Restriction map for
pF1KB7890
(Linear) (Six-base) MAPSORT of: KIBB7890.seq Check: 1850 from: 1 to: 1956
KIBB7890.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AclI AA'CG_TT
Cuts at: 0 1689 1956
Size: 1689 267
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 1117 1472 1631 1928 1956
Size: 739 378 355 159 297 28
Fragments arranged by size:
739 378 355 297 159 28
AfeI AGC'GCT
Cuts at: 0 1562 1956
Size: 1562 394
AhdI GACnn_n'nnGTC
Cuts at: 0 570 1956
Size: 570 1386
AleI CACnn'nnGTG
Cuts at: 0 1192 1956
Size: 1192 764
AlwNI CAG_nnn'CTG
Cuts at: 0 717 1956
Size: 717 1239
ApaI G_GGCC'C
Cuts at: 0 1217 1956
Size: 1217 739
ApoI r'AATT_y
Cuts at: 0 689 707 1015 1578 1956
Size: 689 18 308 563 378
Fragments arranged by size:
689 563 378 308 18
AvaI C'yCGr_G
Cuts at: 0 123 1956
Size: 123 1833
BamHI G'GATC_C
Cuts at: 0 1071 1956
Size: 1071 885
BanII G_rGCy'C
Cuts at: 0 1217 1956
Size: 1217 739
BbsI GAAGACnn'nnnn_
Cuts at: 0 155 1134 1310 1907 1956
Size: 155 979 176 597 49
Fragments arranged by size:
979 597 176 155 49
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 739 773 1956
Size: 739 34 1183
Fragments arranged by size:
1183 739 34
BclI T'GATC_A
Cuts at: 0 509 1956
Size: 509 1447
BfrBI ATG'CAT
Cuts at: 0 1752 1956
Size: 1752 204
BglI GCCn_nnn'nGGC
Cuts at: 0 1524 1956
Size: 1524 432
BglII A'GATC_T
Cuts at: 0 309 1956
Size: 309 1647
Bme1580I G_kGCm'C
Cuts at: 0 131 1217 1956
Size: 131 1086 739
Fragments arranged by size:
1086 739 131
BmrI ACTGGGnnnn_n'
Cuts at: 0 1120 1956
Size: 1120 836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 298 857 995 1160 1175 1521 1654
Size: 120 178 559 138 165 15 346 133
Cuts at: 1654 1790 1956
Size: 136 166
Fragments arranged by size:
559 346 178 166 165 138 136 133
120 15
Bpu10I CC'TnA_GC
Cuts at: 0 1660 1956
Size: 1660 296
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1529 1956
Size: 1529 427
BsaI GGTCTCn'nnnn_
Cuts at: 0 275 634 1639 1956
Size: 275 359 1005 317
Fragments arranged by size:
1005 359 317 275
BsaAI yAC'GTr
Cuts at: 0 1352 1826 1956
Size: 1352 474 130
Fragments arranged by size:
1352 474 130
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 1956
Size: 61 1895
BseYI C'CCAG_C
Cuts at: 0 868 1956
Size: 868 1088
BsiHKAI G_wGCw'C
Cuts at: 0 1699 1956
Size: 1699 257
BsmI GAATG_Cn'
Cuts at: 0 1413 1956
Size: 1413 543
Bsp1286I G_dGCh'C
Cuts at: 0 131 1217 1699 1956
Size: 131 1086 482 257
Fragments arranged by size:
1086 482 257 131
BspHI T'CATG_A
Cuts at: 0 162 1956
Size: 162 1794
BsrDI GCAATG_nn'
Cuts at: 0 1444 1850 1956
Size: 1444 406 106
Fragments arranged by size:
1444 406 106
BstBI TT'CG_AA
Cuts at: 0 146 1836 1956
Size: 146 1690 120
Fragments arranged by size:
1690 146 120
BstYI r'GATC_y
Cuts at: 0 309 450 1071 1956
Size: 309 141 621 885
Fragments arranged by size:
885 621 309 141
BtgI C'CryG_G
Cuts at: 0 113 1956
Size: 113 1843
DraI TTT'AAA
Cuts at: 0 918 1956
Size: 918 1038
DraIII CAC_nnn'GTG
Cuts at: 0 793 1956
Size: 793 1163
EaeI y'GGCC_r
Cuts at: 0 1346 1956
Size: 1346 610
EarI CTCTTCn'nnn_
Cuts at: 0 148 243 1956
Size: 148 95 1713
Fragments arranged by size:
