(Linear) (Six-base) MAPSORT of: KIBB7837.seq Check: 9456 from: 1 to: 1620 KIBB7837.orf With 182 enzymes: SgfI * June 26, 2008 10:50 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 429 849 933 1353 1601 1620 Size: 429 420 84 420 248 19 Fragments arranged by size: 429 420 420 248 84 19 AflIII A'CryG_T Cuts at: 0 421 1565 1620 Size: 421 1144 55 Fragments arranged by size: 1144 421 55 AleI CACnn'nnGTG Cuts at: 0 992 1412 1620 Size: 992 420 208 Fragments arranged by size: 992 420 208 AlwNI CAG_nnn'CTG Cuts at: 0 273 1620 Size: 273 1347 ApoI r'AATT_y Cuts at: 0 245 815 983 1620 Size: 245 570 168 637 Fragments arranged by size: 637 570 245 168 BbsI GAAGACnn'nnnn_ Cuts at: 0 558 726 1146 1620 Size: 558 168 420 474 Fragments arranged by size: 558 474 420 168 BciVI GTATCCnnnnn_n' Cuts at: 0 1135 1620 Size: 1135 485 BfrBI ATG'CAT Cuts at: 0 1604 1620 Size: 1604 16 BglII A'GATC_T Cuts at: 0 1036 1456 1620 Size: 1036 420 164 Fragments arranged by size: 1036 420 164 BmrI ACTGGGnnnn_n' Cuts at: 0 617 1620 Size: 617 1003 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 316 514 1620 Size: 316 198 1106 Fragments arranged by size: 1106 316 198 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 311 509 677 761 845 929 1097 1181 Size: 311 198 168 84 84 84 168 84 Cuts at: 1181 1265 1349 1620 Size: 84 84 271 Fragments arranged by size: 311 271 198 168 168 84 84 84 84 84 84 BsaAI yAC'GTr Cuts at: 0 424 1620 Size: 424 1196 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 61 1620 Size: 61 1559 BsmI GAATG_Cn' Cuts at: 0 931 1114 1620 Size: 931 183 506 Fragments arranged by size: 931 506 183 BsrDI GCAATG_nn' Cuts at: 0 856 1360 1512 1620 Size: 856 504 152 108 Fragments arranged by size: 856 504 152 108 BstBI TT'CG_AA Cuts at: 0 465 633 717 1221 1305 1389 1620 Size: 465 168 84 504 84 84 231 Fragments arranged by size: 504 465 231 168 84 84 84 BstYI r'GATC_y Cuts at: 0 63 1036 1456 1620 Size: 63 973 420 164 Fragments arranged by size: 973 420 164 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1452 1620 Size: 1452 168 BtsI GCAGTG_nn' Cuts at: 0 940 1620 Size: 940 680 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 918 953 1506 1541 1620 Size: 918 35 553 35 79 Fragments arranged by size: 918 553 79 35 35 DraI TTT'AAA Cuts at: 0 840 1620 Size: 840 780 DraIII CAC_nnn'GTG Cuts at: 0 571 1620 Size: 571 1049 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 311 429 509 677 761 845 849 929 Size: 311 118 80 168 84 84 4 80 Cuts at: 929 933 1097 1181 1265 1349 1353 1601 1620 Size: 4 164 84 84 84 4 248 19 Fragments arranged by size: 311 248 168 164 118 84 84 84 84 84 80 80 19 4 4 4 EcoO109I rG'GnC_Cy Cuts at: 0 1168 1620 Size: 1168 452 EcoRI G'AATT_C Cuts at: 0 815 983 1620 Size: 815 168 637 Fragments arranged by size: 815 637 168 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 545 577 1620 Size: 545 32 1043 Fragments arranged by size: 1043 545 32 HincII GTy'rAC Cuts at: 0 1571 1620 Size: 1571 49 HindIII A'AGCT_T Cuts at: 0 608 1280 1620 Size: 608 672 340 Fragments arranged by size: 672 608 340 HpaI GTT'AAC Cuts at: 0 1571 1620 Size: 1571 49 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 415 1620 Size: 415 1205 MslI CAynn'nnrTG Cuts at: 0 588 992 1412 1620 Size: 588 404 420 208 Fragments arranged by size: 588 420 404 208 NdeI CA'TA_TG Cuts at: 0 197 1620 Size: 197 1423 NsiI A_TGCA'T Cuts at: 0 1606 1620 Size: 1606 14 NspI r_CATG'y Cuts at: 0 1569 1579 1608 1620 Size: 1569 10 29 12 Fragments arranged by size: 1569 29 12 10 PciI A'CATG_T Cuts at: 0 1565 1620 Size: 1565 55 PpuMI rG'GwC_Cy Cuts at: 0 1168 1620 Size: 1168 452 PsiI TTA'TAA Cuts at: 0 755 1175 1598 1620 Size: 755 420 423 22 Fragments arranged by size: 755 423 420 22 SfcI C'TryA_G Cuts at: 0 84 125 1620 Size: 84 41 1495 Fragments arranged by size: 1495 84 41 StuI AGG'CCT Cuts at: 0 946 1620 Size: 946 674 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 55 1620 Size: 55 1565 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 114 1620 Size: 114 1506 XmnI GAAnn'nnTTC Cuts at: 0 442 815 946 983 1003 1366 1403 1620 Size: 442 373 131 37 20 363 37 217 Fragments arranged by size: 442 373 363 217 131 37 37 20 Enzymes that do cut: AcuI AflIII AleI AlwNI ApoI BbsI BciVI BfrBI BglII BmrI BplI BpmI BsaAI BseRI BsmI BsrDI BstBI BstYI BtgZI BtsI CspCI DraI DraIII Eco57MI EcoO109I EcoRI Hin4I HincII HindIII HpaI MmeI MslI NdeI NsiI NspI PciI PpuMI PsiI SfcI StuI TaqII XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI BmtI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgI ClaI DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII KasI KpnI MfeI MluI MscI MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI TatI TstI Tth111I XbaI XhoI XmaI ZraI