Restriction map for
pF1KB7867
(Linear) (Six-base) MAPSORT of: KIBB7867.seq Check: 698 from: 1 to: 1680
KIBB7867.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 234 559 1318 1680
Size: 234 325 759 362
Fragments arranged by size:
759 362 325 234
AatII G_ACGT'C
Cuts at: 0 1634 1680
Size: 1634 46
AfeI AGC'GCT
Cuts at: 0 1234 1680
Size: 1234 446
AflIII A'CryG_T
Cuts at: 0 119 1680
Size: 119 1561
AlwNI CAG_nnn'CTG
Cuts at: 0 1077 1333 1680
Size: 1077 256 347
Fragments arranged by size:
1077 347 256
ApaI G_GGCC'C
Cuts at: 0 685 1680
Size: 685 995
AvaI C'yCGr_G
Cuts at: 0 1378 1483 1680
Size: 1378 105 197
Fragments arranged by size:
1378 197 105
BamHI G'GATC_C
Cuts at: 0 59 1680
Size: 59 1621
BanI G'GyrC_C
Cuts at: 0 246 537 966 1527 1680
Size: 246 291 429 561 153
Fragments arranged by size:
561 429 291 246 153
BanII G_rGCy'C
Cuts at: 0 365 685 1268 1680
Size: 365 320 583 412
Fragments arranged by size:
583 412 365 320
BbeI G_GCGC'C
Cuts at: 0 541 970 1680
Size: 541 429 710
Fragments arranged by size:
710 541 429
BbsI GAAGACnn'nnnn_
Cuts at: 0 21 1680
Size: 21 1659
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 771 805 1087 1121 1680
Size: 771 34 282 34 559
Fragments arranged by size:
771 559 282 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 649 1319 1680
Size: 649 670 361
Fragments arranged by size:
670 649 361
BlpI GC'TnA_GC
Cuts at: 0 1334 1588 1680
Size: 1334 254 92
Fragments arranged by size:
1334 254 92
Bme1580I G_kGCm'C
Cuts at: 0 685 1680
Size: 685 995
BmgBI CAC'GTC
Cuts at: 0 27 1680
Size: 27 1653
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 885 1680
Size: 885 795
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 644 1456 1538 1680
Size: 271 373 812 82 142
Fragments arranged by size:
812 373 271 142 82
BsaI GGTCTCn'nnnn_
Cuts at: 0 1240 1680
Size: 1240 440
BsaAI yAC'GTr
Cuts at: 0 122 1224 1671 1680
Size: 122 1102 447 9
Fragments arranged by size:
1102 447 122 9
BsaHI Gr'CG_yC
Cuts at: 0 538 967 1495 1631 1680
Size: 538 429 528 136 49
Fragments arranged by size:
538 528 429 136 49
BsaWI w'CCGG_w
Cuts at: 0 129 1680
Size: 129 1551
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 979 1009 1680
Size: 979 30 671
Fragments arranged by size:
979 671 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 285 964 993 1000 1003 1680
Size: 148 137 679 29 7 3 677
Fragments arranged by size:
679 677 148 137 29 7 3
BseYI C'CCAG_C
Cuts at: 0 541 874 1326 1462 1680
Size: 541 333 452 136 218
Fragments arranged by size:
541 452 333 218 136
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 1680
Size: 262 1418
BsiEI CG_ry'CG
Cuts at: 0 1346 1680
Size: 1346 334
BsiHKAI G_wGCw'C
Cuts at: 0 26 365 660 1680
Size: 26 339 295 1020
Fragments arranged by size:
1020 339 295 26
Bsp1286I G_dGCh'C
Cuts at: 0 26 365 660 685 1268 1680
Size: 26 339 295 25 583 412
Fragments arranged by size:
583 412 339 295 26 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 234 518 559 1109 1318 1358 1584 1680
Size: 234 284 41 550 209 40 226 96
Fragments arranged by size:
550 284 234 226 209 96 41 40
BsrBI CCG'CTC
Cuts at: 0 408 440 1680
Size: 408 32 1240
Fragments arranged by size:
1240 408 32
BsrFI r'CCGG_y
Cuts at: 0 280 643 702 1027 1491 1680
Size: 280 363 59 325 464 189
Fragments arranged by size:
464 363 325 280 189 59
BssHII G'CGCG_C
Cuts at: 0 329 1288 1680
Size: 329 959 392
Fragments arranged by size:
959 392 329
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1077 1235 1680
Size: 1077 158 445
Fragments arranged by size:
1077 445 158
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1314 1680
Size: 1314 366
BstYI r'GATC_y
Cuts at: 0 59 1680
Size: 59 1621
BtgI C'CryG_G
Cuts at: 0 1251 1680
Size: 1251 429
DraI TTT'AAA
Cuts at: 0 930 1680
Size: 930 750
DrdI GACnn_nn'nnGTC
Cuts at: 0 1494 1680
Size: 1494 186
EaeI y'GGCC_r
Cuts at: 0 525 641 1343 1680
Size: 525 116 702 337
Fragments arranged by size:
702 525 337 116
EagI C'GGCC_G
Cuts at: 0 1343 1680
Size: 1343 337
EarI CTCTTCn'nnn_
Cuts at: 0 205 1680
Size: 205 1475
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1558 1680
Size: 1558 122
EcoICRI GAG'CTC
Cuts at: 0 363 1680
Size: 363 1317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 644 1456 1538 1680
Size: 271 373 812 82 142
Fragments arranged by size:
812 373 271 142 82
EcoO109I rG'GnC_Cy
Cuts at: 0 681 682 1113 1680
Size: 681 1 431 567
Fragments arranged by size:
681 567 431 1
EcoRV GAT'ATC
Cuts at: 0 205 1680
Size: 205 1475
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1077 1623 1680
Size: 1077 546 57
Fragments arranged by size:
1077 546 57
FspI TGC'GCA
Cuts at: 0 1228 1680
Size: 1228 452
HaeII r_GCGC'y
Cuts at: 0 541 906 970 1236 1341 1680
Size: 541 365 64 266 105 339
Fragments arranged by size:
541 365 339 266 105 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 189 221 979 1011 1015 1047 1680
Size: 189 32 758 32 4 32 633
Fragments arranged by size:
758 633 189 32 32 32 4
KasI G'GCGC_C
Cuts at: 0 537 966 1680
Size: 537 429 714
Fragments arranged by size:
714 537 429
MspA1I CmG'CkG
Cuts at: 0 13 195 1333 1525 1680
Size: 13 182 1138 192 155
Fragments arranged by size:
1138 192 182 155 13
NaeI GCC'GGC
Cuts at: 0 704 1029 1680
Size: 704 325 651
Fragments arranged by size:
704 651 325
NarI GG'CG_CC
Cuts at: 0 538 967 1680
Size: 538 429 713
Fragments arranged by size:
713 538 429
NgoMIV G'CCGG_C
Cuts at: 0 702 1027 1680
Size: 702 325 653
Fragments arranged by size:
702 653 325
NspI r_CATG'y
Cuts at: 0 822 1680
Size: 822 858
PflMI CCAn_nnn'nTGG
Cuts at: 0 16 1680
Size: 16 1664
PfoI T'CCnGG_A
Cuts at: 0 143 287 1680
Size: 143 144 1393
Fragments arranged by size:
1393 144 143
PmlI CAC'GTG
Cuts at: 0 122 1224 1680
Size: 122 1102 456
Fragments arranged by size:
1102 456 122
PpuMI rG'GwC_Cy
Cuts at: 0 1113 1680
Size: 1113 567
PspOMI G'GGCC_C
Cuts at: 0 681 1680
Size: 681 999
PstI C_TGCA'G
Cuts at: 0 515 1082 1581 1680
Size: 515 567 499 99
Fragments arranged by size:
567 515 499 99
PvuII CAG'CTG
Cuts at: 0 13 195 1680
Size: 13 182 1485
Fragments arranged by size:
1485 182 13
SacI G_AGCT'C
Cuts at: 0 365 1680
Size: 365 1315
SanDI GG'GwC_CC
Cuts at: 0 1113 1680
Size: 1113 567
ScaI AGT'ACT
Cuts at: 0 448 1680
Size: 448 1232
SfcI C'TryA_G
Cuts at: 0 511 1078 1577 1680
Size: 511 567 499 103
Fragments arranged by size:
567 511 499 103
SfoI GGC'GCC
Cuts at: 0 539 968 1680
Size: 539 429 712
Fragments arranged by size:
712 539 429
SgrAI Cr'CCGG_yG
Cuts at: 0 1491 1680
Size: 1491 189
SphI G_CATG'C
Cuts at: 0 822 1680
Size: 822 858
StuI AGG'CCT
Cuts at: 0 1060 1680
Size: 1060 620
StyI C'CwwG_G
Cuts at: 0 1052 1680
Size: 1052 628
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1545 1680
Size: 1545 135
TatI w'GTAC_w
Cuts at: 0 446 1680
Size: 446 1234
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 770 802 1680
Size: 770 32 878
Fragments arranged by size:
878 770 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 234 321 618 1680
Size: 234 87 297 1062
Fragments arranged by size:
1062 297 234 87
ZraI GAC'GTC
Cuts at: 0 1632 1680
Size: 1632 48
Enzymes that do cut:
AarI AatII AfeI AflIII AlwNI ApaI AvaI BamHI
BanI BanII BbeI BbsI BcgI BglI BlpI Bme1580I
BmgBI BplI BpmI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BssHII BstAPI BstXI BstYI BtgI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV
FalI FspI HaeII Hin4I KasI MspA1I NaeI NarI
NgoMIV NspI PflMI PfoI PmlI PpuMI PspOMI PstI
PvuII SacI SanDI ScaI SfcI SfoI SgrAI SphI
StuI StyI TaqII TatI TstI XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AflII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbvCI BciVI BclI BfrBI BglII BmrI BmtI
Bpu10I BpuEI BsaBI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraIII EcoNI EcoRI FseI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PciI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SapI SbfI SexAI SfiI
SgfI SmaI SmlI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI XmnI