(Linear) (Six-base) MAPSORT of: KIBB7909.seq  Check: 5881  from: 1  to: 402



KIBB7909.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     286     402 
   Size:        247      39     116

  Fragments arranged by size: 

                 247     116      39
 
AleI CACnn'nnGTG

Cuts at:      0     364     402 
   Size:        364      38
 
BamHI G'GATC_C

Cuts at:      0     115     325     402 
   Size:        115     210      77

  Fragments arranged by size: 

                 210     115      77
 
BanII G_rGCy'C

Cuts at:      0     287     402 
   Size:        287     115
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     235     402 
   Size:        235     167
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     384     402 
   Size:        384      18
 
BglI GCCn_nnn'nGGC

Cuts at:      0      20     342     402 
   Size:         20     322      60

  Fragments arranged by size: 

                 322      60      20
 
BlpI GC'TnA_GC

Cuts at:      0     274     402 
   Size:        274     128
 
Bme1580I G_kGCm'C

Cuts at:      0     338     359     402 
   Size:        338      21      43

  Fragments arranged by size: 

                 338      43      21
 
BseYI C'CCAG_C

Cuts at:      0     128     402 
   Size:        128     274
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     402 
   Size:        163     239
 
BsiEI CG_ry'CG

Cuts at:      0      76     402 
   Size:         76     326
 
Bsp1286I G_dGCh'C

Cuts at:      0     287     338     359     402 
   Size:        287      51      21      43

  Fragments arranged by size: 

                 287      51      43      21
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     170     402 
   Size:        170     232
 
BssHII G'CGCG_C

Cuts at:      0      23     402 
   Size:         23     379
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     348     402 
   Size:        348      54
 
BstYI r'GATC_y

Cuts at:      0     115     325     402 
   Size:        115     210      77

  Fragments arranged by size: 

                 210     115      77
 
Bsu36I CC'TnA_GG

Cuts at:      0     110     402 
   Size:        110     292
 
BtgI C'CryG_G

Cuts at:      0     359     402 
   Size:        359      43
 
BtsI GCAGTG_nn'

Cuts at:      0     359     402 
   Size:        359      43
 
ClaI AT'CG_AT

Cuts at:      0     212     402 
   Size:        212     190
 
EaeI y'GGCC_r

Cuts at:      0      73     402 
   Size:         73     329
 
EagI C'GGCC_G

Cuts at:      0      73     402 
   Size:         73     329
 
EarI CTCTTCn'nnn_

Cuts at:      0     293     402 
   Size:        293     109
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     319     402 
   Size:        319      83
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     286     402 
   Size:        247      39     116

  Fragments arranged by size: 

                 247     116      39
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     198     402 
   Size:        198     204
 
EcoO109I rG'GnC_Cy

Cuts at:      0     378     402 
   Size:        378      24
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     252     402 
   Size:        252     150
 
MslI CAynn'nnrTG

Cuts at:      0     364     402 
   Size:        364      38
 
MspA1I CmG'CkG

Cuts at:      0     185     273     316     402 
   Size:        185      88      43      86

  Fragments arranged by size: 

                 185      88      86      43
 
PpuMI rG'GwC_Cy

Cuts at:      0     378     402 
   Size:        378      24
 
PvuII CAG'CTG

Cuts at:      0     273     402 
   Size:        273     129
 
SfcI C'TryA_G

Cuts at:      0     288     402 
   Size:        288     114
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     226     258     317     349     402 
   Size:        226      32      59      32      53

  Fragments arranged by size: 

                 226      59      53      32      32
 

 Enzymes that do cut:

     AcuI     AleI    BamHI    BanII     BbsI    BciVI     BglI     BlpI
 Bme1580I    BseYI     BsgI    BsiEI Bsp1286I    BspMI   BssHII    BstXI
    BstYI   Bsu36I     BtgI     BtsI     ClaI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI EcoO109I     FalI     MslI   MspA1I    PpuMI
    PvuII     SfcI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI
    BbvCI     BcgI     BclI    BfrBI    BglII    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
   BstEII  BstZ17I    BtgZI    CspCI     DraI   DraIII     DrdI  EcoICRI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI