Restriction map for
pF1KB7855
(Linear) (Six-base) MAPSORT of: KIBB7855.seq Check: 9373 from: 1 to: 1653
KIBB7855.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1278 1653
Size: 1278 375
AccI GT'mk_AC
Cuts at: 0 1406 1653
Size: 1406 247
AleI CACnn'nnGTG
Cuts at: 0 1096 1653
Size: 1096 557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1012 1653
Size: 1012 641
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 1653
Size: 27 1626
AlwNI CAG_nnn'CTG
Cuts at: 0 345 864 1018 1177 1225 1428 1431 1543
Size: 345 519 154 159 48 203 3 112
Cuts at: 1543 1653
Size: 110
Fragments arranged by size:
519 345 203 159 154 112 110 48
3
ApaI G_GGCC'C
Cuts at: 0 1521 1560 1653
Size: 1521 39 93
Fragments arranged by size:
1521 93 39
ApaLI G'TGCA_C
Cuts at: 0 730 1653
Size: 730 923
ApoI r'AATT_y
Cuts at: 0 853 1653
Size: 853 800
AvaI C'yCGr_G
Cuts at: 0 704 1560 1653
Size: 704 856 93
Fragments arranged by size:
856 704 93
BanII G_rGCy'C
Cuts at: 0 311 581 839 848 1472 1521 1560 1568
Size: 311 270 258 9 624 49 39 8
Cuts at: 1568 1653
Size: 85
Fragments arranged by size:
624 311 270 258 85 49 39 9
8
BbsI GAAGACnn'nnnn_
Cuts at: 0 1349 1601 1653
Size: 1349 252 52
Fragments arranged by size:
1349 252 52
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 445 479 600 634 1653
Size: 445 34 121 34 1019
Fragments arranged by size:
1019 445 121 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 290 1653
Size: 290 1363
BglII A'GATC_T
Cuts at: 0 584 632 1653
Size: 584 48 1021
Fragments arranged by size:
1021 584 48
BlpI GC'TnA_GC
Cuts at: 0 305 1011 1653
Size: 305 706 642
Fragments arranged by size:
706 642 305
Bme1580I G_kGCm'C
Cuts at: 0 564 734 1521 1560 1653
Size: 564 170 787 39 93
Fragments arranged by size:
787 564 170 93 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 1225 1561 1653
Size: 1225 336 92
Fragments arranged by size:
1225 336 92
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 138 1653
Size: 138 1515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 1154 1172 1653
Size: 397 757 18 481
Fragments arranged by size:
757 481 397 18
Bpu10I CC'TnA_GC
Cuts at: 0 1063 1653
Size: 1063 590
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 1653
Size: 565 1088
BsaI GGTCTCn'nnnn_
Cuts at: 0 913 1653
Size: 913 740
BsaWI w'CCGG_w
Cuts at: 0 755 887 1653
Size: 755 132 766
Fragments arranged by size:
766 755 132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1588 1618 1653
Size: 1588 30 35
Fragments arranged by size:
1588 35 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 162 233 910 1241 1593 1653
Size: 162 71 677 331 352 60
Fragments arranged by size:
677 352 331 162 71 60
BseYI C'CCAG_C
Cuts at: 0 695 778 1037 1218 1291 1377 1536 1653
Size: 695 83 259 181 73 86 159 117
Fragments arranged by size:
695 259 181 159 117 86 83 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 1653
Size: 676 977
BsiEI CG_ry'CG
Cuts at: 0 831 882 1653
Size: 831 51 771
Fragments arranged by size:
831 771 51
BsiHKAI G_wGCw'C
Cuts at: 0 154 311 581 734 839 1069 1653
Size: 154 157 270 153 105 230 584
Fragments arranged by size:
584 270 230 157 154 153 105
BsmI GAATG_Cn'
Cuts at: 0 468 1653
Size: 468 1185
Bsp1286I G_dGCh'C
Cuts at: 0 154 311 564 581 734 839 848 1069
Size: 154 157 253 17 153 105 9 221
Cuts at: 1069 1472 1521 1560 1568 1653
Size: 403 49 39 8 85
Fragments arranged by size:
403 253 221 157 154 153 105 85
49 39 17 9 8
BspEI T'CCGG_A
Cuts at: 0 755 1653
Size: 755 898
BspHI T'CATG_A
Cuts at: 0 935 1653
Size: 935 718
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 872 1278 1653
Size: 872 406 375
Fragments arranged by size:
872 406 375
BsrDI GCAATG_nn'
Cuts at: 0 989 1607 1653
Size: 989 618 46
Fragments arranged by size:
989 618 46
BsrFI r'CCGG_y
Cuts at: 0 248 1653
Size: 248 1405
BssSI C'ACGA_G
Cuts at: 0 262 1653
Size: 262 1391
BstEII G'GTnAC_C
Cuts at: 0 228 1653
Size: 228 1425
BstXI CCAn_nnnn'nTGG
Cuts at: 0 49 1479 1653
Size: 49 1430 174
Fragments arranged by size:
1430 174 49
BstYI r'GATC_y
Cuts at: 0 584 632 1653
Size: 584 48 1021
Fragments arranged by size:
1021 584 48
Bsu36I CC'TnA_GG
Cuts at: 0 1253 1490 1653
Size: 1253 237 163
Fragments arranged by size:
1253 237 163
BtgI C'CryG_G
Cuts at: 0 125 848 1094 1193 1653
Size: 125 723 246 99 460
Fragments arranged by size:
723 460 246 125 99
BtsI GCAGTG_nn'
Cuts at: 0 569 1653
Size: 569 1084
EaeI y'GGCC_r
Cuts at: 0 743 1653
Size: 743 910
EarI CTCTTCn'nnn_
