Restriction map for pF1KB7842
 (Linear) (Six-base) MAPSORT of: KIBB7842.seq  Check: 3105  from: 1  to: 1851



KIBB7842.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     171     874    1191    1851 
   Size:         71     100     703     317     660

  Fragments arranged by size: 

                 703     660     317     100      71
 
AflIII A'CryG_T

Cuts at:      0     595    1024    1851 
   Size:        595     429     827

  Fragments arranged by size: 

                 827     595     429
 
AleI CACnn'nnGTG

Cuts at:      0    1418    1851 
   Size:       1418     433
 
ApoI r'AATT_y

Cuts at:      0      43     294     700    1299    1636    1801    1851 
   Size:         43     251     406     599     337     165      50

  Fragments arranged by size: 

                 599     406     337     251     165      50      43
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1617    1650    1851 
   Size:       1617      33     201

  Fragments arranged by size: 

                1617     201      33
 
BanII G_rGCy'C

Cuts at:      0     715    1851 
   Size:        715    1136
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     105     277    1207    1851 
   Size:        105     172     930     644

  Fragments arranged by size: 

                 930     644     172     105
 
BglII A'GATC_T

Cuts at:      0    1790    1851 
   Size:       1790      61
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     923    1851 
   Size:        923     928
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1562    1812    1851 
   Size:       1562     250      39

  Fragments arranged by size: 

                1562     250      39
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     767    1607    1796    1851 
   Size:        767     840     189      55

  Fragments arranged by size: 

                 840     767     189      55
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1414    1851 
   Size:       1414     437
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     410    1419    1851 
   Size:        410    1009     432

  Fragments arranged by size: 

                1009     432     410
 
BsaAI yAC'GTr

Cuts at:      0     492    1851 
   Size:        492    1359
 
BsaBI GATnn'nnATC

Cuts at:      0     560    1851 
   Size:        560    1291
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     136     139    1372    1851 
   Size:        136       3    1233     479

  Fragments arranged by size: 

                1233     479     136       3
 
BseYI C'CCAG_C

Cuts at:      0     129     630    1463    1715    1851 
   Size:        129     501     833     252     136

  Fragments arranged by size: 

                 833     501     252     136     129
 
BsiHKAI G_wGCw'C

Cuts at:      0     715    1851 
   Size:        715    1136
 
BsmI GAATG_Cn'

Cuts at:      0     981    1851 
   Size:        981     870
 
Bsp1286I G_dGCh'C

Cuts at:      0     715    1851 
   Size:        715    1136
 
BspHI T'CATG_A

Cuts at:      0     876    1851 
   Size:        876     975
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     203    1851 
   Size:        203    1648
 
BsrDI GCAATG_nn'

Cuts at:      0     778     813    1114    1851 
   Size:        778      35     301     737

  Fragments arranged by size: 

                 778     737     301      35
 
BstYI r'GATC_y

Cuts at:      0     308    1324    1389    1790    1851 
   Size:        308    1016      65     401      61

  Fragments arranged by size: 

                1016     401     308      65      61
 
BtgI C'CryG_G

Cuts at:      0     986    1070    1851 
   Size:        986      84     781

  Fragments arranged by size: 

                 986     781      84
 
BtsI GCAGTG_nn'

Cuts at:      0      92    1618    1851 
   Size:         92    1526     233

  Fragments arranged by size: 

                1526     233      92
 
EarI CTCTTCn'nnn_

Cuts at:      0     347    1079    1520    1781    1851 
   Size:        347     732     441     261      70

  Fragments arranged by size: 

                 732     441     347     261      70
 
EcoICRI GAG'CTC

Cuts at:      0     713    1851 
   Size:        713    1138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     171     767     874    1191    1607    1796    1851 
   Size:         71     100     596     107     317     416     189      55

  Fragments arranged by size: 

                 596     416     317     189     107     100      71      55
            
 
EcoRI G'AATT_C

Cuts at:      0     294    1801    1851 
   Size:        294    1507      50

  Fragments arranged by size: 

                1507     294      50
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     293     325     702     734    1374    1406    1851 
   Size:        293      32     377      32     640      32     445

  Fragments arranged by size: 

                 640     445     377     293      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     615    1851 
   Size:        615    1236
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1367    1851 
   Size:       1367     484
 
MslI CAynn'nnrTG

Cuts at:      0     596     930    1014    1098    1418    1851 
   Size:        596     334      84      84     320     433

  Fragments arranged by size: 

                 596     433     334     320      84      84
 
NcoI C'CATG_G

Cuts at:      0     986    1070    1851 
   Size:        986      84     781

  Fragments arranged by size: 

                 986     781      84
 
NdeI CA'TA_TG

Cuts at:      0     659    1851 
   Size:        659    1192
 
NspI r_CATG'y

Cuts at:      0     599    1028    1580    1653    1851 
   Size:        599     429     552      73     198

  Fragments arranged by size: 

                 599     552     429     198      73
 
PciI A'CATG_T

Cuts at:      0     595    1024    1851 
   Size:        595     429     827

  Fragments arranged by size: 

                 827     595     429
 
PmlI CAC'GTG

Cuts at:      0     492    1851 
   Size:        492    1359
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     184     216    1851 
   Size:        184      32    1635

  Fragments arranged by size: 

                1635     184      32
 
PsiI TTA'TAA

Cuts at:      0     845    1851 
   Size:        845    1006
 
SacI G_AGCT'C

Cuts at:      0     715    1851 
   Size:        715    1136
 
SexAI A'CCwGG_T

Cuts at:      0     400    1851 
   Size:        400    1451
 
SmlI C'TyrA_G

Cuts at:      0    1393    1851 
   Size:       1393     458
 
StyI C'CwwG_G

Cuts at:      0     986    1070    1851 
   Size:        986      84     781

  Fragments arranged by size: 

                 986     781      84
 
TatI w'GTAC_w

Cuts at:      0    1552    1851 
   Size:       1552     299
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     353    1851 
   Size:        353    1498
 
XmnI GAAnn'nnTTC

Cuts at:      0      23     757     784     868     925    1204    1851 
   Size:         23     734      27      84      57     279     647

  Fragments arranged by size: 

                 734     647     279      84      57      27      23
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     ApoI     BaeI    BanII     BbsI    BglII
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BseRI
    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BstYI
     BtgI     BtsI     EarI  EcoICRI  Eco57MI    EcoRI    Hin4I  HindIII
     MmeI     MslI     NcoI     NdeI     NspI     PciI     PmlI     PpiI
     PsiI     SacI    SexAI     SmlI     StyI     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI
   Bpu10I    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI    BsmBI
    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI    EcoNI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI
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