Restriction map for
pF1KB7852
(Linear) (Six-base) MAPSORT of: KIBB7852.seq Check: 4155 from: 1 to: 1842
KIBB7852.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1458 1842
Size: 1458 384
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 116 1129 1422 1777 1826 1842
Size: 101 15 1013 293 355 49 16
Fragments arranged by size:
1013 355 293 101 49 16 15
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 434 466 1842
Size: 434 32 1376
Fragments arranged by size:
1376 434 32
AlwNI CAG_nnn'CTG
Cuts at: 0 139 199 850 1842
Size: 139 60 651 992
Fragments arranged by size:
992 651 139 60
ApaI G_GGCC'C
Cuts at: 0 1750 1842
Size: 1750 92
ApoI r'AATT_y
Cuts at: 0 475 571 801 1281 1842
Size: 475 96 230 480 561
Fragments arranged by size:
561 480 475 230 96
AvaI C'yCGr_G
Cuts at: 0 1144 1842
Size: 1144 698
BanI G'GyrC_C
Cuts at: 0 1148 1842
Size: 1148 694
BanII G_rGCy'C
Cuts at: 0 1750 1842
Size: 1750 92
Bme1580I G_kGCm'C
Cuts at: 0 1750 1842
Size: 1750 92
BmgBI CAC'GTC
Cuts at: 0 126 1842
Size: 126 1716
BmrI ACTGGGnnnn_n'
Cuts at: 0 693 1026 1753 1842
Size: 693 333 727 89
Fragments arranged by size:
727 693 333 89
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 893 1842
Size: 893 949
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 1842
Size: 613 1229
BsaBI GATnn'nnATC
Cuts at: 0 1735 1842
Size: 1735 107
BseYI C'CCAG_C
Cuts at: 0 1749 1842
Size: 1749 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 430 1842
Size: 125 305 1412
Fragments arranged by size:
1412 305 125
BsiEI CG_ry'CG
Cuts at: 0 1052 1257 1842
Size: 1052 205 585
Fragments arranged by size:
1052 585 205
BsmI GAATG_Cn'
Cuts at: 0 1788 1842
Size: 1788 54
Bsp1286I G_dGCh'C
Cuts at: 0 1750 1842
Size: 1750 92
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1458 1842
Size: 1458 384
BsrGI T'GTAC_A
Cuts at: 0 1377 1626 1842
Size: 1377 249 216
Fragments arranged by size:
1377 249 216
BstAPI GCAn_nnn'nTGC
Cuts at: 0 850 1470 1842
Size: 850 620 372
Fragments arranged by size:
850 620 372
BstEII G'GTnAC_C
Cuts at: 0 331 1842
Size: 331 1511
BstXI CCAn_nnnn'nTGG
Cuts at: 0 419 739 743 1171 1590 1842
Size: 419 320 4 428 419 252
Fragments arranged by size:
428 419 419 320 252 4
BstYI r'GATC_y
Cuts at: 0 1112 1842
Size: 1112 730
Bsu36I CC'TnA_GG
Cuts at: 0 455 1821 1842
Size: 455 1366 21
Fragments arranged by size:
1366 455 21
BtgI C'CryG_G
Cuts at: 0 599 1170 1496 1842
Size: 599 571 326 346
Fragments arranged by size:
599 571 346 326
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 725 1842
Size: 725 1117
BtsI GCAGTG_nn'
Cuts at: 0 1545 1842
Size: 1545 297
ClaI AT'CG_AT
Cuts at: 0 1682 1842
Size: 1682 160
DraI TTT'AAA
Cuts at: 0 864 1842
Size: 864 978
EarI CTCTTCn'nnn_
Cuts at: 0 77 1302 1441 1640 1643 1842
Size: 77 1225 139 199 3 199
Fragments arranged by size:
1225 199 199 139 77 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 116 613 1129 1422 1777 1826 1842
Size: 101 15 497 516 293 355 49 16
Fragments arranged by size:
516 497 355 293 101 49 16 15
EcoO109I rG'GnC_Cy
Cuts at: 0 1521 1842
Size: 1521 321
EcoRV GAT'ATC
Cuts at: 0 1041 1842
Size: 1041 801
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1804 1836 1842
Size: 1804 32 6
Fragments arranged by size:
1804 32 6
HindIII A'AGCT_T
Cuts at: 0 25 1842
Size: 25 1817
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 1579 1842
Size: 561 1018 263
Fragments arranged by size:
1018 561 263
MslI CAynn'nnrTG
Cuts at: 0 242 353 989 1007 1169 1196 1842
Size: 242 111 636 18 162 27 646
Fragments arranged by size:
646 636 242 162 111 27 18
MspA1I CmG'CkG
Cuts at: 0 136 1842
Size: 136 1706
NspI r_CATG'y
Cuts at: 0 1657 1842
Size: 1657 185
PfoI T'CCnGG_A
Cuts at: 0 808 1842
Size: 808 1034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 965 997 1842
Size: 965 32 845
Fragments arranged by size:
965 845 32
PpuMI rG'GwC_Cy
Cuts at: 0 1521 1842
Size: 1521 321
PspOMI G'GGCC_C
Cuts at: 0 1746 1842
Size: 1746 96
PvuI CG_AT'CG
Cuts at: 0 1052 1257 1842
Size: 1052 205 585
Fragments arranged by size:
1052 585 205
SapI GCTCTTCn'nnn_
Cuts at: 0 1643 1842
Size: 1643 199
SfcI C'TryA_G
Cuts at: 0 314 1323 1842
Size: 314 1009 519
Fragments arranged by size:
1009 519 314
SmaI CCC'GGG
Cuts at: 0 1146 1842
Size: 1146 696
SpeI A'CTAG_T
Cuts at: 0 666 1842
Size: 666 1176
SphI G_CATG'C
Cuts at: 0 1657 1842
Size: 1657 185
StuI AGG'CCT
Cuts at: 0 502 1842
Size: 502 1340
StyI C'CwwG_G
Cuts at: 0 98 215 620 780 1842
Size: 98 117 405 160 1062
Fragments arranged by size:
1062 405 160 117 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1008 1842
Size: 1008 834
TatI w'GTAC_w
Cuts at: 0 1377 1626 1842
Size: 1377 249 216
Fragments arranged by size:
1377 249 216
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1059 1091 1842
Size: 1059 32 751
Fragments arranged by size:
1059 751 32
Tth111I GACn'n_nGTC
Cuts at: 0 1720 1842
Size: 1720 122
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 48 1842
Size: 48 1794
XmaI C'CCGG_G
Cuts at: 0 1144 1842
Size: 1144 698
XmnI GAAnn'nnTTC
Cuts at: 0 532 1842
Size: 532 1310
Enzymes that do cut:
AarI AcuI AloI AlwNI ApaI ApoI AvaI BanI
BanII Bme1580I BmgBI BmrI BplI BpmI BsaBI BseYI
BsgI BsiEI BsmI Bsp1286I BspMI BsrGI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
EarI Eco57MI EcoO109I EcoRV Hin4I HindIII MmeI MslI
MspA1I NspI PfoI PpiI PpuMI PspOMI PvuI SapI
SfcI SmaI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmtI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BsiHKAI BsiWI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstBI
BstZ17I CspCI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI EcoRI FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PshAI PsiI PspXI
PsrI PstI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SrfI SspI SwaI XbaI XhoI ZraI