Restriction map for
pF1KB7894
(Linear) (Six-base) MAPSORT of: KIBB7894.seq Check: 1975 from: 1 to: 1968
KIBB7894.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AatII G_ACGT'C
Cuts at: 0 1540 1968
Size: 1540 428
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1286 1315 1968
Size: 1286 29 653
Fragments arranged by size:
1286 653 29
AfeI AGC'GCT
Cuts at: 0 1510 1968
Size: 1510 458
AflIII A'CryG_T
Cuts at: 0 1485 1968
Size: 1485 483
AhdI GACnn_n'nnGTC
Cuts at: 0 51 1107 1968
Size: 51 1056 861
Fragments arranged by size:
1056 861 51
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1300 1331 1968
Size: 1300 31 637
Fragments arranged by size:
1300 637 31
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1334 1366 1968
Size: 1334 32 602
Fragments arranged by size:
1334 602 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1125 1641 1968
Size: 1125 516 327
Fragments arranged by size:
1125 516 327
ApaI G_GGCC'C
Cuts at: 0 586 636 1133 1968
Size: 586 50 497 835
Fragments arranged by size:
835 586 497 50
AvaI C'yCGr_G
Cuts at: 0 305 398 635 1648 1968
Size: 305 93 237 1013 320
Fragments arranged by size:
1013 320 305 237 93
BamHI G'GATC_C
Cuts at: 0 858 1968
Size: 858 1110
BanI G'GyrC_C
Cuts at: 0 1469 1694 1968
Size: 1469 225 274
Fragments arranged by size:
1469 274 225
BanII G_rGCy'C
Cuts at: 0 305 406 586 636 1133 1255 1724 1968
Size: 305 101 180 50 497 122 469 244
Fragments arranged by size:
497 469 305 244 180 122 101 50
BglI GCCn_nnn'nGGC
Cuts at: 0 708 1968
Size: 708 1260
BglII A'GATC_T
Cuts at: 0 1448 1968
Size: 1448 520
Bme1580I G_kGCm'C
Cuts at: 0 586 636 1133 1320 1472 1648 1699 1968
Size: 586 50 497 187 152 176 51 269
Fragments arranged by size:
586 497 269 187 176 152 51 50
BmrI ACTGGGnnnn_n'
Cuts at: 0 1113 1968
Size: 1113 855
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 852 1968
Size: 852 1116
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 14 1618 1938 1968
Size: 14 1604 320 30
Fragments arranged by size:
1604 320 30 14
Bpu10I CC'TnA_GC
Cuts at: 0 827 1968
Size: 827 1141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1381 1968
Size: 1381 587
BsaAI yAC'GTr
Cuts at: 0 1378 1486 1708 1968
Size: 1378 108 222 260
Fragments arranged by size:
1378 260 222 108
BsaHI Gr'CG_yC
Cuts at: 0 679 784 1537 1968
Size: 679 105 753 431
Fragments arranged by size:
753 679 431 105
BsaWI w'CCGG_w
Cuts at: 0 99 164 1968
Size: 99 65 1804
Fragments arranged by size:
1804 99 65
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 991 1021 1936 1966 1968
Size: 991 30 915 30 2
Fragments arranged by size:
991 915 30 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1039 1423 1968
Size: 1039 384 545
Fragments arranged by size:
1039 545 384
BseYI C'CCAG_C
Cuts at: 0 451 1151 1156 1226 1775 1968
Size: 451 700 5 70 549 193
Fragments arranged by size:
700 549 451 193 70 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 905 1894 1968
Size: 237 668 989 74
Fragments arranged by size:
989 668 237 74
BsiEI CG_ry'CG
Cuts at: 0 1965 1968
Size: 1965 3
BsiHKAI G_wGCw'C
Cuts at: 0 1274 1655 1724 1968
Size: 1274 381 69 244
