Restriction map for
pF1KB7835
(Linear) (Six-base) MAPSORT of: KIBB7835.seq Check: 5750 from: 1 to: 1620
KIBB7835.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1586 1620
Size: 1586 34
AccI GT'mk_AC
Cuts at: 0 98 1224 1620
Size: 98 1126 396
Fragments arranged by size:
1126 396 98
Acc65I G'GTAC_C
Cuts at: 0 345 1620
Size: 345 1275
AflIII A'CryG_T
Cuts at: 0 122 1620
Size: 122 1498
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 254 1620
Size: 254 1366
ApaLI G'TGCA_C
Cuts at: 0 239 1620
Size: 239 1381
ApoI r'AATT_y
Cuts at: 0 449 946 1192 1620
Size: 449 497 246 428
Fragments arranged by size:
497 449 428 246
AseI AT'TA_AT
Cuts at: 0 742 1620
Size: 742 878
AvrII C'CTAG_G
Cuts at: 0 406 1620
Size: 406 1214
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 329 362 1620
Size: 329 33 1258
Fragments arranged by size:
1258 329 33
BamHI G'GATC_C
Cuts at: 0 1001 1620
Size: 1001 619
BanI G'GyrC_C
Cuts at: 0 345 1443 1620
Size: 345 1098 177
Fragments arranged by size:
1098 345 177
BanII G_rGCy'C
Cuts at: 0 768 1620
Size: 768 852
BbsI GAAGACnn'nnnn_
Cuts at: 0 131 1328 1620
Size: 131 1197 292
Fragments arranged by size:
1197 292 131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 526 560 1620
Size: 526 34 1060
Fragments arranged by size:
1060 526 34
BlpI GC'TnA_GC
Cuts at: 0 1607 1620
Size: 1607 13
Bme1580I G_kGCm'C
Cuts at: 0 243 1446 1620
Size: 243 1203 174
Fragments arranged by size:
1203 243 174
BmgBI CAC'GTC
Cuts at: 0 327 1620
Size: 327 1293
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 840 974 1539 1620
Size: 840 134 565 81
Fragments arranged by size:
840 565 134 81
Bpu10I CC'TnA_GC
Cuts at: 0 1532 1620
Size: 1532 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1025 1214 1620
Size: 1025 189 406
Fragments arranged by size:
1025 406 189
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1346 1376 1620
Size: 1346 30 244
Fragments arranged by size:
1346 244 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1370 1620
Size: 1370 250
BseYI C'CCAG_C
Cuts at: 0 690 1620
Size: 690 930
BsiHKAI G_wGCw'C
Cuts at: 0 243 768 1620
Size: 243 525 852
Fragments arranged by size:
852 525 243
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1352 1620
Size: 1352 268
Bsp1286I G_dGCh'C
Cuts at: 0 243 768 1446 1620
Size: 243 525 678 174
Fragments arranged by size:
678 525 243 174
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1586 1620
Size: 1586 34
BstEII G'GTnAC_C
Cuts at: 0 727 1620
Size: 727 893
BstYI r'GATC_y
Cuts at: 0 867 1001 1620
Size: 867 134 619
Fragments arranged by size:
867 619 134
BtsI GCAGTG_nn'
Cuts at: 0 1388 1620
Size: 1388 232
EaeI y'GGCC_r
Cuts at: 0 1308 1620
Size: 1308 312
EarI CTCTTCn'nnn_
Cuts at: 0 756 1336 1620
Size: 756 580 284
Fragments arranged by size:
756 580 284
EcoICRI GAG'CTC
Cuts at: 0 766 1620
Size: 766 854
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 840 974 1539 1620
Size: 840 134 565 81
Fragments arranged by size:
840 565 134 81
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1482 1620
Size: 1482 138
EcoRI G'AATT_C
Cuts at: 0 449 1620
Size: 449 1171
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 785 1620
Size: 785 835
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 859 880 891 912 1620
Size: 859 21 11 21 708
Fragments arranged by size:
859 708 21 21 11
HincII GTy'rAC
Cuts at: 0 99 1082 1225 1620
Size: 99 983 143 395
Fragments arranged by size:
983 395 143 99
HpaI GTT'AAC
Cuts at: 0 1082 1620
Size: 1082 538
KpnI G_GTAC'C
Cuts at: 0 349 1620
Size: 349 1271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 756 895 1620
Size: 756 139 725
Fragments arranged by size:
756 725 139
MspA1I CmG'CkG
Cuts at: 0 201 1620
Size: 201 1419
NspI r_CATG'y
Cuts at: 0 63 126 1620
Size: 63 63 1494
Fragments arranged by size:
1494 63 63
PasI CC'CwG_GG
Cuts at: 0 1205 1620
Size: 1205 415
PciI A'CATG_T
Cuts at: 0 122 1620
Size: 122 1498
PstI C_TGCA'G
Cuts at: 0 1383 1620
Size: 1383 237
PvuII CAG'CTG
Cuts at: 0 201 1620
Size: 201 1419
SacI G_AGCT'C
Cuts at: 0 768 1620
Size: 768 852
SalI G'TCGA_C
Cuts at: 0 97 1223 1620
Size: 97 1126 397
Fragments arranged by size:
1126 397 97
SapI GCTCTTCn'nnn_
Cuts at: 0 756 1620
Size: 756 864
ScaI AGT'ACT
Cuts at: 0 1261 1620
Size: 1261 359
SexAI A'CCwGG_T
Cuts at: 0 44 1620
Size: 44 1576
SfcI C'TryA_G
Cuts at: 0 129 1379 1620
Size: 129 1250 241
Fragments arranged by size:
1250 241 129
SmlI C'TyrA_G
Cuts at: 0 1040 1229 1620
Size: 1040 189 391
Fragments arranged by size:
1040 391 189
StuI AGG'CCT
Cuts at: 0 248 1157 1620
Size: 248 909 463
Fragments arranged by size:
909 463 248
StyI C'CwwG_G
Cuts at: 0 406 752 1620
Size: 406 346 868
Fragments arranged by size:
868 406 346
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 146 719 1620
Size: 146 573 901
Fragments arranged by size:
901 573 146
TatI w'GTAC_w
Cuts at: 0 125 931 1259 1620
Size: 125 806 328 361
Fragments arranged by size:
806 361 328 125
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1567 1599 1620
Size: 1567 32 21
Fragments arranged by size:
1567 32 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 430 1620
Size: 430 1190
XmnI GAAnn'nnTTC
Cuts at: 0 836 1325 1620
Size: 836 489 295
Fragments arranged by size:
836 489 295
Enzymes that do cut:
AarI AccI Acc65I AflIII AlfI ApaLI ApoI AseI
AvrII BaeI BamHI BanI BanII BbsI BcgI BlpI
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaXI BseRI BseYI
BsiHKAI BsmBI Bsp1286I BspMI BstEII BstYI BtsI EaeI
EarI EcoICRI Eco57MI EcoNI EcoRI FalI Hin4I HincII
HpaI KpnI MmeI MspA1I NspI PasI PciI PstI
PvuII SacI SalI SapI ScaI SexAI SfcI SmlI
StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AclI AcuI AfeI AflII AgeI AhdI AleI
AloI AlwNI ApaI AscI AsiSI AvaI BbeI BbvCI
BciVI BclI BfrBI BglI BglII BmrI BmtI BplI
BsaI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI
BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoO109I
EcoRV FseI FspI FspAI HaeII HindIII KasI MfeI
MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SanDI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI ZraI