Cuts at: 0 431 937 1653
Size: 431 506 716
Fragments arranged by size:
716 506 431
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 508 1653
Size: 508 1145
EcoICRI GAG'CTC
Cuts at: 0 309 579 837 1653
Size: 309 270 258 816
Fragments arranged by size:
816 309 270 258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 1154 1172 1653
Size: 397 757 18 481
Fragments arranged by size:
757 481 397 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 379 1162 1653
Size: 379 783 491
Fragments arranged by size:
783 491 379
EcoO109I rG'GnC_Cy
Cuts at: 0 237 372 498 512 1215 1246 1314 1352
Size: 237 135 126 14 703 31 68 38
Cuts at: 1352 1517 1526 1556 1557 1653
Size: 165 9 30 1 96
Fragments arranged by size:
703 237 165 135 126 96 68 38
31 30 14 9 1
EcoRI G'AATT_C
Cuts at: 0 853 1653
Size: 853 800
FspI TGC'GCA
Cuts at: 0 217 1653
Size: 217 1436
FspAI rTGC'GCAy
Cuts at: 0 217 1653
Size: 217 1436
HaeII r_GCGC'y
Cuts at: 0 123 1653
Size: 123 1530
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1384 1416 1588 1620 1653
Size: 1384 32 172 32 33
Fragments arranged by size:
1384 172 33 32 32
HincII GTy'rAC
Cuts at: 0 1407 1653
Size: 1407 246
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 851 1653
Size: 851 802
MscI TGG'CCA
Cuts at: 0 745 1653
Size: 745 908
MslI CAynn'nnrTG
Cuts at: 0 673 994 1096 1447 1477 1653
Size: 673 321 102 351 30 176
Fragments arranged by size:
673 351 321 176 102 30
MspA1I CmG'CkG
Cuts at: 0 87 127 323 614 813 956 1326 1381
Size: 87 40 196 291 199 143 370 55
Cuts at: 1381 1428 1653
Size: 47 225
Fragments arranged by size:
370 291 225 199 196 143 87 55
47 40
NcoI C'CATG_G
Cuts at: 0 848 1094 1193 1653
Size: 848 246 99 460
Fragments arranged by size:
848 460 246 99
NdeI CA'TA_TG
Cuts at: 0 915 1653
Size: 915 738
NspI r_CATG'y
Cuts at: 0 96 1653
Size: 96 1557
PflMI CCAn_nnn'nTGG
Cuts at: 0 345 1653
Size: 345 1308
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 589 621 1653
Size: 589 32 1032
Fragments arranged by size:
1032 589 32
PpuMI rG'GwC_Cy
Cuts at: 0 237 372 498 1653
Size: 237 135 126 1155
Fragments arranged by size:
1155 237 135 126
PshAI GACnn'nnGTC
Cuts at: 0 881 1653
Size: 881 772
PspOMI G'GGCC_C
Cuts at: 0 1517 1556 1653
Size: 1517 39 97
Fragments arranged by size:
1517 97 39
PstI C_TGCA'G
Cuts at: 0 788 1325 1331 1653
Size: 788 537 6 322
Fragments arranged by size:
788 537 322 6
PvuI CG_AT'CG
Cuts at: 0 882 1653
Size: 882 771
PvuII CAG'CTG
Cuts at: 0 614 813 1326 1381 1428 1653
Size: 614 199 513 55 47 225
Fragments arranged by size:
614 513 225 199 55 47
RsrII CG'GwC_CG
Cuts at: 0 318 1653
Size: 318 1335
SacI G_AGCT'C
Cuts at: 0 311 581 839 1653
Size: 311 270 258 814
Fragments arranged by size:
814 311 270 258
SacII CC_GC'GG
Cuts at: 0 128 1653
Size: 128 1525
SalI G'TCGA_C
Cuts at: 0 1405 1653
Size: 1405 248
SanDI GG'GwC_CC
Cuts at: 0 498 1653
Size: 498 1155
SapI GCTCTTCn'nnn_
Cuts at: 0 431 1653
Size: 431 1222
SbfI CC_TGCA'GG
Cuts at: 0 788 1653
Size: 788 865
SfcI C'TryA_G
Cuts at: 0 431 784 1321 1327 1653
Size: 431 353 537 6 326
Fragments arranged by size:
537 431 353 326 6
SmaI CCC'GGG
Cuts at: 0 1562 1653
Size: 1562 91
SmlI C'TyrA_G
Cuts at: 0 580 1653
Size: 580 1073
SphI G_CATG'C
Cuts at: 0 96 1653
Size: 96 1557
StuI AGG'CCT
Cuts at: 0 46 1126 1653
Size: 46 1080 527
Fragments arranged by size:
1080 527 46
StyI C'CwwG_G
Cuts at: 0 233 848 1094 1133 1193 1653
Size: 233 615 246 39 60 460
Fragments arranged by size:
615 460 246 233 60 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1300 1653
Size: 1300 353
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1614 1653
Size: 1614 39
XmaI C'CCGG_G
Cuts at: 0 1560 1653
Size: 1560 93
Enzymes that do cut:
AarI AccI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AvaI BanII BbsI BcgI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrDI BsrFI BssSI BstEII BstXI
BstYI Bsu36I BtgI BtsI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII MmeI MscI MslI MspA1I NcoI NdeI NspI
PflMI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI SfcI
SmaI SmlI SphI StuI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BbvCI BciVI BclI BfrBI BmgBI BmtI BsaAI
BsaBI BsaHI BsiWI BsmBI BsrBI BsrGI BssHII BstAPI
BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EcoRV FalI FseI HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PsiI
PspXI PsrI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI TatI TstI Tth111I
XbaI XhoI XmnI ZraI