Restriction map for
pF1KB7830
(Linear) (Six-base) MAPSORT of: KIBB7830.seq Check: 3233 from: 1 to: 1815
KIBB7830.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 486 903 983 1155 1407 1815
Size: 486 417 80 172 252 408
Fragments arranged by size:
486 417 408 252 172 80
AflII C'TTAA_G
Cuts at: 0 1338 1815
Size: 1338 477
AflIII A'CryG_T
Cuts at: 0 1660 1744 1815
Size: 1660 84 71
Fragments arranged by size:
1660 84 71
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1023 1815
Size: 1023 792
AlwNI CAG_nnn'CTG
Cuts at: 0 318 1815
Size: 318 1497
ApoI r'AATT_y
Cuts at: 0 594 953 1037 1121 1205 1373 1541 1709
Size: 594 359 84 84 84 168 168 168
Cuts at: 1709 1793 1815
Size: 84 22
Fragments arranged by size:
594 359 168 168 168 84 84 84
84 22
AvaI C'yCGr_G
Cuts at: 0 71 1815
Size: 71 1744
BanII G_rGCy'C
Cuts at: 0 164 1815
Size: 164 1651
BfrBI ATG'CAT
Cuts at: 0 1461 1815
Size: 1461 354
BmrI ACTGGGnnnn_n'
Cuts at: 0 166 1815
Size: 166 1649
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1202 1454 1815
Size: 1202 252 361
Fragments arranged by size:
1202 361 252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 815 1151 1319 1403 1487 1571 1739
Size: 151 664 336 168 84 84 84 168
Cuts at: 1739 1815
Size: 76
Fragments arranged by size:
664 336 168 168 151 84 84 84
76
BsaI GGTCTCn'nnnn_
Cuts at: 0 156 1815
Size: 156 1659
BsaAI yAC'GTr
Cuts at: 0 1693 1815
Size: 1693 122
BsaHI Gr'CG_yC
Cuts at: 0 242 1815
Size: 242 1573
BsaWI w'CCGG_w
Cuts at: 0 1213 1815
Size: 1213 602
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 146 176 1815
Size: 146 30 1639
Fragments arranged by size:
1639 146 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 237 1815
Size: 237 1578
BseYI C'CCAG_C
Cuts at: 0 7 1815
Size: 7 1808
BsmI GAATG_Cn'
Cuts at: 0 1463 1815
Size: 1463 352
BsmBI CGTCTCn'nnnn_
Cuts at: 0 249 1815
Size: 249 1566
Bsp1286I G_dGCh'C
Cuts at: 0 164 1815
Size: 164 1651
BsrDI GCAATG_nn'
Cuts at: 0 1242 1815
Size: 1242 573
BssSI C'ACGA_G
Cuts at: 0 1366 1815
Size: 1366 449
BstYI r'GATC_y
Cuts at: 0 210 331 1815
Size: 210 121 1484
Fragments arranged by size:
1484 210 121
ClaI AT'CG_AT
Cuts at: 0 1566 1815
Size: 1566 249
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1224 1259 1815
Size: 1224 35 556
Fragments arranged by size:
1224 556 35
DraI TTT'AAA
Cuts at: 0 1146 1650 1815
Size: 1146 504 165
Fragments arranged by size:
1146 504 165
EaeI y'GGCC_r
Cuts at: 0 1593 1815
Size: 1593 222
EarI CTCTTCn'nnn_
Cuts at: 0 256 309 1799 1815
Size: 256 53 1490 16
Fragments arranged by size:
1490 256 53 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 486 815 903 983 1151 1155 1319
Size: 151 335 329 88 80 168 4 164
Cuts at: 1319 1403 1407 1487 1571 1739 1815
Size: 84 4 80 84 168 76
Fragments arranged by size:
335 329 168 168 164 151 88 84
84 80 80 76 4 4
EcoO109I rG'GnC_Cy
Cuts at: 0 26 1809 1815
Size: 26 1783 6
Fragments arranged by size:
1783 26 6
EcoRI G'AATT_C
Cuts at: 0 594 1037 1121 1205 1373 1541 1709 1815
Size: 594 443 84 84 168 168 168 106
