Restriction map for
pF1KB7849
(Linear) (Six-base) MAPSORT of: KIBB7849.seq Check: 508 from: 1 to: 1635
KIBB7849.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
Acc65I G'GTAC_C
Cuts at: 0 1085 1635
Size: 1085 550
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 813 1065 1317 1401 1569 1635
Size: 813 252 252 84 168 66
Fragments arranged by size:
813 252 252 168 84 66
AflIII A'CryG_T
Cuts at: 0 687 855 1191 1611 1635
Size: 687 168 336 420 24
Fragments arranged by size:
687 420 336 168 24
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 106 138 1635
Size: 106 32 1497
Fragments arranged by size:
1497 106 32
AlwNI CAG_nnn'CTG
Cuts at: 0 595 1635
Size: 595 1040
ApaLI G'TGCA_C
Cuts at: 0 391 429 1525 1635
Size: 391 38 1096 110
Fragments arranged by size:
1096 391 110 38
BanI G'GyrC_C
Cuts at: 0 1085 1635
Size: 1085 550
BbsI GAAGACnn'nnnn_
Cuts at: 0 745 1194 1635
Size: 745 449 441
Fragments arranged by size:
745 449 441
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 432 466 1635
Size: 432 34 1169
Fragments arranged by size:
1169 432 34
BfrBI ATG'CAT
Cuts at: 0 721 1635
Size: 721 914
BglII A'GATC_T
Cuts at: 0 466 1635
Size: 466 1169
Bme1580I G_kGCm'C
Cuts at: 0 395 433 1529 1635
Size: 395 38 1096 106
Fragments arranged by size:
1096 395 106 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 296 893 977 1313 1565 1635
Size: 121 175 597 84 336 252 70
Fragments arranged by size:
597 336 252 175 121 84 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 387 567 1635
Size: 387 180 1068
Fragments arranged by size:
1068 387 180
BsaI GGTCTCn'nnnn_
Cuts at: 0 97 355 477 1545 1635
Size: 97 258 122 1068 90
Fragments arranged by size:
1068 258 122 97 90
BsaAI yAC'GTr
Cuts at: 0 606 774 942 1278 1530 1635
Size: 606 168 168 336 252 105
Fragments arranged by size:
606 336 252 168 168 105
BsaWI w'CCGG_w
Cuts at: 0 1123 1635
Size: 1123 512
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 106 136 315 345 1635
Size: 106 30 179 30 1290
Fragments arranged by size:
1290 179 106 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 90 915 1083 1251 1335 1635
Size: 90 825 168 168 84 300
Fragments arranged by size:
825 300 168 168 90 84
BseYI C'CCAG_C
Cuts at: 0 318 1635
Size: 318 1317
BsiHKAI G_wGCw'C
Cuts at: 0 395 433 1107 1529 1635
Size: 395 38 674 422 106
Fragments arranged by size:
674 422 395 106 38
BsiWI C'GTAC_G
Cuts at: 0 1388 1635
Size: 1388 247
BsmI GAATG_Cn'
Cuts at: 0 1072 1121 1567 1635
Size: 1072 49 446 68
Fragments arranged by size:
1072 446 68 49
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1016 1635
Size: 1016 619
Bsp1286I G_dGCh'C
Cuts at: 0 395 433 1107 1529 1635
Size: 395 38 674 422 106
Fragments arranged by size:
674 422 395 106 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 115 1635
Size: 115 1520
BsrDI GCAATG_nn'
Cuts at: 0 55 981 1635
Size: 55 926 654
Fragments arranged by size:
926 654 55
BstBI TT'CG_AA
Cuts at: 0 1269 1635
Size: 1269 366
BstXI CCAn_nnnn'nTGG
Cuts at: 0 50 1635
Size: 50 1585
BstYI r'GATC_y
Cuts at: 0 466 1635
Size: 466 1169
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1583 1635
Size: 1583 52
BtsI GCAGTG_nn'
Cuts at: 0 1156 1576 1635
Size: 1156 420 59
Fragments arranged by size:
1156 420 59
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1134 1169 1554 1589 1635