Fragments arranged by size:
1274 381 244 69
BsmI GAATG_Cn'
Cuts at: 0 75 1295 1968
Size: 75 1220 673
Fragments arranged by size:
1220 673 75
Bsp1286I G_dGCh'C
Cuts at: 0 305 406 586 636 1133 1255 1274 1320
Size: 305 101 180 50 497 122 19 46
Cuts at: 1320 1472 1648 1655 1699 1724 1968
Size: 152 176 7 44 25 244
Fragments arranged by size:
497 305 244 180 176 152 122 101
50 46 44 25 19 7
BspEI T'CCGG_A
Cuts at: 0 164 1968
Size: 164 1804
BspHI T'CATG_A
Cuts at: 0 116 1968
Size: 116 1852
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 359 1451 1955 1968
Size: 359 1092 504 13
Fragments arranged by size:
1092 504 359 13
BsrBI CCG'CTC
Cuts at: 0 273 288 1968
Size: 273 15 1680
Fragments arranged by size:
1680 273 15
BsrDI GCAATG_nn'
Cuts at: 0 144 288 1259 1866 1968
Size: 144 144 971 607 102
Fragments arranged by size:
971 607 144 144 102
BsrFI r'CCGG_y
Cuts at: 0 608 707 1968
Size: 608 99 1261
Fragments arranged by size:
1261 608 99
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1395 1968
Size: 1395 573
BstYI r'GATC_y
Cuts at: 0 593 858 1217 1448 1968
Size: 593 265 359 231 520
Fragments arranged by size:
593 520 359 265 231
Bsu36I CC'TnA_GG
Cuts at: 0 809 1968
Size: 809 1159
BtgI C'CryG_G
Cuts at: 0 150 903 1968
Size: 150 753 1065
Fragments arranged by size:
1065 753 150
BtsI GCAGTG_nn'
Cuts at: 0 303 712 785 977 1111 1246 1968
Size: 303 409 73 192 134 135 722
Fragments arranged by size:
722 409 303 192 135 134 73
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1548 1583 1968
Size: 1548 35 385
Fragments arranged by size:
1548 385 35
EaeI y'GGCC_r
Cuts at: 0 182 443 709 1968
Size: 182 261 266 1259
Fragments arranged by size:
1259 266 261 182
EarI CTCTTCn'nnn_
Cuts at: 0 926 958 1262 1591 1968
Size: 926 32 304 329 377
Fragments arranged by size:
926 377 329 304 32
EcoICRI GAG'CTC
Cuts at: 0 1722 1968
Size: 1722 246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 14 1286 1315 1618 1938 1968
Size: 14 1272 29 303 320 30
Fragments arranged by size:
1272 320 303 30 29 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 871 1968
Size: 871 1097
EcoO109I rG'GnC_Cy
Cuts at: 0 582 583 701 846 890 1101 1129 1662
Size: 582 1 118 145 44 211 28 533
Cuts at: 1662 1968
Size: 306
Fragments arranged by size:
582 533 306 211 145 118 44 28
1
FspI TGC'GCA
Cuts at: 0 1867 1968
Size: 1867 101
FspAI rTGC'GCAy
Cuts at: 0 1867 1968
Size: 1867 101
HaeII r_GCGC'y
Cuts at: 0 360 1512 1755 1968
Size: 360 1152 243 213
Fragments arranged by size:
1152 360 243 213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 234 266 991 1023 1093 1125
Size: 37 32 165 32 725 32 70 32
Cuts at: 1125 1936 1968
Size: 811 32
Fragments arranged by size:
811 725 165 70 37 32 32 32
32 32
MscI TGG'CCA
Cuts at: 0 184 445 1968
Size: 184 261 1523
Fragments arranged by size:
1523 261 184
MslI CAynn'nnrTG
Cuts at: 0 1873 1968
Size: 1873 95
MspA1I CmG'CkG
Cuts at: 0 627 1678 1968
Size: 627 1051 290
Fragments arranged by size:
1051 627 290
NaeI GCC'GGC
Cuts at: 0 610 709 1968
Size: 610 99 1259
Fragments arranged by size:
1259 610 99
NcoI C'CATG_G
Cuts at: 0 150 903 1968
