Restriction map for
pF1KB7861
(Linear) (Six-base) MAPSORT of: KIBB7861.seq Check: 426 from: 1 to: 1662
KIBB7861.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1102 1662
Size: 1102 560
Acc65I G'GTAC_C
Cuts at: 0 111 1662
Size: 111 1551
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 477 645 933 1101 1353 1437 1521
Size: 101 376 168 288 168 252 84 84
Cuts at: 1521 1662
Size: 141
Fragments arranged by size:
376 288 252 168 168 141 101 84
84
AfeI AGC'GCT
Cuts at: 0 341 1030 1198 1662
Size: 341 689 168 464
Fragments arranged by size:
689 464 341 168
AflIII A'CryG_T
Cuts at: 0 1175 1662
Size: 1175 487
AgeI A'CCGG_T
Cuts at: 0 923 1662
Size: 923 739
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 350 1662
Size: 350 1312
ApaI G_GGCC'C
Cuts at: 0 513 1589 1662
Size: 513 1076 73
Fragments arranged by size:
1076 513 73
ApaLI G'TGCA_C
Cuts at: 0 588 1662
Size: 588 1074
AvaI C'yCGr_G
Cuts at: 0 302 401 540 1351 1662
Size: 302 99 139 811 311
Fragments arranged by size:
811 311 302 139 99
BamHI G'GATC_C
Cuts at: 0 287 1662
Size: 287 1375
BanI G'GyrC_C
Cuts at: 0 111 205 219 862 1234 1246 1662
Size: 111 94 14 643 372 12 416
Fragments arranged by size:
643 416 372 111 94 14 12
BanII G_rGCy'C
Cuts at: 0 158 201 273 513 547 733 818 1297
Size: 158 43 72 240 34 186 85 479
Cuts at: 1297 1589 1635 1662
Size: 292 46 27
Fragments arranged by size:
479 292 240 186 158 85 72 46
43 34 27
BbeI G_GCGC'C
Cuts at: 0 866 1250 1662
Size: 866 384 412
Fragments arranged by size:
866 412 384
BlpI GC'TnA_GC
Cuts at: 0 764 1662
Size: 764 898
Bme1580I G_kGCm'C
Cuts at: 0 222 513 592 1237 1267 1351 1589 1662
Size: 222 291 79 645 30 84 238 73
Fragments arranged by size:
645 291 238 222 84 79 73 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 490 797 1337 1662
Size: 490 307 540 325
Fragments arranged by size:
540 490 325 307
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 186 1662
Size: 186 1476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 531 1662
Size: 531 1131
Bpu10I CC'TnA_GC
Cuts at: 0 732 1662
Size: 732 930
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 500 1662
Size: 185 315 1162
Fragments arranged by size:
1162 315 185
BsaI GGTCTCn'nnnn_
Cuts at: 0 314 1662
Size: 314 1348
BsaAI yAC'GTr
Cuts at: 0 1176 1662
Size: 1176 486
BsaHI Gr'CG_yC
Cuts at: 0 692 863 1247 1662
Size: 692 171 384 415
Fragments arranged by size:
692 415 384 171
BsaWI w'CCGG_w
Cuts at: 0 923 1159 1662
Size: 923 236 503
Fragments arranged by size:
923 503 236
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 334 421 865 868 895 1662
Size: 334 87 444 3 27 767
Fragments arranged by size:
767 444 334 87 27 3
BseYI C'CCAG_C
Cuts at: 0 99 215 1611 1662
Size: 99 116 1396 51
Fragments arranged by size:
1396 116 99 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 640 996 1540 1662
Size: 124 516 356 544 122
Fragments arranged by size:
544 516 356 124 122
BsiEI CG_ry'CG
Cuts at: 0 726 1038 1185 1662
Size: 726 312 147 477
Fragments arranged by size:
726 477 312 147
BsiHKAI G_wGCw'C
Cuts at: 0 592 733 1297 1662
Size: 592 141 564 365
Fragments arranged by size:
592 564 365 141
Bsp1286I G_dGCh'C
