Restriction map for
pF1KB7824
(Linear) (Six-base) MAPSORT of: KIBB7824.seq Check: 8619 from: 1 to: 1812
KIBB7824.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 0 904 1547 1812
Size: 904 643 265
Fragments arranged by size:
904 643 265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 605 882 1134 1812
Size: 480 125 277 252 678
Fragments arranged by size:
678 480 277 252 125
AlwNI CAG_nnn'CTG
Cuts at: 0 628 1812
Size: 628 1184
ApoI r'AATT_y
Cuts at: 0 271 479 1812
Size: 271 208 1333
Fragments arranged by size:
1333 271 208
AvaI C'yCGr_G
Cuts at: 0 855 1812
Size: 855 957
BanI G'GyrC_C
Cuts at: 0 1259 1812
Size: 1259 553
BanII G_rGCy'C
Cuts at: 0 1102 1812
Size: 1102 710
BbsI GAAGACnn'nnnn_
Cuts at: 0 1800 1812
Size: 1800 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1216 1250 1812
Size: 1216 34 562
Fragments arranged by size:
1216 562 34
BglII A'GATC_T
Cuts at: 0 356 1812
Size: 356 1456
Bme1580I G_kGCm'C
Cuts at: 0 712 1229 1300 1384 1468 1812
Size: 712 517 71 84 84 344
Fragments arranged by size:
712 517 344 84 84 71
BmrI ACTGGGnnnn_n'
Cuts at: 0 511 667 1296 1812
Size: 511 156 629 516
Fragments arranged by size:
629 516 511 156
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 116 1812
Size: 116 1696
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 217 650 1072 1812
Size: 151 66 433 422 740
Fragments arranged by size:
740 433 422 151 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1153 1812
Size: 1153 659
BsaI GGTCTCn'nnnn_
Cuts at: 0 156 1812
Size: 156 1656
BsaAI yAC'GTr
Cuts at: 0 643 1545 1812
Size: 643 902 267
Fragments arranged by size:
902 643 267
BsaHI Gr'CG_yC
Cuts at: 0 807 1812
Size: 807 1005
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 26 853 883 1693 1723 1812
Size: 26 827 30 810 30 89
Fragments arranged by size:
827 810 89 30 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 238 310 1042 1075 1156 1492 1546 1549
Size: 238 72 732 33 81 336 54 3
Cuts at: 1549 1812
Size: 263
Fragments arranged by size:
732 336 263 238 81 72 54 33
3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 897 1812
Size: 897 915
BsiHKAI G_wGCw'C
Cuts at: 0 1102 1812
Size: 1102 710
BsmI GAATG_Cn'
Cuts at: 0 721 1812
Size: 721 1091
Bsp1286I G_dGCh'C
Cuts at: 0 712 1102 1229 1300 1384 1468 1812
Size: 712 390 127 71 84 84 344
Fragments arranged by size:
712 390 344 127 84 84 71
BsrGI T'GTAC_A
Cuts at: 0 113 1812
Size: 113 1699
BstEII G'GTnAC_C
Cuts at: 0 511 1329 1413 1812
Size: 511 818 84 399
Fragments arranged by size:
818 511 399 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 524 1812
Size: 524 1288
BstYI r'GATC_y
Cuts at: 0 356 1812
Size: 356 1456
BstZ17I GTA'TAC
Cuts at: 0 905 1812
Size: 905 907
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1649 1812
Size: 1649 163
DraIII CAC_nnn'GTG
Cuts at: 0 297 1812
Size: 297 1515
EaeI y'GGCC_r
Cuts at: 0 342 1572 1812
Size: 342 1230 240
Fragments arranged by size:
1230 342 240
EcoICRI GAG'CTC
Cuts at: 0 1100 1812
Size: 1100 712
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 217 480 605 650 882 1072 1134
Size: 151 66 263 125 45 232 190 62
Cuts at: 1134 1812
Size: 678
Fragments arranged by size:
678 263 232 190 151 125 66 62
45
EcoO109I rG'GnC_Cy
Cuts at: 0 107 445 950 1118 1812
Size: 107 338 505 168 694
Fragments arranged by size:
694 505 338 168 107
EcoRI G'AATT_C
Cuts at: 0 271 1812
Size: 271 1541
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1770 1812
Size: 1770 42
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 1701 1812
Size: 414 1287 111
Fragments arranged by size:
1287 414 111
MscI TGG'CCA
Cuts at: 0 344 1574 1812
Size: 344 1230 238
Fragments arranged by size:
1230 344 238
MslI CAynn'nnrTG
Cuts at: 0 509 789 1812
Size: 509 280 1023
Fragments arranged by size:
1023 509 280
MspA1I CmG'CkG
Cuts at: 0 195 1686 1812
Size: 195 1491 126
Fragments arranged by size:
1491 195 126
PasI CC'CwG_GG
Cuts at: 0 156 1812
Size: 156 1656
PflMI CCAn_nnn'nTGG
Cuts at: 0 297 1812
Size: 297 1515
PfoI T'CCnGG_A
Cuts at: 0 47 1812
Size: 47 1765
PpuMI rG'GwC_Cy
Cuts at: 0 107 1812
Size: 107 1705
PshAI GACnn'nnGTC
Cuts at: 0 248 1812
Size: 248 1564
PstI C_TGCA'G
Cuts at: 0 378 1720 1812
Size: 378 1342 92
Fragments arranged by size:
1342 378 92
PvuII CAG'CTG
Cuts at: 0 195 1812
Size: 195 1617
SacI G_AGCT'C
Cuts at: 0 1102 1812
Size: 1102 710
SexAI A'CCwGG_T
Cuts at: 0 293 1178 1812
Size: 293 885 634
Fragments arranged by size:
885 634 293
SfcI C'TryA_G
Cuts at: 0 138 374 651 1716 1812
Size: 138 236 277 1065 96
Fragments arranged by size:
1065 277 236 138 96
SmlI C'TyrA_G
Cuts at: 0 1132 1812
Size: 1132 680
SnaBI TAC'GTA
Cuts at: 0 1545 1812
Size: 1545 267
StuI AGG'CCT
Cuts at: 0 826 1812
Size: 826 986
StyI C'CwwG_G
Cuts at: 0 214 1812
Size: 214 1598
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 85 1812
Size: 85 1727
TatI w'GTAC_w
Cuts at: 0 113 1812
Size: 113 1699
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 28 344 376 1812
Size: 28 316 32 1436
Fragments arranged by size:
1436 316 32 28
Tth111I GACn'n_nGTC
Cuts at: 0 1776 1812
Size: 1776 36
XmnI GAAnn'nnTTC
Cuts at: 0 236 1036 1812
Size: 236 800 776
Fragments arranged by size:
800 776 236
Enzymes that do cut:
AccI AcuI AlwNI ApoI AvaI BanI BanII BbsI
BcgI BglII Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I
BsrGI BstEII BstXI BstYI BstZ17I BtgZI DraIII EaeI
EcoICRI Eco57MI EcoO109I EcoRI FalI MmeI MscI MslI
MspA1I PasI PflMI PfoI PpuMI PshAI PstI PvuII
SacI SexAI SfcI SmlI SnaBI StuI StyI TaqII
TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI
BfrBI BglI BlpI BmgBI BmtI Bpu10I BsaBI BsaWI
BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI Bsu36I BtgI
BtsI ClaI CspCI DraI DrdI EagI EarI EciI
EcoNI EcoRV FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SpeI SphI
SrfI SspI SwaI XbaI XcmI XhoI XmaI ZraI