1713 148 95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 298 739 857 995 1117 1160 1175
Size: 120 178 441 118 138 122 43 15
Cuts at: 1175 1472 1521 1631 1654 1790 1928 1956
Size: 297 49 110 23 136 138 28
Fragments arranged by size:
441 297 178 138 138 136 122 120
118 110 49 43 28 23 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 43 1956
Size: 43 1913
EcoO109I rG'GnC_Cy
Cuts at: 0 1214 1421 1732 1956
Size: 1214 207 311 224
Fragments arranged by size:
1214 311 224 207
EcoRI G'AATT_C
Cuts at: 0 707 1956
Size: 707 1249
EcoRV GAT'ATC
Cuts at: 0 1815 1956
Size: 1815 141
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 612 919 1956
Size: 612 307 1037
Fragments arranged by size:
1037 612 307
HaeII r_GCGC'y
Cuts at: 0 1564 1956
Size: 1564 392
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 435 467 556 588 1761 1793 1956
Size: 435 32 89 32 1173 32 163
Fragments arranged by size:
1173 435 163 89 32 32 32
HindIII A'AGCT_T
Cuts at: 0 413 1956
Size: 413 1543
MfeI C'AATT_G
Cuts at: 0 436 1956
Size: 436 1520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 1956
Size: 8 1948
MscI TGG'CCA
Cuts at: 0 1348 1956
Size: 1348 608
MslI CAynn'nnrTG
Cuts at: 0 1192 1956
Size: 1192 764
NsiI A_TGCA'T
Cuts at: 0 1754 1956
Size: 1754 202
PflMI CCAn_nnn'nTGG
Cuts at: 0 1068 1956
Size: 1068 888
PfoI T'CCnGG_A
Cuts at: 0 1417 1670 1956
Size: 1417 253 286
Fragments arranged by size:
1417 286 253
PmlI CAC'GTG
Cuts at: 0 1352 1956
Size: 1352 604
PpuMI rG'GwC_Cy
Cuts at: 0 1421 1732 1956
Size: 1421 311 224
Fragments arranged by size:
1421 311 224
PshAI GACnn'nnGTC
Cuts at: 0 1134 1956
Size: 1134 822
PsiI TTA'TAA
Cuts at: 0 653 1956
Size: 653 1303
PspOMI G'GGCC_C
Cuts at: 0 1213 1956
Size: 1213 743
SanDI GG'GwC_CC
Cuts at: 0 1732 1956
Size: 1732 224
ScaI AGT'ACT
Cuts at: 0 1542 1956
Size: 1542 414
SexAI A'CCwGG_T
Cuts at: 0 1944 1956
Size: 1944 12
SfcI C'TryA_G
Cuts at: 0 1218 1956
Size: 1218 738
SmlI C'TyrA_G
Cuts at: 0 1544 1956
Size: 1544 412
SspI AAT'ATT
Cuts at: 0 1625 1956
Size: 1625 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 536 1956
Size: 536 1420
TatI w'GTAC_w
Cuts at: 0 610 1540 1956
Size: 610 930 416
Fragments arranged by size:
930 610 416
XmnI GAAnn'nnTTC
Cuts at: 0 707 1956
Size: 707 1249
Enzymes that do cut:
AclI AcuI AfeI AhdI AleI AlwNI ApaI ApoI
AvaI BamHI BanII BbsI BcgI BclI BfrBI BglI
BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BsrDI BstBI
BstYI BtgI DraI DraIII EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HindIII MfeI
MmeI MscI MslI NsiI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI SanDI ScaI SexAI SfcI SmlI
SspI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AflII AflIII AgeI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BanI
BbeI BbvCI BciVI BlpI BmgBI BmtI BplI BsaBI
BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DrdI
EagI EciI EcoICRI FseI FspI FspAI HincII HpaI
KasI KpnI MluI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PmeI PpiI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI StyI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI