Restriction map for
pF1KB7876
(Linear) (Six-base) MAPSORT of: KIBB7876.seq Check: 52 from: 1 to: 1914
KIBB7876.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 750 1170 1506 1914
Size: 196 554 420 336 408
Fragments arranged by size:
554 420 408 336 196
ApoI r'AATT_y
Cuts at: 0 146 496 1544 1914
Size: 146 350 1048 370
Fragments arranged by size:
1048 370 350 146
BanII G_rGCy'C
Cuts at: 0 384 1453 1896 1914
Size: 384 1069 443 18
Fragments arranged by size:
1069 443 384 18
BfrBI ATG'CAT
Cuts at: 0 1246 1632 1716 1914
Size: 1246 386 84 198
Fragments arranged by size:
1246 386 198 84
BglII A'GATC_T
Cuts at: 0 1189 1525 1914
Size: 1189 336 389
Fragments arranged by size:
1189 389 336
BmrI ACTGGGnnnn_n'
Cuts at: 0 650 734 854 1022 1358 1914
Size: 650 84 120 168 336 556
Fragments arranged by size:
650 556 336 168 120 84
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 127 1914
Size: 127 1787
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 578 998 1082 1166 1418 1502 1586
Size: 212 366 420 84 84 252 84 84
Cuts at: 1586 1670 1754 1838 1914
Size: 84 84 84 76
Fragments arranged by size:
420 366 252 212 84 84 84 84
84 84 84 76
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 340 370 1914
Size: 340 30 1544
Fragments arranged by size:
1544 340 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 348 1914
Size: 348 1566
BsiHKAI G_wGCw'C
Cuts at: 0 240 1453 1914
Size: 240 1213 461
Fragments arranged by size:
1213 461 240
BsmI GAATG_Cn'
Cuts at: 0 421 679 847 974 1420 1504 1914
Size: 421 258 168 127 446 84 410
Fragments arranged by size:
446 421 410 258 168 127 84
Bsp1286I G_dGCh'C
Cuts at: 0 240 384 1453 1896 1914
Size: 240 144 1069 443 18
Fragments arranged by size:
1069 443 240 144 18
BspHI T'CATG_A
Cuts at: 0 1128 1914
Size: 1128 786
BsrDI GCAATG_nn'
Cuts at: 0 1177 1513 1765 1914
Size: 1177 336 252 149
Fragments arranged by size:
1177 336 252 149
BstBI TT'CG_AA
Cuts at: 0 618 1794 1914
Size: 618 1176 120
Fragments arranged by size:
1176 618 120
BstYI r'GATC_y
Cuts at: 0 1189 1432 1525 1609 1914
Size: 1189 243 93 84 305
Fragments arranged by size:
1189 305 243 93 84
BtgI C'CryG_G
Cuts at: 0 471 1914
Size: 471 1443
BtsI GCAGTG_nn'
Cuts at: 0 219 240 1429 1597 1681 1896 1914
Size: 219 21 1189 168 84 215 18
Fragments arranged by size:
1189 219 215 168 84 21 18
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1071 1106 1407 1442 1491 1526 1914
Size: 1071 35 301 35 49 35 388
Fragments arranged by size:
1071 388 301 49 35 35 35
EarI CTCTTCn'nnn_
Cuts at: 0 777 1310 1914
Size: 777 533 604
Fragments arranged by size:
777 604 533
EcoICRI GAG'CTC
Cuts at: 0 1451 1914
Size: 1451 463
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 212 578 750 998 1082 1166 1170
Size: 196 16 366 172 248 84 84 4
Cuts at: 1170 1418 1502 1506 1586 1670 1754 1838 1914
Size: 248 84 4 80 84 84 84 76
Fragments arranged by size:
366 248 248 196 172 84 84 84
84 84 84 80 76 16 4 4
EcoRV GAT'ATC
Cuts at: 0 986 1126 1462 1914
Size: 986 140 336 452
Fragments arranged by size:
986 452 336 140
HindIII A'AGCT_T
Cuts at: 0 1769 1914
Size: 1769 145
MslI CAynn'nnrTG
Cuts at: 0 137 321 573 741 825 852 1379 1914
Size: 137 184 252 168 84 27 527 535
Fragments arranged by size:
535 527 252 184 168 137 84 27
NdeI CA'TA_TG
Cuts at: 0 288 738 822 1914
Size: 288 450 84 1092
Fragments arranged by size:
1092 450 288 84
NruI TCG'CGA
Cuts at: 0 1704 1914
Size: 1704 210
NsiI A_TGCA'T
Cuts at: 0 1248 1634 1718 1914
Size: 1248 386 84 196
Fragments arranged by size:
1248 386 196 84
NspI r_CATG'y
Cuts at: 0 174 698 1250 1914
Size: 174 524 552 664
Fragments arranged by size:
664 552 524 174
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 1914
Size: 10 32 1872
Fragments arranged by size:
1872 32 10
PsiI TTA'TAA
Cuts at: 0 488 656 1914
Size: 488 168 1258
Fragments arranged by size:
1258 488 168
SacI G_AGCT'C
Cuts at: 0 1453 1914
Size: 1453 461
SapI GCTCTTCn'nnn_
Cuts at: 0 777 1914
Size: 777 1137
SfcI C'TryA_G
Cuts at: 0 29 1914
Size: 29 1885
XmnI GAAnn'nnTTC
Cuts at: 0 679 763 1183 1351 1914
Size: 679 84 420 168 563
Fragments arranged by size:
679 563 420 168 84
Enzymes that do cut:
AcuI ApoI BanII BfrBI BglII BmrI BplI BpmI
BsaXI BseRI BsiHKAI BsmI Bsp1286I BspHI BsrDI BstBI
BstYI BtgI BtsI CspCI EarI EcoICRI Eco57MI EcoRV
HindIII MslI NdeI NruI NsiI NspI PpiI PsiI
SacI SapI SfcI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BbsI BbvCI BcgI BciVI BclI BglI BlpI
Bme1580I BmgBI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiWI BsmBI BspEI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI
EaeI EagI EciI EcoNI EcoO109I EcoRI FalI FseI
FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI MscI MspA1I NaeI NarI NcoI
NgoMIV NheI NotI PacI PasI PciI PflMI PfoI
PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI