Restriction map for
pF1KB7875
(Linear) (Six-base) MAPSORT of: KIBB7875.seq Check: 3925 from: 1 to: 1710
KIBB7875.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 1710
Size: 31 1679
AgeI A'CCGG_T
Cuts at: 0 896 980 1400 1710
Size: 896 84 420 310
Fragments arranged by size:
896 420 310 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 63 1710
Size: 63 1647
ApoI r'AATT_y
Cuts at: 0 127 562 674 690 758 926 1195 1262
Size: 127 435 112 16 68 168 269 67
Cuts at: 1262 1710
Size: 448
Fragments arranged by size:
448 435 269 168 127 112 68 67
16
BanII G_rGCy'C
Cuts at: 0 281 1710
Size: 281 1429
BbsI GAAGACnn'nnnn_
Cuts at: 0 207 1565 1710
Size: 207 1358 145
Fragments arranged by size:
1358 207 145
BciVI GTATCCnnnnn_n'
Cuts at: 0 35 1710
Size: 35 1675
BclI T'GATC_A
Cuts at: 0 251 1710
Size: 251 1459
BlpI GC'TnA_GC
Cuts at: 0 164 1710
Size: 164 1546
BmrI ACTGGGnnnn_n'
Cuts at: 0 313 1710
Size: 313 1397
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 157 788 872 956 1040 1124 1208
Size: 38 119 631 84 84 84 84 84
Cuts at: 1208 1292 1376 1460 1628 1710
Size: 84 84 84 168 82
Fragments arranged by size:
631 168 119 84 84 84 84 84
84 84 84 82 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 1710
Size: 16 1694
BsaWI w'CCGG_w
Cuts at: 0 896 980 1400 1710
Size: 896 84 420 310
Fragments arranged by size:
896 420 310 84
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 1710
Size: 154 1556
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 1319 1403 1710
Size: 454 865 84 307
Fragments arranged by size:
865 454 307 84
Bsp1286I G_dGCh'C
Cuts at: 0 281 1710
Size: 281 1429
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 214 1710
Size: 214 1496
BsrDI GCAATG_nn'
Cuts at: 0 154 1710
Size: 154 1556
BsrFI r'CCGG_y
Cuts at: 0 896 980 1400 1710
Size: 896 84 420 310
Fragments arranged by size:
896 420 310 84
BstAPI GCAn_nnn'nTGC
Cuts at: 0 229 1710
Size: 229 1481
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 316 1710
Size: 316 1394
BtsI GCAGTG_nn'
Cuts at: 0 158 1710
Size: 158 1552
EaeI y'GGCC_r
Cuts at: 0 119 352 1710
Size: 119 233 1358
Fragments arranged by size:
1358 233 119
EarI CTCTTCn'nnn_
Cuts at: 0 286 1710
Size: 286 1424
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 38 157 788 872 956 1040 1124
Size: 31 7 119 631 84 84 84 84
Cuts at: 1124 1208 1292 1376 1460 1628 1710
Size: 84 84 84 84 168 82
Fragments arranged by size:
631 168 119 84 84 84 84 84
84 84 84 82 31 7
EcoRI G'AATT_C
Cuts at: 0 674 758 926 1262 1710
Size: 674 84 168 336 448
Fragments arranged by size:
674 448 336 168 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 243 275 294 326 1710
Size: 243 32 19 32 1384
Fragments arranged by size:
1384 243 32 32 19
HindIII A'AGCT_T
Cuts at: 0 803 1055 1139 1307 1475 1643 1710
Size: 803 252 84 168 168 168 67
Fragments arranged by size:
803 252 168 168 168 84 67
MscI TGG'CCA
Cuts at: 0 121 354 1710
Size: 121 233 1356
Fragments arranged by size:
1356 233 121
NspI r_CATG'y
Cuts at: 0 301 648 1710
Size: 301 347 1062
Fragments arranged by size:
1062 347 301
PasI CC'CwG_GG
Cuts at: 0 52 1710
Size: 52 1658
PsiI TTA'TAA
Cuts at: 0 560 1710
Size: 560 1150
SexAI A'CCwGG_T
Cuts at: 0 209 1710
Size: 209 1501
SfcI C'TryA_G
Cuts at: 0 201 864 948 1710
Size: 201 663 84 762
Fragments arranged by size:
762 663 201 84
SmlI C'TyrA_G
Cuts at: 0 31 1710
Size: 31 1679
SphI G_CATG'C
Cuts at: 0 648 1710
Size: 648 1062
SspI AAT'ATT
Cuts at: 0 527 1710
Size: 527 1183
TatI w'GTAC_w
Cuts at: 0 77 1710
Size: 77 1633
XbaI T'CTAG_A
Cuts at: 0 261 1710
Size: 261 1449
XmnI GAAnn'nnTTC
Cuts at: 0 527 610 1710
Size: 527 83 1100
Fragments arranged by size:
1100 527 83
Enzymes that do cut:
AcuI AgeI AlfI ApoI BanII BbsI BciVI BclI
BlpI BmrI BpmI BpuEI BsaWI BseRI BsgI Bsp1286I
BspMI BsrDI BsrFI BstAPI BtgZI BtsI EaeI EarI
Eco57MI EcoRI Hin4I HindIII MscI NspI PasI PsiI
SexAI SfcI SmlI SphI SspI TatI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BfrBI BglI BglII Bme1580I BmgBI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseYI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI
BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI MmeI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI StyI SwaI TaqII TstI Tth111I
XcmI XhoI XmaI ZraI