Restriction map for
pF1KB7836
(Linear) (Six-base) MAPSORT of: KIBB7836.seq Check: 7411 from: 1 to: 1836
KIBB7836.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 819 903 1155 1407 1836
Size: 819 84 252 252 429
Fragments arranged by size:
819 429 252 252 84
AflII C'TTAA_G
Cuts at: 0 1338 1836
Size: 1338 498
AflIII A'CryG_T
Cuts at: 0 652 1660 1836
Size: 652 1008 176
Fragments arranged by size:
1008 652 176
ApoI r'AATT_y
Cuts at: 0 417 484 591 597 1037 1121 1205 1373
Size: 417 67 107 6 440 84 84 168
Cuts at: 1373 1541 1709 1793 1836
Size: 168 168 84 43
Fragments arranged by size:
440 417 168 168 168 107 84 84
84 67 43 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 507 1836
Size: 507 1329
BfrBI ATG'CAT
Cuts at: 0 1461 1744 1836
Size: 1461 283 92
Fragments arranged by size:
1461 283 92
BmgBI CAC'GTC
Cuts at: 0 54 1836
Size: 54 1782
BmrI ACTGGGnnnn_n'
Cuts at: 0 166 1836
Size: 166 1670
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1202 1454 1836
Size: 1202 252 382
Fragments arranged by size:
1202 382 252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 731 1151 1319 1403 1487 1571 1739
Size: 151 580 420 168 84 84 84 168
Cuts at: 1739 1836
Size: 97
Fragments arranged by size:
580 420 168 168 151 97 84 84
84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 947 1836
Size: 947 889
BsaI GGTCTCn'nnnn_
Cuts at: 0 156 1836
Size: 156 1680
BsaHI Gr'CG_yC
Cuts at: 0 242 1836
Size: 242 1594
BsaWI w'CCGG_w
Cuts at: 0 1213 1836
Size: 1213 623
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 237 1836
Size: 237 1599
BseYI C'CCAG_C
Cuts at: 0 7 1836
Size: 7 1829
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1622 1836
Size: 1622 214
BsiHKAI G_wGCw'C
Cuts at: 0 65 268 1836
Size: 65 203 1568
Fragments arranged by size:
1568 203 65
BsmI GAATG_Cn'
Cuts at: 0 1463 1836
Size: 1463 373
BsmBI CGTCTCn'nnnn_
Cuts at: 0 249 1836
Size: 249 1587
Bsp1286I G_dGCh'C
Cuts at: 0 65 268 1836
Size: 65 203 1568
Fragments arranged by size:
1568 203 65
BspHI T'CATG_A
Cuts at: 0 1365 1836
Size: 1365 471
BsrBI CCG'CTC
Cuts at: 0 1438 1836
Size: 1438 398
BsrDI GCAATG_nn'
Cuts at: 0 1242 1836
Size: 1242 594
BstYI r'GATC_y
Cuts at: 0 210 367 1836
Size: 210 157 1469
Fragments arranged by size:
1469 210 157
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1505 1836
Size: 1505 331
ClaI AT'CG_AT
Cuts at: 0 1566 1836
Size: 1566 270
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1224 1259 1836
Size: 1224 35 577
Fragments arranged by size:
1224 577 35
DraI TTT'AAA
Cuts at: 0 726 743 1146 1650 1836
Size: 726 17 403 504 186
Fragments arranged by size:
726 504 403 186 17
EaeI y'GGCC_r
Cuts at: 0 489 1593 1836
Size: 489 1104 243
Fragments arranged by size:
1104 489 243
EarI CTCTTCn'nnn_
Cuts at: 0 377 1799 1836
Size: 377 1422 37
Fragments arranged by size:
1422 377 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 731 819 903 1151 1155 1319 1403
Size: 151 580 88 84 248 4 164 84
Cuts at: 1403 1407 1487 1571 1739 1836
Size: 4 80 84 168 97
Fragments arranged by size:
580 248 168 164 151 97 88 84
84 84 80 4 4
EcoO109I rG'GnC_Cy
Cuts at: 0 26 869 1836
Size: 26 843 967
Fragments arranged by size:
967 843 26
EcoRI G'AATT_C
Cuts at: 0 591 1121 1205 1373 1541 1709 1836
Size: 591 530 84 168 168 168 127
Fragments arranged by size:
591 530 168 168 168 127 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1077 1836
Size: 1077 759
HincII GTy'rAC
Cuts at: 0 287 1836
Size: 287 1549
HindIII A'AGCT_T
Cuts at: 0 746 1836
Size: 746 1090
MscI TGG'CCA
Cuts at: 0 491 1595 1836
Size: 491 1104 241
Fragments arranged by size:
1104 491 241
MslI CAynn'nnrTG
Cuts at: 0 476 558 1398 1509 1566 1836
Size: 476 82 840 111 57 270
Fragments arranged by size:
840 476 270 111 82 57
MspA1I CmG'CkG
Cuts at: 0 15 1836
Size: 15 1821
NsiI A_TGCA'T
Cuts at: 0 1463 1746 1836
Size: 1463 283 90
Fragments arranged by size:
1463 283 90
NspI r_CATG'y
Cuts at: 0 656 1664 1748 1834 1836
Size: 656 1008 84 86 2
Fragments arranged by size:
1008 656 86 84 2
PasI CC'CwG_GG
Cuts at: 0 215 1836
Size: 215 1621
PciI A'CATG_T
Cuts at: 0 652 1660 1836
Size: 652 1008 176
Fragments arranged by size:
1008 652 176
PflMI CCAn_nnn'nTGG
Cuts at: 0 879 1836
Size: 879 957
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 1836
Size: 136 32 1668
Fragments arranged by size:
1668 136 32
PpuMI rG'GwC_Cy
Cuts at: 0 869 1836
Size: 869 967
PsiI TTA'TAA
Cuts at: 0 1790 1836
Size: 1790 46
SexAI A'CCwGG_T
Cuts at: 0 146 1836
Size: 146 1690
SfcI C'TryA_G
Cuts at: 0 138 458 891 1227 1836
Size: 138 320 433 336 609
Fragments arranged by size:
609 433 336 320 138
SmlI C'TyrA_G
Cuts at: 0 962 1338 1836
Size: 962 376 498
Fragments arranged by size:
962 498 376
SwaI ATTT'AAAT
Cuts at: 0 726 1836
Size: 726 1110
TatI w'GTAC_w
Cuts at: 0 649 1836
Size: 649 1187
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 664 696 1836
Size: 664 32 1140
Fragments arranged by size:
1140 664 32
Tth111I GACn'n_nGTC
Cuts at: 0 282 1836
Size: 282 1554
XmnI GAAnn'nnTTC
Cuts at: 0 832 1205 1836
Size: 832 373 631
Fragments arranged by size:
832 631 373
Enzymes that do cut:
AcuI AflII AflIII ApoI BbsI BfrBI BmgBI BmrI
BplI BpmI BpuEI BsaI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrBI BsrDI
BstYI BtgZI ClaI CspCI DraI EaeI EarI Eco57MI
EcoO109I EcoRI FalI HincII HindIII MscI MslI MspA1I
NsiI NspI PasI PciI PflMI PpiI PpuMI PsiI
SexAI SfcI SmlI SwaI TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmtI Bpu10I BsaAI BsaBI BsaXI BsiEI BsiWI BspEI
BspMI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtsI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII
Hin4I HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PfoI PmeI PmlI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII XbaI
XcmI XhoI XmaI ZraI