Restriction map for
pF1KB7841
(Linear) (Six-base) MAPSORT of: KIBB7841.seq Check: 1081 from: 1 to: 1512
KIBB7841.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 974 1174 1512
Size: 974 200 338
Fragments arranged by size:
974 338 200
AgeI A'CCGG_T
Cuts at: 0 1396 1512
Size: 1396 116
AleI CACnn'nnGTG
Cuts at: 0 596 1512
Size: 596 916
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1234 1237 1256 1512
Size: 1234 3 19 256
Fragments arranged by size:
1234 256 19 3
AlwNI CAG_nnn'CTG
Cuts at: 0 87 1512
Size: 87 1425
ApaLI G'TGCA_C
Cuts at: 0 46 1512
Size: 46 1466
ApoI r'AATT_y
Cuts at: 0 278 511 574 980 1112 1133 1434 1512
Size: 278 233 63 406 132 21 301 78
Fragments arranged by size:
406 301 278 233 132 78 63 21
AseI AT'TA_AT
Cuts at: 0 1370 1512
Size: 1370 142
AvaI C'yCGr_G
Cuts at: 0 69 1512
Size: 69 1443
BamHI G'GATC_C
Cuts at: 0 620 1512
Size: 620 892
BanI G'GyrC_C
Cuts at: 0 91 1072 1272 1512
Size: 91 981 200 240
Fragments arranged by size:
981 240 200 91
BbeI G_GCGC'C
Cuts at: 0 95 1512
Size: 95 1417
BclI T'GATC_A
Cuts at: 0 1419 1512
Size: 1419 93
BglI GCCn_nnn'nGGC
Cuts at: 0 71 1512
Size: 71 1441
Bme1580I G_kGCm'C
Cuts at: 0 50 614 1275 1512
Size: 50 564 661 237
Fragments arranged by size:
661 564 237 50
BmrI ACTGGGnnnn_n'
Cuts at: 0 97 1512
Size: 97 1415
BmtI G_CTAG'C
Cuts at: 0 686 1512
Size: 686 826
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 782 1512
Size: 782 730
BsaHI Gr'CG_yC
Cuts at: 0 59 92 1512
Size: 59 33 1420
Fragments arranged by size:
1420 59 33
BsaWI w'CCGG_w
Cuts at: 0 1396 1512
Size: 1396 116
BseYI C'CCAG_C
Cuts at: 0 525 870 1512
Size: 525 345 642
Fragments arranged by size:
642 525 345
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 1142 1512
Size: 737 405 370
Fragments arranged by size:
737 405 370
BsiHKAI G_wGCw'C
Cuts at: 0 14 50 182 904 1512
Size: 14 36 132 722 608
Fragments arranged by size:
722 608 132 36 14
Bsp1286I G_dGCh'C
Cuts at: 0 14 50 182 614 904 1275 1512
Size: 14 36 132 432 290 371 237
Fragments arranged by size:
432 371 290 237 132 36 14
BspHI T'CATG_A
Cuts at: 0 768 1512
Size: 768 744
BsrDI GCAATG_nn'
Cuts at: 0 124 1021 1512
Size: 124 897 491
Fragments arranged by size:
897 491 124
BsrFI r'CCGG_y
Cuts at: 0 94 1396 1512
Size: 94 1302 116
Fragments arranged by size:
1302 116 94
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1020 1249 1512
Size: 1020 229 263
Fragments arranged by size:
1020 263 229
BstYI r'GATC_y
Cuts at: 0 620 1512
Size: 620 892
BtgI C'CryG_G
Cuts at: 0 583 826 1512
Size: 583 243 686
Fragments arranged by size:
686 583 243
BtsI GCAGTG_nn'
Cuts at: 0 558 915 1254 1272 1512
Size: 558 357 339 18 240
Fragments arranged by size:
558 357 339 240 18
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 443 478 1512
Size: 443 35 1034
Fragments arranged by size:
1034 443 35
DraIII CAC_nnn'GTG
Cuts at: 0 818 1512
Size: 818 694
EarI CTCTTCn'nnn_
Cuts at: 0 751 1044 1512
Size: 751 293 468
Fragments arranged by size:
751 468 293
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 974 1174 1512
Size: 974 200 338
Fragments arranged by size:
974 338 200
EcoO109I rG'GnC_Cy
Cuts at: 0 522 1390 1512
Size: 522 868 122
Fragments arranged by size:
868 522 122
EcoRI G'AATT_C
Cuts at: 0 1133 1512
Size: 1133 379
HaeII r_GCGC'y
Cuts at: 0 95 101 877 1512
Size: 95 6 776 635
Fragments arranged by size:
776 635 95 6
HincII GTy'rAC
Cuts at: 0 630 1512
Size: 630 882
KasI G'GCGC_C
Cuts at: 0 91 1512
Size: 91 1421
MslI CAynn'nnrTG
Cuts at: 0 596 1014 1512
Size: 596 418 498
Fragments arranged by size:
596 498 418
NaeI GCC'GGC
Cuts at: 0 96 1512
Size: 96 1416
NarI GG'CG_CC
Cuts at: 0 92 1512
Size: 92 1420
NdeI CA'TA_TG
Cuts at: 0 273 1512
Size: 273 1239
NgoMIV G'CCGG_C
Cuts at: 0 94 1512
Size: 94 1418
NheI G'CTAG_C
Cuts at: 0 682 1512
Size: 682 830
PflMI CCAn_nnn'nTGG
Cuts at: 0 166 1512
Size: 166 1346
PfoI T'CCnGG_A
Cuts at: 0 1001 1512
Size: 1001 511
PpuMI rG'GwC_Cy
Cuts at: 0 522 1390 1512
Size: 522 868 122
Fragments arranged by size:
868 522 122
SanDI GG'GwC_CC
Cuts at: 0 522 1390 1512
Size: 522 868 122
Fragments arranged by size:
868 522 122
SfoI GGC'GCC
Cuts at: 0 93 1512
Size: 93 1419
SgrAI Cr'CCGG_yG
Cuts at: 0 94 1512
Size: 94 1418
SmlI C'TyrA_G
Cuts at: 0 13 1512
Size: 13 1499
StyI C'CwwG_G
Cuts at: 0 261 1512
Size: 261 1251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 197 295 755 1512
Size: 197 98 460 757
Fragments arranged by size:
757 460 197 98
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 291 323 1512
Size: 291 32 1189
Fragments arranged by size:
1189 291 32
Tth111I GACn'n_nGTC
Cuts at: 0 1388 1512
Size: 1388 124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 159 1512
Size: 159 1353
XmnI GAAnn'nnTTC
Cuts at: 0 980 1512
Size: 980 532
Enzymes that do cut:
AcuI AgeI AleI AlfI AlwNI ApaLI ApoI AseI
AvaI BamHI BanI BbeI BclI BglI Bme1580I BmrI
BmtI BplI BsaHI BsaWI BseYI BsgI BsiHKAI Bsp1286I
BspHI BsrDI BsrFI BstAPI BstYI BtgI BtsI CspCI
DraIII EarI Eco57MI EcoO109I EcoRI HaeII HincII KasI
MslI NaeI NarI NdeI NgoMIV NheI PflMI PfoI
PpuMI SanDI SfoI SgrAI SmlI StyI TaqII TstI
Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AloI ApaI AscI AsiSI AvrII BaeI BanII
BbsI BbvCI BcgI BciVI BfrBI BglII BlpI BmgBI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI DraI DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI
KpnI MfeI MluI MmeI MscI MspA1I NcoI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI XbaI XhoI XmaI
ZraI