(Linear) (Six-base) MAPSORT of: KIBB7911.seq  Check: 1310  from: 1  to: 441



KIBB7911.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AccI GT'mk_AC

Cuts at:      0     434     441 
   Size:        434       7
 
AvaI C'yCGr_G

Cuts at:      0      13     441 
   Size:         13     428
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     120     190     392     441 
   Size:        120      70     202      49

  Fragments arranged by size: 

                 202     120      70      49
 
BclI T'GATC_A

Cuts at:      0      41     441 
   Size:         41     400
 
BsiHKAI G_wGCw'C

Cuts at:      0     209     441 
   Size:        209     232
 
BsmI GAATG_Cn'

Cuts at:      0     198     441 
   Size:        198     243
 
Bsp1286I G_dGCh'C

Cuts at:      0     209     441 
   Size:        209     232
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      35     441 
   Size:         35     406
 
BsrDI GCAATG_nn'

Cuts at:      0     173     441 
   Size:        173     268
 
BtgI C'CryG_G

Cuts at:      0     215     441 
   Size:        215     226
 
EarI CTCTTCn'nnn_

Cuts at:      0     215     344     416     441 
   Size:        215     129      72      25

  Fragments arranged by size: 

                 215     129      72      25
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     441 
   Size:         19     422
 
EcoO109I rG'GnC_Cy

Cuts at:      0      18     441 
   Size:         18     423
 
HaeII r_GCGC'y

Cuts at:      0      67     107     441 
   Size:         67      40     334

  Fragments arranged by size: 

                 334      67      40
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     193     225     441 
   Size:        193      32     216

  Fragments arranged by size: 

                 216     193      32
 
HincII GTy'rAC

Cuts at:      0      81     441 
   Size:         81     360
 
MspA1I CmG'CkG

Cuts at:      0     237     441 
   Size:        237     204
 
NcoI C'CATG_G

Cuts at:      0     215     441 
   Size:        215     226
 
PfoI T'CCnGG_A

Cuts at:      0      68     441 
   Size:         68     373
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     214     246     441 
   Size:        214      32     195

  Fragments arranged by size: 

                 214     195      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      18     441 
   Size:         18     423
 
SapI GCTCTTCn'nnn_

Cuts at:      0     344     441 
   Size:        344      97
 
StuI AGG'CCT

Cuts at:      0     304     441 
   Size:        304     137
 
StyI C'CwwG_G

Cuts at:      0      89     215     441 
   Size:         89     126     226

  Fragments arranged by size: 

                 226     126      89
 
Tth111I GACn'n_nGTC

Cuts at:      0      76     441 
   Size:         76     365
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     222     441 
   Size:        222     219
 

 Enzymes that do cut:

     AccI     AvaI     BbsI     BclI  BsiHKAI     BsmI Bsp1286I    BspMI
    BsrDI     BtgI     EarI     EciI EcoO109I    HaeII    Hin4I   HincII
   MspA1I     NcoI     PfoI     PpiI    PpuMI     SapI     StuI     StyI
  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI  EcoICRI  Eco57MI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TatI     TstI     XbaI     XhoI     XmaI
     XmnI     ZraI