Fragments arranged by size:
594 443 168 168 168 106 84 84
EcoRV GAT'ATC
Cuts at: 0 1586 1815
Size: 1586 229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1077 1815
Size: 1077 738
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1271 1303 1815
Size: 1271 32 512
Fragments arranged by size:
1271 512 32
HincII GTy'rAC
Cuts at: 0 287 1815
Size: 287 1528
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 1815
Size: 359 1456
MscI TGG'CCA
Cuts at: 0 1595 1815
Size: 1595 220
MslI CAynn'nnrTG
Cuts at: 0 561 1398 1509 1566 1815
Size: 561 837 111 57 249
Fragments arranged by size:
837 561 249 111 57
MspA1I CmG'CkG
Cuts at: 0 15 1815
Size: 15 1800
NdeI CA'TA_TG
Cuts at: 0 811 1815
Size: 811 1004
NsiI A_TGCA'T
Cuts at: 0 1463 1815
Size: 1463 352
NspI r_CATG'y
Cuts at: 0 1664 1748 1815
Size: 1664 84 67
Fragments arranged by size:
1664 84 67
PasI CC'CwG_GG
Cuts at: 0 215 1815
Size: 215 1600
PciI A'CATG_T
Cuts at: 0 1660 1744 1815
Size: 1660 84 71
Fragments arranged by size:
1660 84 71
PflMI CCAn_nnn'nTGG
Cuts at: 0 879 1815
Size: 879 936
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 1815
Size: 136 32 1647
Fragments arranged by size:
1647 136 32
PpuMI rG'GwC_Cy
Cuts at: 0 1809 1815
Size: 1809 6
PsiI TTA'TAA
Cuts at: 0 857 1313 1790 1815
Size: 857 456 477 25
Fragments arranged by size:
857 477 456 25
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 723 755 1815
Size: 723 32 1060
Fragments arranged by size:
1060 723 32
SapI GCTCTTCn'nnn_
Cuts at: 0 256 1815
Size: 256 1559
SexAI A'CCwGG_T
Cuts at: 0 146 1815
Size: 146 1669
SfcI C'TryA_G
Cuts at: 0 138 461 891 1227 1815
Size: 138 323 430 336 588
Fragments arranged by size:
588 430 336 323 138
SmlI C'TyrA_G
Cuts at: 0 1338 1815
Size: 1338 477
StuI AGG'CCT
Cuts at: 0 935 1815
Size: 935 880
StyI C'CwwG_G
Cuts at: 0 349 1815
Size: 349 1466
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 457 1815
Size: 457 1358
TatI w'GTAC_w
Cuts at: 0 844 1815
Size: 844 971
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 146 178 1815
Size: 146 32 1637
Fragments arranged by size:
1637 146 32
XbaI T'CTAG_A
Cuts at: 0 1045 1815
Size: 1045 770
XmnI GAAnn'nnTTC
Cuts at: 0 1041 1205 1504 1756 1815
Size: 1041 164 299 252 59
Fragments arranged by size:
1041 299 252 164 59
Enzymes that do cut:
AcuI AflII AflIII AlfI AlwNI ApoI AvaI BanII
BfrBI BmrI BplI BpmI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsmI BsmBI Bsp1286I BsrDI BssSI
BstYI ClaI CspCI DraI EaeI EarI Eco57MI EcoO109I
EcoRI EcoRV FalI Hin4I HincII MmeI MscI MslI
MspA1I NdeI NsiI NspI PasI PciI PflMI PpiI
PpuMI PsiI PsrI SapI SexAI SfcI SmlI StuI
StyI TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmtI Bpu10I
BpuEI BsaBI BsgI BsiEI BsiHKAI BsiWI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI BtsI DraIII DrdI
EagI EciI EcoICRI EcoNI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NgoMIV NheI NotI NruI PacI PfoI PmeI
PmlI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XcmI XhoI XmaI ZraI