Cuts at: 0 158 201 222 273 513 547 592 733
Size: 158 43 21 51 240 34 45 141
Cuts at: 733 818 1237 1267 1297 1351 1589 1635 1662
Size: 85 419 30 30 54 238 46 27
Fragments arranged by size:
419 240 238 158 141 85 54 51
46 45 43 34 30 30 27 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1102 1562 1662
Size: 1102 460 100
Fragments arranged by size:
1102 460 100
BsrBI CCG'CTC
Cuts at: 0 1211 1654 1662
Size: 1211 443 8
Fragments arranged by size:
1211 443 8
BsrFI r'CCGG_y
Cuts at: 0 323 551 738 759 923 977 1541 1559
Size: 323 228 187 21 164 54 564 18
Cuts at: 1559 1662
Size: 103
Fragments arranged by size:
564 323 228 187 164 103 54 21
18
BssHII G'CGCG_C
Cuts at: 0 704 1662
Size: 704 958
BssSI C'ACGA_G
Cuts at: 0 1564 1662
Size: 1564 98
BstAPI GCAn_nnn'nTGC
Cuts at: 0 618 1019 1662
Size: 618 401 643
Fragments arranged by size:
643 618 401
BstEII G'GTnAC_C
Cuts at: 0 1221 1662
Size: 1221 441
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1596 1662
Size: 1596 66
BstYI r'GATC_y
Cuts at: 0 287 1571 1662
Size: 287 1284 91
Fragments arranged by size:
1284 287 91
BtgI C'CryG_G
Cuts at: 0 632 809 1494 1662
Size: 632 177 685 168
Fragments arranged by size:
685 632 177 168
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 167 1374 1662
Size: 167 1207 288
Fragments arranged by size:
1207 288 167
BtsI GCAGTG_nn'
Cuts at: 0 136 347 706 940 1428 1662
Size: 136 211 359 234 488 234
Fragments arranged by size:
488 359 234 234 211 136
DraIII CAC_nnn'GTG
Cuts at: 0 969 1662
Size: 969 693
EaeI y'GGCC_r
Cuts at: 0 723 740 1035 1598 1637 1662
Size: 723 17 295 563 39 25
Fragments arranged by size:
723 563 295 39 25 17
EagI C'GGCC_G
Cuts at: 0 723 1035 1662
Size: 723 312 627
Fragments arranged by size:
723 627 312
EarI CTCTTCn'nnn_
Cuts at: 0 155 259 910 1105 1662
Size: 155 104 651 195 557
Fragments arranged by size:
651 557 195 155 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 29 281 782 1662
Size: 29 252 501 880
Fragments arranged by size:
880 501 252 29
EcoICRI GAG'CTC
Cuts at: 0 731 1295 1662
Size: 731 564 367
Fragments arranged by size:
731 564 367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 477 531 645 933 1101 1353 1437
Size: 101 376 54 114 288 168 252 84
Cuts at: 1437 1521 1662
Size: 84 141
Fragments arranged by size:
376 288 252 168 141 114 101 84
84 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 495 1662
Size: 495 1167
EcoO109I rG'GnC_Cy
Cuts at: 0 510 885 1593 1662
Size: 510 375 708 69
Fragments arranged by size:
708 510 375 69
FseI GG_CCGG'CC
Cuts at: 0 742 1662
Size: 742 920
HaeII r_GCGC'y
Cuts at: 0 343 866 1032 1200 1250 1303 1548 1662
Size: 343 523 166 168 50 53 245 114
Fragments arranged by size:
523 343 245 168 166 114 53 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 1662
Size: 166 32 1464
Fragments arranged by size:
1464 166 32
KasI G'GCGC_C
Cuts at: 0 862 1246 1662
Size: 862 384 416
Fragments arranged by size:
862 416 384
KpnI G_GTAC'C
Cuts at: 0 115 1662
Size: 115 1547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1406 1574 1662
Size: 1406 168 88
Fragments arranged by size:
1406 168 88
MscI TGG'CCA
Cuts at: 0 1600 1639 1662