Size: 150 753 1065
Fragments arranged by size:
1065 753 150
NgoMIV G'CCGG_C
Cuts at: 0 608 707 1968
Size: 608 99 1261
Fragments arranged by size:
1261 608 99
NspI r_CATG'y
Cuts at: 0 504 524 988 1968
Size: 504 20 464 980
Fragments arranged by size:
980 504 464 20
PflMI CCAn_nnn'nTGG
Cuts at: 0 1125 1968
Size: 1125 843
PfoI T'CCnGG_A
Cuts at: 0 194 944 1968
Size: 194 750 1024
Fragments arranged by size:
1024 750 194
PmlI CAC'GTG
Cuts at: 0 1486 1968
Size: 1486 482
PpuMI rG'GwC_Cy
Cuts at: 0 846 1101 1662 1968
Size: 846 255 561 306
Fragments arranged by size:
846 561 306 255
PshAI GACnn'nnGTC
Cuts at: 0 542 1968
Size: 542 1426
PspOMI G'GGCC_C
Cuts at: 0 582 632 1129 1968
Size: 582 50 497 839
Fragments arranged by size:
839 582 497 50
PstI C_TGCA'G
Cuts at: 0 356 1448 1968
Size: 356 1092 520
Fragments arranged by size:
1092 520 356
PvuII CAG'CTG
Cuts at: 0 627 1678 1968
Size: 627 1051 290
Fragments arranged by size:
1051 627 290
SacI G_AGCT'C
Cuts at: 0 1724 1968
Size: 1724 244
SanDI GG'GwC_CC
Cuts at: 0 1662 1968
Size: 1662 306
SapI GCTCTTCn'nnn_
Cuts at: 0 1262 1968
Size: 1262 706
ScaI AGT'ACT
Cuts at: 0 652 1968
Size: 652 1316
SfcI C'TryA_G
Cuts at: 0 352 815 1444 1968
Size: 352 463 629 524
Fragments arranged by size:
629 524 463 352
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 708 1968
Size: 708 1260
SmaI CCC'GGG
Cuts at: 0 307 637 1968
Size: 307 330 1331
Fragments arranged by size:
1331 330 307
SmlI C'TyrA_G
Cuts at: 0 1360 1968
Size: 1360 608
StuI AGG'CCT
Cuts at: 0 333 1968
Size: 333 1635
StyI C'CwwG_G
Cuts at: 0 150 185 437 697 903 1097 1968
Size: 150 35 252 260 206 194 871
Fragments arranged by size:
871 260 252 206 194 150 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 465 736 1094 1968
Size: 465 271 358 874
Fragments arranged by size:
874 465 358 271
TatI w'GTAC_w
Cuts at: 0 650 1968
Size: 650 1318
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1540 1968
Size: 1540 428
XmaI C'CCGG_G
Cuts at: 0 305 635 1968
Size: 305 330 1333
Fragments arranged by size:
1333 330 305
XmnI GAAnn'nnTTC
Cuts at: 0 574 1295 1968
Size: 574 721 673
Fragments arranged by size:
721 673 574
ZraI GAC'GTC
Cuts at: 0 1538 1968
Size: 1538 430
Enzymes that do cut:
AatII AcuI AfeI AflIII AhdI AlfI AloI AlwNI
ApaI AvaI BamHI BanI BanII BglI BglII Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstYI
Bsu36I BtgI BtsI CspCI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FspI FspAI HaeII Hin4I MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PflMI PfoI PmlI
PpuMI PshAI PspOMI PstI PvuII SacI SanDI SapI
ScaI SfcI SfiI SmaI SmlI StuI StyI TaqII
TatI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AgeI AleI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI BmtI
BsaI BsaBI BsiWI BsmBI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I BtgZI ClaI DraI DraIII DrdI
EagI EciI EcoRI EcoRV FalI FseI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NarI NdeI
NheI NotI NruI NsiI PacI PasI PciI PmeI
PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI
SbfI SexAI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XhoI