Restriction map for pF1KB7837
 (Linear) (Six-base) MAPSORT of: KIBB7837.seq  Check: 9456  from: 1  to: 1620



KIBB7837.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     429     849     933    1353    1601    1620 
   Size:        429     420      84     420     248      19

  Fragments arranged by size: 

                 429     420     420     248      84      19
 
AflIII A'CryG_T

Cuts at:      0     421    1565    1620 
   Size:        421    1144      55

  Fragments arranged by size: 

                1144     421      55
 
AleI CACnn'nnGTG

Cuts at:      0     992    1412    1620 
   Size:        992     420     208

  Fragments arranged by size: 

                 992     420     208
 
AlwNI CAG_nnn'CTG

Cuts at:      0     273    1620 
   Size:        273    1347
 
ApoI r'AATT_y

Cuts at:      0     245     815     983    1620 
   Size:        245     570     168     637

  Fragments arranged by size: 

                 637     570     245     168
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     558     726    1146    1620 
   Size:        558     168     420     474

  Fragments arranged by size: 

                 558     474     420     168
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1135    1620 
   Size:       1135     485
 
BfrBI ATG'CAT

Cuts at:      0    1604    1620 
   Size:       1604      16
 
BglII A'GATC_T

Cuts at:      0    1036    1456    1620 
   Size:       1036     420     164

  Fragments arranged by size: 

                1036     420     164
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     617    1620 
   Size:        617    1003
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     316     514    1620 
   Size:        316     198    1106

  Fragments arranged by size: 

                1106     316     198
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     311     509     677     761     845     929    1097    1181 
   Size:        311     198     168      84      84      84     168      84

Cuts at:   1181    1265    1349    1620 
   Size:         84      84     271

  Fragments arranged by size: 

                 311     271     198     168     168      84      84      84
                  84      84      84
 
BsaAI yAC'GTr

Cuts at:      0     424    1620 
   Size:        424    1196
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61    1620 
   Size:         61    1559
 
BsmI GAATG_Cn'

Cuts at:      0     931    1114    1620 
   Size:        931     183     506

  Fragments arranged by size: 

                 931     506     183
 
BsrDI GCAATG_nn'

Cuts at:      0     856    1360    1512    1620 
   Size:        856     504     152     108

  Fragments arranged by size: 

                 856     504     152     108
 
BstBI TT'CG_AA

Cuts at:      0     465     633     717    1221    1305    1389    1620 
   Size:        465     168      84     504      84      84     231

  Fragments arranged by size: 

                 504     465     231     168      84      84      84
 
BstYI r'GATC_y

Cuts at:      0      63    1036    1456    1620 
   Size:         63     973     420     164

  Fragments arranged by size: 

                 973     420     164      63
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1452    1620 
   Size:       1452     168
 
BtsI GCAGTG_nn'

Cuts at:      0     940    1620 
   Size:        940     680
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     918     953    1506    1541    1620 
   Size:        918      35     553      35      79

  Fragments arranged by size: 

                 918     553      79      35      35
 
DraI TTT'AAA

Cuts at:      0     840    1620 
   Size:        840     780
 
DraIII CAC_nnn'GTG

Cuts at:      0     571    1620 
   Size:        571    1049
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     311     429     509     677     761     845     849     929 
   Size:        311     118      80     168      84      84       4      80

Cuts at:    929     933    1097    1181    1265    1349    1353    1601    1620 
   Size:          4     164      84      84      84       4     248      19

  Fragments arranged by size: 

                 311     248     168     164     118      84      84      84
                  84      84      80      80      19       4       4       4
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1168    1620 
   Size:       1168     452
 
EcoRI G'AATT_C

Cuts at:      0     815     983    1620 
   Size:        815     168     637

  Fragments arranged by size: 

                 815     637     168
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     545     577    1620 
   Size:        545      32    1043

  Fragments arranged by size: 

                1043     545      32
 
HincII GTy'rAC

Cuts at:      0    1571    1620 
   Size:       1571      49
 
HindIII A'AGCT_T

Cuts at:      0     608    1280    1620 
   Size:        608     672     340

  Fragments arranged by size: 

                 672     608     340
 
HpaI GTT'AAC

Cuts at:      0    1571    1620 
   Size:       1571      49
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     415    1620 
   Size:        415    1205
 
MslI CAynn'nnrTG

Cuts at:      0     588     992    1412    1620 
   Size:        588     404     420     208

  Fragments arranged by size: 

                 588     420     404     208
 
NdeI CA'TA_TG

Cuts at:      0     197    1620 
   Size:        197    1423
 
NsiI A_TGCA'T

Cuts at:      0    1606    1620 
   Size:       1606      14
 
NspI r_CATG'y

Cuts at:      0    1569    1579    1608    1620 
   Size:       1569      10      29      12

  Fragments arranged by size: 

                1569      29      12      10
 
PciI A'CATG_T

Cuts at:      0    1565    1620 
   Size:       1565      55
 
PpuMI rG'GwC_Cy

Cuts at:      0    1168    1620 
   Size:       1168     452
 
PsiI TTA'TAA

Cuts at:      0     755    1175    1598    1620 
   Size:        755     420     423      22

  Fragments arranged by size: 

                 755     423     420      22
 
SfcI C'TryA_G

Cuts at:      0      84     125    1620 
   Size:         84      41    1495

  Fragments arranged by size: 

                1495      84      41
 
StuI AGG'CCT

Cuts at:      0     946    1620 
   Size:        946     674
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      55    1620 
   Size:         55    1565
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     114    1620 
   Size:        114    1506
 
XmnI GAAnn'nnTTC

Cuts at:      0     442     815     946     983    1003    1366    1403    1620 
   Size:        442     373     131      37      20     363      37     217

  Fragments arranged by size: 

                 442     373     363     217     131      37      37      20
            
 

 Enzymes that do cut:

     AcuI   AflIII     AleI    AlwNI     ApoI     BbsI    BciVI    BfrBI
    BglII     BmrI     BplI     BpmI    BsaAI    BseRI     BsmI    BsrDI
    BstBI    BstYI    BtgZI     BtsI    CspCI     DraI   DraIII  Eco57MI
 EcoO109I    EcoRI    Hin4I   HincII  HindIII     HpaI     MmeI     MslI
     NdeI     NsiI     NspI     PciI    PpuMI     PsiI     SfcI     StuI
    TaqII     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI     BclI     BglI     BlpI Bme1580I    BmgBI     BmtI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI     ClaI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     ZraI
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