Size: 1600 39 23
Fragments arranged by size:
1600 39 23
MslI CAynn'nnrTG
Cuts at: 0 148 1606 1662
Size: 148 1458 56
Fragments arranged by size:
1458 148 56
MspA1I CmG'CkG
Cuts at: 0 953 1187 1662
Size: 953 234 475
Fragments arranged by size:
953 475 234
NaeI GCC'GGC
Cuts at: 0 553 740 761 1543 1662
Size: 553 187 21 782 119
Fragments arranged by size:
782 553 187 119 21
NarI GG'CG_CC
Cuts at: 0 863 1247 1662
Size: 863 384 415
Fragments arranged by size:
863 415 384
NgoMIV G'CCGG_C
Cuts at: 0 551 738 759 1541 1662
Size: 551 187 21 782 121
Fragments arranged by size:
782 551 187 121 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 67 1662
Size: 67 1595
PfoI T'CCnGG_A
Cuts at: 0 23 833 1662
Size: 23 810 829
Fragments arranged by size:
829 810 23
PshAI GACnn'nnGTC
Cuts at: 0 803 1662
Size: 803 859
PspOMI G'GGCC_C
Cuts at: 0 509 1585 1662
Size: 509 1076 77
Fragments arranged by size:
1076 509 77
PstI C_TGCA'G
Cuts at: 0 9 339 461 1662
Size: 9 330 122 1201
Fragments arranged by size:
1201 330 122 9
PvuII CAG'CTG
Cuts at: 0 953 1662
Size: 953 709
SacI G_AGCT'C
Cuts at: 0 733 1297 1662
Size: 733 564 365
Fragments arranged by size:
733 564 365
SapI GCTCTTCn'nnn_
Cuts at: 0 155 910 1105 1662
Size: 155 755 195 557
Fragments arranged by size:
755 557 195 155
SexAI A'CCwGG_T
Cuts at: 0 965 1662
Size: 965 697
SfcI C'TryA_G
Cuts at: 0 5 335 444 457 1662
Size: 5 330 109 13 1205
Fragments arranged by size:
1205 330 109 13 5
SfoI GGC'GCC
Cuts at: 0 864 1248 1662
Size: 864 384 414
Fragments arranged by size:
864 414 384
SgrAI Cr'CCGG_yG
Cuts at: 0 977 1662
Size: 977 685
SmaI CCC'GGG
Cuts at: 0 403 1662
Size: 403 1259
SmlI C'TyrA_G
Cuts at: 0 200 515 1662
Size: 200 315 1147
Fragments arranged by size:
1147 315 200
StuI AGG'CCT
Cuts at: 0 501 538 1662
Size: 501 37 1124
Fragments arranged by size:
1124 501 37
StyI C'CwwG_G
Cuts at: 0 642 1589 1662
Size: 642 947 73
Fragments arranged by size:
947 642 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 46 1240 1252 1324 1336 1504 1662
Size: 46 1194 12 72 12 168 158
Fragments arranged by size:
1194 168 158 72 46 12 12
Tth111I GACn'n_nGTC
Cuts at: 0 1219 1662
Size: 1219 443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1608 1662
Size: 1608 54
XmaI C'CCGG_G
Cuts at: 0 401 1662
Size: 401 1261
XmnI GAAnn'nnTTC
Cuts at: 0 658 1030 1114 1662
Size: 658 372 84 548
Fragments arranged by size:
658 548 372 84
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AgeI AlfI ApaI
ApaLI AvaI BamHI BanI BanII BbeI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
PflMI PfoI PshAI PspOMI PstI PvuII SacI SapI
SexAI SfcI SfoI SgrAI SmaI SmlI StuI StyI
TaqII Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AhdI AleI AloI AlwNI
ApoI AscI AseI AsiSI AvrII BaeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BmgBI BmtI
BsaBI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI
EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI
MfeI MluI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PpuMI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SfiI SgfI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI XbaI XhoI ZraI