Restriction map for
pF1KB7879
(Linear) (Six-base) MAPSORT of: KIBB7879.seq Check: 2429 from: 1 to: 1725
KIBB7879.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 241 1725
Size: 241 1484
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 645 747 1725
Size: 645 102 978
Fragments arranged by size:
978 645 102
AleI CACnn'nnGTG
Cuts at: 0 278 1725
Size: 278 1447
AlwNI CAG_nnn'CTG
Cuts at: 0 10 354 1725
Size: 10 344 1371
Fragments arranged by size:
1371 344 10
ApoI r'AATT_y
Cuts at: 0 209 436 1619 1725
Size: 209 227 1183 106
Fragments arranged by size:
1183 227 209 106
AvrII C'CTAG_G
Cuts at: 0 244 1725
Size: 244 1481
BanI G'GyrC_C
Cuts at: 0 20 192 1725
Size: 20 172 1533
Fragments arranged by size:
1533 172 20
BbeI G_GCGC'C
Cuts at: 0 24 1725
Size: 24 1701
BbsI GAAGACnn'nnnn_
Cuts at: 0 82 504 586 1725
Size: 82 422 82 1139
Fragments arranged by size:
1139 422 82 82
BfrBI ATG'CAT
Cuts at: 0 600 1725
Size: 600 1125
Bme1580I G_kGCm'C
Cuts at: 0 40 1725
Size: 40 1685
BmrI ACTGGGnnnn_n'
Cuts at: 0 536 1301 1725
Size: 536 765 424
Fragments arranged by size:
765 536 424
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 257 1725
Size: 257 1468
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 1061 1145 1229 1397 1481 1649 1725
Size: 977 84 84 84 168 84 168 76
Fragments arranged by size:
977 168 168 84 84 84 84 76
Bpu10I CC'TnA_GC
Cuts at: 0 487 1725
Size: 487 1238
BsaHI Gr'CG_yC
Cuts at: 0 21 1725
Size: 21 1704
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 70 100 1725
Size: 70 30 1625
Fragments arranged by size:
1625 70 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 202 910 1725
Size: 94 108 708 815
Fragments arranged by size:
815 708 108 94
BseYI C'CCAG_C
Cuts at: 0 159 1725
Size: 159 1566
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 744 1584 1725
Size: 744 840 141
Fragments arranged by size:
840 744 141
BsmI GAATG_Cn'
Cuts at: 0 1483 1651 1725
Size: 1483 168 74
Fragments arranged by size:
1483 168 74
Bsp1286I G_dGCh'C
Cuts at: 0 40 1725
Size: 40 1685
BspHI T'CATG_A
Cuts at: 0 1359 1725
Size: 1359 366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 241 1725
Size: 241 1484
BsrGI T'GTAC_A
Cuts at: 0 216 1725
Size: 216 1509
BstEII G'GTnAC_C
Cuts at: 0 1009 1725
Size: 1009 716
BstYI r'GATC_y
Cuts at: 0 1258 1725
Size: 1258 467
BtsI GCAGTG_nn'
Cuts at: 0 392 732 785 1488 1572 1725
Size: 392 340 53 703 84 153
Fragments arranged by size:
703 392 340 153 84 53
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1218 1253 1725
Size: 1218 35 472
Fragments arranged by size:
1218 472 35
DraIII CAC_nnn'GTG
Cuts at: 0 1434 1725
Size: 1434 291
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 645 747 977 1061 1145 1229 1397 1481
Size: 645 102 230 84 84 84 168 84
Cuts at: 1481 1649 1725
Size: 168 76
Fragments arranged by size:
645 230 168 168 102 84 84 84
84 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 248 1725
Size: 248 1477
EcoO109I rG'GnC_Cy
Cuts at: 0 796 964 1217 1725
Size: 796 168 253 508
Fragments arranged by size:
796 508 253 168
EcoRI G'AATT_C
Cuts at: 0 1619 1725
Size: 1619 106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1113 1725
Size: 1113 612
HaeII r_GCGC'y
Cuts at: 0 24 1725
Size: 24 1701
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 870 902 1038 1070 1250 1282 1428 1460
Size: 870 32 136 32 180 32 146 32
Cuts at: 1460 1725
Size: 265
Fragments arranged by size:
870 265 180 146 136 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 20 1725
Size: 20 1705
MfeI C'AATT_G
Cuts at: 0 1308 1725
Size: 1308 417
MslI CAynn'nnrTG
Cuts at: 0 278 1112 1392 1725
Size: 278 834 280 333
Fragments arranged by size:
834 333 280 278
MspA1I CmG'CkG
Cuts at: 0 7 1725
Size: 7 1718
NarI GG'CG_CC
Cuts at: 0 21 1725
Size: 21 1704
NdeI CA'TA_TG
Cuts at: 0 1389 1725
Size: 1389 336
NsiI A_TGCA'T
Cuts at: 0 602 1725
Size: 602 1123
NspI r_CATG'y
Cuts at: 0 374 1725
Size: 374 1351
PpuMI rG'GwC_Cy
Cuts at: 0 796 1725
Size: 796 929
PsiI TTA'TAA
Cuts at: 0 1559 1725
Size: 1559 166
SfcI C'TryA_G
Cuts at: 0 441 572 1725
Size: 441 131 1153
Fragments arranged by size:
1153 441 131
SfoI GGC'GCC
Cuts at: 0 22 1725
Size: 22 1703
StuI AGG'CCT
Cuts at: 0 1134 1638 1720 1725
Size: 1134 504 82 5
Fragments arranged by size:
1134 504 82 5
StyI C'CwwG_G
Cuts at: 0 244 1725
Size: 244 1481
TatI w'GTAC_w
Cuts at: 0 216 1725
Size: 216 1509
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 70 102 704 736 1725
Size: 70 32 602 32 989
Fragments arranged by size:
989 602 70 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 697 1725
Size: 697 1028
Enzymes that do cut:
AarI AcuI AleI AlwNI ApoI AvrII BanI BbeI
BbsI BfrBI Bme1580I BmrI BplI BpmI Bpu10I BsaHI
BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspHI BspMI
BsrGI BstEII BstYI BtsI CspCI DraIII Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I KasI MfeI MslI
MspA1I NarI NdeI NsiI NspI PpuMI PsiI SfcI
SfoI StuI StyI TatI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI BaeI BamHI BanII BbvCI BcgI BciVI BclI
BglI BglII BlpI BmgBI BmtI BpuEI BsaI BsaAI
BsaBI BsaWI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstZ17I
Bsu36I BtgI BtgZI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KpnI MluI MmeI MscI NaeI NcoI
NgoMIV NheI NotI NruI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI ZraI