Restriction map for
pF1KB7925
(Linear) (Six-base) MAPSORT of: KIBB7925.seq Check: 9273 from: 1 to: 576
KIBB7925.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 207 469 576
Size: 207 262 107
Fragments arranged by size:
262 207 107
ApaI G_GGCC'C
Cuts at: 0 240 576
Size: 240 336
ApoI r'AATT_y
Cuts at: 0 36 576
Size: 36 540
AvaI C'yCGr_G
Cuts at: 0 91 115 576
Size: 91 24 461
Fragments arranged by size:
461 91 24
BamHI G'GATC_C
Cuts at: 0 225 576
Size: 225 351
BanII G_rGCy'C
Cuts at: 0 60 130 141 240 576
Size: 60 70 11 99 336
Fragments arranged by size:
336 99 70 60 11
BbvCI CC'TCA_GC
Cuts at: 0 386 576
Size: 386 190
BfrBI ATG'CAT
Cuts at: 0 560 576
Size: 560 16
BglI GCCn_nnn'nGGC
Cuts at: 0 388 576
Size: 388 188
Bme1580I G_kGCm'C
Cuts at: 0 240 576
Size: 240 336
BmrI ACTGGGnnnn_n'
Cuts at: 0 74 576
Size: 74 502
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 144 576
Size: 144 432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 259 397 494 576
Size: 170 89 138 97 82
Fragments arranged by size:
170 138 97 89 82
Bpu10I CC'TnA_GC
Cuts at: 0 386 576
Size: 386 190
BsaI GGTCTCn'nnnn_
Cuts at: 0 88 576
Size: 88 488
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 421 451 576
Size: 421 30 125
Fragments arranged by size:
421 125 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 576
Size: 133 443
BseYI C'CCAG_C
Cuts at: 0 250 496 576
Size: 250 246 80
Fragments arranged by size:
250 246 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 576
Size: 427 149
BsiHKAI G_wGCw'C
Cuts at: 0 130 576
Size: 130 446
Bsp1286I G_dGCh'C
Cuts at: 0 60 130 141 240 576
Size: 60 70 11 99 336
Fragments arranged by size:
336 99 70 60 11
BstYI r'GATC_y
Cuts at: 0 225 576
Size: 225 351
EaeI y'GGCC_r
Cuts at: 0 150 576
Size: 150 426
EarI CTCTTCn'nnn_
Cuts at: 0 305 576
Size: 305 271
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 499 576
Size: 499 77
EcoICRI GAG'CTC
Cuts at: 0 128 576
Size: 128 448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 259 397 494 576
Size: 170 89 138 97 82
Fragments arranged by size:
170 138 97 89 82
EcoO109I rG'GnC_Cy
Cuts at: 0 156 236 237 318 420 576
Size: 156 80 1 81 102 156
Fragments arranged by size:
156 156 102 81 80 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 421 453 576
Size: 421 32 123
Fragments arranged by size:
421 123 32
MscI TGG'CCA
Cuts at: 0 152 576
Size: 152 424
MspA1I CmG'CkG
Cuts at: 0 369 385 576
Size: 369 16 191
Fragments arranged by size:
369 191 16
NdeI CA'TA_TG
Cuts at: 0 256 576
Size: 256 320
NsiI A_TGCA'T
Cuts at: 0 562 576
Size: 562 14
NspI r_CATG'y
Cuts at: 0 84 576
Size: 84 492
PflMI CCAn_nnn'nTGG
Cuts at: 0 342 576
Size: 342 234
PpuMI rG'GwC_Cy
Cuts at: 0 318 576
Size: 318 258
PspOMI G'GGCC_C
Cuts at: 0 236 576
Size: 236 340
PstI C_TGCA'G
Cuts at: 0 89 311 576
Size: 89 222 265
Fragments arranged by size:
265 222 89
SacI G_AGCT'C
Cuts at: 0 130 576
Size: 130 446
SfcI C'TryA_G
Cuts at: 0 85 307 576
Size: 85 222 269
Fragments arranged by size:
269 222 85
SmlI C'TyrA_G
Cuts at: 0 570 576
Size: 570 6
StyI C'CwwG_G
Cuts at: 0 132 194 576
Size: 132 62 382
Fragments arranged by size:
382 132 62
TatI w'GTAC_w
Cuts at: 0 181 576
Size: 181 395
Enzymes that do cut:
AlwNI ApaI ApoI AvaI BamHI BanII BbvCI BfrBI
BglI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BstYI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I Hin4I MscI MspA1I NdeI
NsiI NspI PflMI PpuMI PspOMI PstI SacI SfcI
SmlI StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BanI BbeI BbsI BcgI
BciVI BclI BglII BlpI BmgBI BmtI BpuEI BsaAI
BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MslI
NaeI NarI NcoI NgoMIV NheI NotI NruI PacI
PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI