: 647 217 261
Fragments arranged by size:
647 261 217
HpaI GTT'AAC
Cuts at: 0 647 1125
Size: 647 478
MfeI C'AATT_G
Cuts at: 0 510 1125
Size: 510 615
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 879 1125
Size: 879 246
MslI CAynn'nnrTG
Cuts at: 0 176 290 752 1125
Size: 176 114 462 373
Fragments arranged by size:
462 373 176 114
MspA1I CmG'CkG
Cuts at: 0 184 1125
Size: 184 941
NcoI C'CATG_G
Cuts at: 0 973 1125
Size: 973 152
NdeI CA'TA_TG
Cuts at: 0 470 1125
Size: 470 655
NspI r_CATG'y
Cuts at: 0 805 1125
Size: 805 320
PciI A'CATG_T
Cuts at: 0 801 1125
Size: 801 324
PshAI GACnn'nnGTC
Cuts at: 0 64 869 1125
Size: 64 805 256
Fragments arranged by size:
805 256 64
PsiI TTA'TAA
Cuts at: 0 437 1125
Size: 437 688
PvuII CAG'CTG
Cuts at: 0 184 1125
Size: 184 941
SfcI C'TryA_G
Cuts at: 0 867 1125
Size: 867 258
SmaI CCC'GGG
Cuts at: 0 379 1125
Size: 379 746
SnaBI TAC'GTA
Cuts at: 0 36 1125
Size: 36 1089
StyI C'CwwG_G
Cuts at: 0 973 1050 1125
Size: 973 77 75
Fragments arranged by size:
973 77 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 478 926 1015 1125
Size: 478 448 89 110
Fragments arranged by size:
478 448 110 89
TatI w'GTAC_w
Cuts at: 0 544 613 620 1125
Size: 544 69 7 505
Fragments arranged by size:
544 505 69 7
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 298 1096 1125
Size: 298 798 29
Fragments arranged by size:
798 298 29
XmaI C'CCGG_G
Cuts at: 0 377 1125
Size: 377 748
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApoI AvaI BbsI
BlpI BmrI BsaAI BseRI BsgI BsiHKAI Bsp1286I BspMI
BsrDI BstAPI BstEII BstXI BstZ17I BtgI Eco57MI EcoNI
EcoRV Hin4I HincII HpaI MfeI MmeI MslI MspA1I
NcoI NdeI NspI PciI PshAI PsiI PvuII SfcI
SmaI SnaBI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII Bme1580I BmgBI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstYI Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI EcoO109I EcoRI FalI FseI FspI
FspAI HaeII HindIII KasI KpnI MluI MscI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XhoI XmnI ZraI