(Linear) (Six-base) MAPSORT of: KIBB7913.seq  Check: 7564  from: 1  to: 483



KIBB7913.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     419     483 
   Size:        419      64
 
AflIII A'CryG_T

Cuts at:      0     374     483 
   Size:        374     109
 
BamHI G'GATC_C

Cuts at:      0     210     380     483 
   Size:        210     170     103

  Fragments arranged by size: 

                 210     170     103
 
BbvCI CC'TCA_GC

Cuts at:      0      18     483 
   Size:         18     465
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     483 
   Size:        352     131
 
Bpu10I CC'TnA_GC

Cuts at:      0      18     483 
   Size:         18     465
 
BsaWI w'CCGG_w

Cuts at:      0      57     464     483 
   Size:         57     407      19

  Fragments arranged by size: 

                 407      57      19
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     256     483 
   Size:        256     227
 
BspEI T'CCGG_A

Cuts at:      0     464     483 
   Size:        464      19
 
BstYI r'GATC_y

Cuts at:      0     210     380     483 
   Size:        210     170     103

  Fragments arranged by size: 

                 210     170     103
 
BtgI C'CryG_G

Cuts at:      0      89     483 
   Size:         89     394
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     298     483 
   Size:        298     185
 
BtsI GCAGTG_nn'

Cuts at:      0     113     483 
   Size:        113     370
 
EaeI y'GGCC_r

Cuts at:      0      92     483 
   Size:         92     391
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     419     483 
   Size:        352      67      64

  Fragments arranged by size: 

                 352      67      64
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      80     134     483 
   Size:         80      54     349

  Fragments arranged by size: 

                 349      80      54
 
HaeII r_GCGC'y

Cuts at:      0     366     483 
   Size:        366     117
 
HindIII A'AGCT_T

Cuts at:      0     422     483 
   Size:        422      61
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     432     483 
   Size:        206     226      51

  Fragments arranged by size: 

                 226     206      51
 
MscI TGG'CCA

Cuts at:      0      94     483 
   Size:         94     389
 
MspA1I CmG'CkG

Cuts at:      0     351     483 
   Size:        351     132
 
NcoI C'CATG_G

Cuts at:      0      89     483 
   Size:         89     394
 
NspI r_CATG'y

Cuts at:      0     378     483 
   Size:        378     105
 
PciI A'CATG_T

Cuts at:      0     374     483 
   Size:        374     109
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     391     483 
   Size:        391      92
 
PstI C_TGCA'G

Cuts at:      0     108     257     483 
   Size:        108     149     226

  Fragments arranged by size: 

                 226     149     108
 
PvuII CAG'CTG

Cuts at:      0     351     483 
   Size:        351     132
 
ScaI AGT'ACT

Cuts at:      0     102     483 
   Size:        102     381
 
SfcI C'TryA_G

Cuts at:      0     104     253     483 
   Size:        104     149     230

  Fragments arranged by size: 

                 230     149     104
 
StyI C'CwwG_G

Cuts at:      0      89     483 
   Size:         89     394
 
TatI w'GTAC_w

Cuts at:      0     100     483 
   Size:        100     383
 

 Enzymes that do cut:

     AcuI   AflIII    BamHI    BbvCI     BpmI   Bpu10I    BsaWI    BseRI
    BspEI    BstYI     BtgI    BtgZI     BtsI     EaeI  Eco57MI    EcoNI
    HaeII  HindIII     MmeI     MscI   MspA1I     NcoI     NspI     PciI
    PflMI     PstI    PvuII     ScaI     SfcI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI