Restriction map for
pF1KB7864
(Linear) (Six-base) MAPSORT of: KIBB7864.seq Check: 8191 from: 1 to: 1893
KIBB7864.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 936 1739 1835 1893
Size: 936 803 96 58
Fragments arranged by size:
936 803 96 58
AccI GT'mk_AC
Cuts at: 0 791 1033 1893
Size: 791 242 860
Fragments arranged by size:
860 791 242
AflIII A'CryG_T
Cuts at: 0 851 1893
Size: 851 1042
AleI CACnn'nnGTG
Cuts at: 0 781 1893
Size: 781 1112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1801 1893
Size: 1801 92
AlwNI CAG_nnn'CTG
Cuts at: 0 87 639 759 1117 1401 1639 1731 1893
Size: 87 552 120 358 284 238 92 162
Fragments arranged by size:
552 358 284 238 162 120 92 87
ApaI G_GGCC'C
Cuts at: 0 104 622 866 1126 1893
Size: 104 518 244 260 767
Fragments arranged by size:
767 518 260 244 104
AvaI C'yCGr_G
Cuts at: 0 200 882 1519 1893
Size: 200 682 637 374
Fragments arranged by size:
682 637 374 200
BanI G'GyrC_C
Cuts at: 0 1288 1893
Size: 1288 605
BanII G_rGCy'C
Cuts at: 0 35 104 208 566 622 766 866 1073
Size: 35 69 104 358 56 144 100 207
Cuts at: 1073 1126 1495 1801 1893
Size: 53 369 306 92
Fragments arranged by size:
369 358 306 207 144 104 100 92
69 56 53 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 242 469 1617 1893
Size: 242 227 1148 276
Fragments arranged by size:
1148 276 242 227
BbvCI CC'TCA_GC
Cuts at: 0 274 1893
Size: 274 1619
BfrBI ATG'CAT
Cuts at: 0 722 1893
Size: 722 1171
BglI GCCn_nnn'nGGC
Cuts at: 0 186 1477 1893
Size: 186 1291 416
Fragments arranged by size:
1291 416 186
BlpI GC'TnA_GC
Cuts at: 0 61 173 1481 1750 1893
Size: 61 112 1308 269 143
Fragments arranged by size:
1308 269 143 112 61
Bme1580I G_kGCm'C
Cuts at: 0 104 454 622 866 1126 1893
Size: 104 350 168 244 260 767
Fragments arranged by size:
767 350 260 244 168 104
BmgBI CAC'GTC
Cuts at: 0 1698 1893
Size: 1698 195
BmrI ACTGGGnnnn_n'
Cuts at: 0 97 935 1524 1893
Size: 97 838 589 369
Fragments arranged by size:
838 589 369 97
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1275 1893
Size: 1275 618
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 70 137 286 1087 1632 1758 1791
Size: 64 6 67 149 801 545 126 33
Cuts at: 1791 1893
Size: 102
Fragments arranged by size:
801 545 149 126 102 67 64 33
6
Bpu10I CC'TnA_GC
Cuts at: 0 274 1588 1893
Size: 274 1314 305
Fragments arranged by size:
1314 305 274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 987 1893
Size: 987 906
BsaI GGTCTCn'nnnn_
Cuts at: 0 307 1113 1849 1893
Size: 307 806 736 44
Fragments arranged by size:
806 736 307 44
BsaAI yAC'GTr
Cuts at: 0 854 1893
Size: 854 1039
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 319 349 591 621 1893
Size: 319 30 242 30 1272
Fragments arranged by size:
1272 319 242 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 298 694 715 1893
Size: 298 396 21 1178
Fragments arranged by size:
1178 396 298 21
BseYI C'CCAG_C
Cuts at: 0 189 621 629 759 1072 1117 1394 1702
Size: 189 432 8 130 313 45 277 308
Cuts at: 1702 1893
Size: 191
Fragments arranged by size:
432 313 308 277 191 189 130 45
8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1141 1387 1459 1893
Size: 1141 246 72 434
Fragments arranged by size:
1141 434 246 72
BsiEI CG_ry'CG
Cuts at: 0 159 1893
Size: 159 1734
BsiHKAI G_wGCw'C
Cuts at: 0 35 1801 1893
Size: 35 1766 92
Fragments arranged by size:
1766 92 35
BsmI GAATG_Cn'
Cuts at: 0 724 1893
Size: 724 1169
BsmBI CGTCTCn'nnnn_
Cuts at: 0 211 682 1893
Size: 211 471 1211
Fragments arranged by size:
1211 471 211
Bsp1286I G_dGCh'C
Cuts at: 0 35 104 208 454 566 622 766 866
Size: 35 69 104 246 112 56 144 100
Cuts at: 866 1073 1126 1495 1801 1893
Size: 207 53 369 306 92
Fragments arranged by size:
369 306 246 207 144 112 104 100
92 69 56 53 35
BspHI T'CATG_A
Cuts at: 0 1232 1893
Size: 1232 661
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 374 897 936 1611 1739 1835 1893
Size: 374 523 39 675 128 96 58
Fragments arranged by size:
675 523 374 128 96 58 39
BsrBI CCG'CTC
Cuts at: 0 901 1425 1893
Size: 901 524 468
Fragments arranged by size:
901 524 468
BsrDI GCAATG_nn'
Cuts at: 0 1818 1893
Size: 1818 75
BsrFI r'CCGG_y
Cuts at: 0 809 1338 1671 1717 1737 1745 1893
Size: 809 529 333 46 20 8 148
Fragments arranged by size:
809 529 333 148 46 20 8
BsrGI T'GTAC_A
Cuts at: 0 485 1893
Size: 485 1408
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1563 1893
Size: 1563 330
BstXI CCAn_nnnn'nTGG
Cuts at: 0 189 1065 1782 1875 1893
Size: 189 876 717 93 18
Fragments arranged by size:
876 717 189 93 18
BstYI r'GATC_y
Cuts at: 0 635 1893
Size: 635 1258
Bsu36I CC'TnA_GG
Cuts at: 0 281 1893
Size: 281 1612
BtgI C'CryG_G
Cuts at: 0 305 334 845 951 1058 1475 1893
Size: 305 29 511 106 107 417 418
Fragments arranged by size:
511 418 417 305 107 106 29
BtsI GCAGTG_nn'
Cuts at: 0 73 1153 1893
Size: 73 1080 740
Fragments arranged by size:
1080 740 73
DraIII CAC_nnn'GTG
Cuts at: 0 91 957 1050 1333 1893
Size: 91 866 93 283 560
Fragments arranged by size:
866 560 283 93 91
EaeI y'GGCC_r
Cuts at: 0 842 1469 1598 1815 1893
Size: 842 627 129 217 78
Fragments arranged by size:
842 627 217 129 78
EarI CTCTTCn'nnn_
Cuts at: 0 554 1887 1893
Size: 554 1333 6
Fragments arranged by size:
1333 554 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 612 730 1893
Size: 612 118 1163
Fragments arranged by size:
1163 612 118
EcoICRI GAG'CTC
Cuts at: 0 33 1799 1893
Size: 33 1766 94
Fragments arranged by size:
1766 94 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 70 137 286 1087 1632 1758 1791
Size: 64 6 67 149 801 545 126 33
Cuts at: 1791 1893
Size: 102
Fragments arranged by size:
801 545 149 126 102 67 64 33
6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 133 322 445 643 654 1893
Size: 133 189 123 198 11 1239
Fragments arranged by size:
1239 198 189 133 123 11
EcoO109I rG'GnC_Cy
Cuts at: 0 100 101 124 330 618 863 886 1122
Size: 100 1 23 206 288 245 23 236
Cuts at: 1122 1123 1267 1710 1893
Size: 1 144 443 183
Fragments arranged by size:
443 288 245 236 206 183 144 100
23 23 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1641 1893
Size: 1641 252
HaeII r_GCGC'y
Cuts at: 0 1595 1757 1893
Size: 1595 162 136
Fragments arranged by size:
1595 162 136
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1159 1191 1893
Size: 1159 32 702
Fragments arranged by size:
1159 702 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 791 1862 1893
Size: 791 1071 31
Fragments arranged by size:
1071 791 31
MscI TGG'CCA
Cuts at: 0 844 1471 1600 1817 1893
Size: 844 627 129 217 76
Fragments arranged by size:
844 627 217 129 76
MslI CAynn'nnrTG
Cuts at: 0 457 781 1873 1893
Size: 457 324 1092 20
Fragments arranged by size:
1092 457 324 20
MspA1I CmG'CkG
Cuts at: 0 21 390 860 1010 1117 1401 1485 1893
Size: 21 369 470 150 107 284 84 408
Fragments arranged by size:
470 408 369 284 150 107 84 21
NaeI GCC'GGC
Cuts at: 0 1673 1719 1739 1893
Size: 1673 46 20 154
Fragments arranged by size:
1673 154 46 20
NcoI C'CATG_G
Cuts at: 0 845 1475 1893
Size: 845 630 418
Fragments arranged by size:
845 630 418
NgoMIV G'CCGG_C
Cuts at: 0 1671 1717 1737 1893
Size: 1671 46 20 156
Fragments arranged by size:
1671 156 46 20
NsiI A_TGCA'T
Cuts at: 0 724 1893
Size: 724 1169
PasI CC'CwG_GG
Cuts at: 0 871 1263 1893
Size: 871 392 630
Fragments arranged by size:
871 630 392
PflMI CCAn_nnn'nTGG
Cuts at: 0 87 1893
Size: 87 1806
PpuMI rG'GwC_Cy
Cuts at: 0 330 886 1267 1710 1893
Size: 330 556 381 443 183
Fragments arranged by size:
556 443 381 330 183
PspOMI G'GGCC_C
Cuts at: 0 100 618 862 1122 1893
Size: 100 518 244 260 771
Fragments arranged by size:
771 518 260 244 100
PstI C_TGCA'G
Cuts at: 0 26 326 371 993 1490 1736 1893
Size: 26 300 45 622 497 246 157
Fragments arranged by size:
622 497 300 246 157 45 26
PvuII CAG'CTG
Cuts at: 0 21 1117 1485 1893
Size: 21 1096 368 408
Fragments arranged by size:
1096 408 368 21
SacI G_AGCT'C
Cuts at: 0 35 1801 1893
Size: 35 1766 92
Fragments arranged by size:
1766 92 35
SapI GCTCTTCn'nnn_
Cuts at: 0 554 1893
Size: 554 1339
SexAI A'CCwGG_T
Cuts at: 0 490 1893
Size: 490 1403
SfcI C'TryA_G
Cuts at: 0 22 322 367 989 1486 1506 1732 1893
Size: 22 300 45 622 497 20 226 161
Fragments arranged by size:
622 497 300 226 161 45 22 20
SmaI CCC'GGG
Cuts at: 0 884 1893
Size: 884 1009
SmlI C'TyrA_G
Cuts at: 0 1002 1893
Size: 1002 891
StuI AGG'CCT
Cuts at: 0 649 1184 1645 1893
Size: 649 535 461 248
Fragments arranged by size:
649 535 461 248
StyI C'CwwG_G
Cuts at: 0 845 1475 1893
Size: 845 630 418
Fragments arranged by size:
845 630 418
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 79 264 547 1552 1885 1893
Size: 79 185 283 1005 333 8
Fragments arranged by size:
1005 333 283 185 79 8
TatI w'GTAC_w
Cuts at: 0 485 1090 1532 1893
Size: 485 605 442 361
Fragments arranged by size:
605 485 442 361
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 319 351 1036 1068 1893
Size: 319 32 685 32 825
Fragments arranged by size:
825 685 319 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1029 1893
Size: 1029 864
XmaI C'CCGG_G
Cuts at: 0 882 1893
Size: 882 1011
XmnI GAAnn'nnTTC
Cuts at: 0 607 1462 1893
Size: 607 855 431
Fragments arranged by size:
855 607 431
Enzymes that do cut:
AarI AccI AflIII AleI AlfI AlwNI ApaI AvaI
BanI BanII BbsI BbvCI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI
BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI
PasI PflMI PpuMI PspOMI PstI PvuII SacI SapI
SexAI SfcI SmaI SmlI StuI StyI TaqII TatI
TstI Tth111I XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BciVI BclI BglII BmtI BsaBI
BsaHI BsaWI BsiWI BspEI BssHII BssSI BstBI BstEII
BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NspI PacI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XcmI XhoI ZraI