Size: 1134 35 385 35 46
Fragments arranged by size:
1134 385 46 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 437 1459 1635
Size: 437 1022 176
Fragments arranged by size:
1022 437 176
EarI CTCTTCn'nnn_
Cuts at: 0 41 1019 1635
Size: 41 978 616
Fragments arranged by size:
978 616 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 296 813 893 977 1065 1313 1317
Size: 121 175 517 80 84 88 248 4
Cuts at: 1317 1401 1565 1569 1635
Size: 84 164 4 66
Fragments arranged by size:
517 248 175 164 121 88 84 84
80 66 4 4
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 223 255 842 874 1635
Size: 223 32 587 32 761
Fragments arranged by size:
761 587 223 32 32
HindIII A'AGCT_T
Cuts at: 0 656 1635
Size: 656 979
KpnI G_GTAC'C
Cuts at: 0 1089 1635
Size: 1089 546
MslI CAynn'nnrTG
Cuts at: 0 1114 1635
Size: 1114 521
MspA1I CmG'CkG
Cuts at: 0 318 515 833 917 1208 1337 1376 1635
Size: 318 197 318 84 291 129 39 259
Fragments arranged by size:
318 318 291 259 197 129 84 39
NsiI A_TGCA'T
Cuts at: 0 723 1635
Size: 723 912
NspI r_CATG'y
Cuts at: 0 691 725 859 1195 1363 1447 1615 1627
Size: 691 34 134 336 168 84 168 12
Cuts at: 1627 1635
Size: 8
Fragments arranged by size:
691 336 168 168 134 84 34 12
8
PciI A'CATG_T
Cuts at: 0 687 855 1191 1611 1635
Size: 687 168 336 420 24
Fragments arranged by size:
687 420 336 168 24
PmlI CAC'GTG
Cuts at: 0 942 1278 1530 1635
Size: 942 336 252 105
Fragments arranged by size:
942 336 252 105
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 1635
Size: 106 32 1497
Fragments arranged by size:
1497 106 32
PstI C_TGCA'G
Cuts at: 0 112 514 1635
Size: 112 402 1121
Fragments arranged by size:
1121 402 112
PvuII CAG'CTG
Cuts at: 0 318 515 833 917 1337 1635
Size: 318 197 318 84 420 298
Fragments arranged by size:
420 318 318 298 197 84
SbfI CC_TGCA'GG
Cuts at: 0 112 1635
Size: 112 1523
ScaI AGT'ACT
Cuts at: 0 1432 1635
Size: 1432 203
SfcI C'TryA_G
Cuts at: 0 84 108 510 554 1248 1500 1635
Size: 84 24 402 44 694 252 135
Fragments arranged by size:
694 402 252 135 84 44 24
SmlI C'TyrA_G
Cuts at: 0 366 582 1635
Size: 366 216 1053
Fragments arranged by size:
1053 366 216
StuI AGG'CCT
Cuts at: 0 1162 1635
Size: 1162 473
StyI C'CwwG_G
Cuts at: 0 125 1635
Size: 125 1510
TatI w'GTAC_w
Cuts at: 0 1430 1480 1635
Size: 1430 50 155
Fragments arranged by size:
1430 155 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 315 347 1635
Size: 315 32 1288
Fragments arranged by size:
1288 315 32
XmnI GAAnn'nnTTC
Cuts at: 0 231 826 1162 1330 1414 1582 1635
Size: 231 595 336 168 84 168 53
Fragments arranged by size:
595 336 231 168 168 84 53
Enzymes that do cut:
Acc65I AcuI AflIII AloI AlwNI ApaLI BanI BbsI
BcgI BfrBI BglII Bme1580I BpmI BpuEI BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspMI BsrDI BstBI BstXI BstYI BtgZI BtsI
CspCI DraIII EarI Eco57MI Hin4I HindIII KpnI MslI
MspA1I NsiI NspI PciI PmlI PpiI PstI PvuII
SbfI ScaI SfcI SmlI StuI StyI TatI TstI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbvCI BciVI BclI
BglI BlpI BmgBI BmrI BmtI BplI Bpu10I BsaBI
BsaHI BsgI BsiEI BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtgI ClaI
DraI DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PflMI PfoI PmeI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI ZraI