(Linear) (Six-base) MAPSORT of: KIBB7842.seq Check: 3105 from: 1 to: 1851 KIBB7842.orf With 182 enzymes: SgfI * October 7, 2009 14:24 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 71 171 874 1191 1851 Size: 71 100 703 317 660 Fragments arranged by size: 703 660 317 100 71 AflIII A'CryG_T Cuts at: 0 595 1024 1851 Size: 595 429 827 Fragments arranged by size: 827 595 429 AleI CACnn'nnGTG Cuts at: 0 1418 1851 Size: 1418 433 ApoI r'AATT_y Cuts at: 0 43 294 700 1299 1636 1801 1851 Size: 43 251 406 599 337 165 50 Fragments arranged by size: 599 406 337 251 165 50 43 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1617 1650 1851 Size: 1617 33 201 Fragments arranged by size: 1617 201 33 BanII G_rGCy'C Cuts at: 0 715 1851 Size: 715 1136 BbsI GAAGACnn'nnnn_ Cuts at: 0 105 277 1207 1851 Size: 105 172 930 644 Fragments arranged by size: 930 644 172 105 BglII A'GATC_T Cuts at: 0 1790 1851 Size: 1790 61 BmrI ACTGGGnnnn_n' Cuts at: 0 923 1851 Size: 923 928 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1562 1812 1851 Size: 1562 250 39 Fragments arranged by size: 1562 250 39 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 767 1607 1796 1851 Size: 767 840 189 55 Fragments arranged by size: 840 767 189 55 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1414 1851 Size: 1414 437 BsaI GGTCTCn'nnnn_ Cuts at: 0 410 1419 1851 Size: 410 1009 432 Fragments arranged by size: 1009 432 410 BsaAI yAC'GTr Cuts at: 0 492 1851 Size: 492 1359 BsaBI GATnn'nnATC Cuts at: 0 560 1851 Size: 560 1291 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 136 139 1372 1851 Size: 136 3 1233 479 Fragments arranged by size: 1233 479 136 3 BseYI C'CCAG_C Cuts at: 0 129 630 1463 1715 1851 Size: 129 501 833 252 136 Fragments arranged by size: 833 501 252 136 129 BsiHKAI G_wGCw'C Cuts at: 0 715 1851 Size: 715 1136 BsmI GAATG_Cn' Cuts at: 0 981 1851 Size: 981 870 Bsp1286I G_dGCh'C Cuts at: 0 715 1851 Size: 715 1136 BspHI T'CATG_A Cuts at: 0 876 1851 Size: 876 975 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 203 1851 Size: 203 1648 BsrDI GCAATG_nn' Cuts at: 0 778 813 1114 1851 Size: 778 35 301 737 Fragments arranged by size: 778 737 301 35 BstYI r'GATC_y Cuts at: 0 308 1324 1389 1790 1851 Size: 308 1016 65 401 61 Fragments arranged by size: 1016 401 308 65 61 BtgI C'CryG_G Cuts at: 0 986 1070 1851 Size: 986 84 781 Fragments arranged by size: 986 781 84 BtsI GCAGTG_nn' Cuts at: 0 92 1618 1851 Size: 92 1526 233 Fragments arranged by size: 1526 233 92 EarI CTCTTCn'nnn_ Cuts at: 0 347 1079 1520 1781 1851 Size: 347 732 441 261 70 Fragments arranged by size: 732 441 347 261 70 EcoICRI GAG'CTC Cuts at: 0 713 1851 Size: 713 1138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 71 171 767 874 1191 1607 1796 1851 Size: 71 100 596 107 317 416 189 55 Fragments arranged by size: 596 416 317 189 107 100 71 55 EcoRI G'AATT_C Cuts at: 0 294 1801 1851 Size: 294 1507 50 Fragments arranged by size: 1507 294 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 293 325 702 734 1374 1406 1851 Size: 293 32 377 32 640 32 445 Fragments arranged by size: 640 445 377 293 32 32 32 HindIII A'AGCT_T Cuts at: 0 615 1851 Size: 615 1236 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1367 1851 Size: 1367 484 MslI CAynn'nnrTG Cuts at: 0 596 930 1014 1098 1418 1851 Size: 596 334 84 84 320 433 Fragments arranged by size: 596 433 334 320 84 84 NcoI C'CATG_G Cuts at: 0 986 1070 1851 Size: 986 84 781 Fragments arranged by size: 986 781 84 NdeI CA'TA_TG Cuts at: 0 659 1851 Size: 659 1192 NspI r_CATG'y Cuts at: 0 599 1028 1580 1653 1851 Size: 599 429 552 73 198 Fragments arranged by size: 599 552 429 198 73 PciI A'CATG_T Cuts at: 0 595 1024 1851 Size: 595 429 827 Fragments arranged by size: 827 595 429 PmlI CAC'GTG Cuts at: 0 492 1851 Size: 492 1359 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 184 216 1851 Size: 184 32 1635 Fragments arranged by size: 1635 184 32 PsiI TTA'TAA Cuts at: 0 845 1851 Size: 845 1006 SacI G_AGCT'C Cuts at: 0 715 1851 Size: 715 1136 SexAI A'CCwGG_T Cuts at: 0 400 1851 Size: 400 1451 SmlI C'TyrA_G Cuts at: 0 1393 1851 Size: 1393 458 StyI C'CwwG_G Cuts at: 0 986 1070 1851 Size: 986 84 781 Fragments arranged by size: 986 781 84 TatI w'GTAC_w Cuts at: 0 1552 1851 Size: 1552 299 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 353 1851 Size: 353 1498 XmnI GAAnn'nnTTC Cuts at: 0 23 757 784 868 925 1204 1851 Size: 23 734 27 84 57 279 647 Fragments arranged by size: 734 647 279 84 57 27 23 Enzymes that do cut: AcuI AflIII AleI ApoI BaeI BanII BbsI BglII BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BstYI BtgI BtsI EarI EcoICRI Eco57MI EcoRI Hin4I HindIII MmeI MslI NcoI NdeI NspI PciI PmlI PpiI PsiI SacI SexAI SmlI StyI TatI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI Bpu10I BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XhoI XmaI ZraI