(Linear) (Six-base) MAPSORT of: KIBB7821.seq  Check: 4226  from: 1  to: 1608



KIBB7821.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AccI GT'mk_AC

Cuts at:      0    1333    1608 
   Size:       1333     275
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     719    1541    1547    1608 
   Size:        719     822       6      61

  Fragments arranged by size: 

                 822     719      61       6
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     146    1608 
   Size:        146    1462
 
ApoI r'AATT_y

Cuts at:      0     140     268     359    1608 
   Size:        140     128      91    1249

  Fragments arranged by size: 

                1249     140     128      91
 
BanII G_rGCy'C

Cuts at:      0     621    1608 
   Size:        621     987
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1146    1608 
   Size:       1146     462
 
BbvCI CC'TCA_GC

Cuts at:      0      31    1608 
   Size:         31    1577
 
BglI GCCn_nnn'nGGC

Cuts at:      0      95    1608 
   Size:         95    1513
 
BglII A'GATC_T

Cuts at:      0    1161    1608 
   Size:       1161     447
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     778    1608 
   Size:        778     830
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     105    1528    1608 
   Size:        105    1423      80

  Fragments arranged by size: 

                1423     105      80
 
Bpu10I CC'TnA_GC

Cuts at:      0      31    1608 
   Size:         31    1577
 
BsaBI GATnn'nnATC

Cuts at:      0     668    1608 
   Size:        668     940
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1213    1556    1608 
   Size:       1213     343      52

  Fragments arranged by size: 

                1213     343      52
 
BsiHKAI G_wGCw'C

Cuts at:      0     399    1608 
   Size:        399    1209
 
BsmI GAATG_Cn'

Cuts at:      0    1354    1608 
   Size:       1354     254
 
Bsp1286I G_dGCh'C

Cuts at:      0     399     621    1608 
   Size:        399     222     987

  Fragments arranged by size: 

                 987     399     222
 
BsrFI r'CCGG_y

Cuts at:      0      21    1608 
   Size:         21    1587
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     502    1608 
   Size:        502    1106
 
BstBI TT'CG_AA

Cuts at:      0      13     525    1608 
   Size:         13     512    1083

  Fragments arranged by size: 

                1083     512      13
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     988    1608 
   Size:        988     620
 
BstYI r'GATC_y

Cuts at:      0    1014    1161    1608 
   Size:       1014     147     447

  Fragments arranged by size: 

                1014     447     147
 
BstZ17I GTA'TAC

Cuts at:      0    1334    1608 
   Size:       1334     274
 
BtsI GCAGTG_nn'

Cuts at:      0     951    1608 
   Size:        951     657
 
DraI TTT'AAA

Cuts at:      0     581     707    1608 
   Size:        581     126     901

  Fragments arranged by size: 

                 901     581     126
 
EaeI y'GGCC_r

Cuts at:      0      96    1608 
   Size:         96    1512
 
EarI CTCTTCn'nnn_

Cuts at:      0    1280    1608 
   Size:       1280     328
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     719    1528    1541    1547    1608 
   Size:        105     614     809      13       6      61

  Fragments arranged by size: 

                 809     614     105      61      13       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     472    1608 
   Size:        472    1136
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     160    1608 
   Size:        160    1448
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     448     480     799     831    1608 
   Size:        448      32     319      32     777

  Fragments arranged by size: 

                 777     448     319      32      32
 
HindIII A'AGCT_T

Cuts at:      0     177    1495    1608 
   Size:        177    1318     113

  Fragments arranged by size: 

                1318     177     113
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     290     851    1061    1608 
   Size:        290     561     210     547

  Fragments arranged by size: 

                 561     547     290     210
 
MscI TGG'CCA

Cuts at:      0      98    1608 
   Size:         98    1510
 
NaeI GCC'GGC

Cuts at:      0      23    1608 
   Size:         23    1585
 
NgoMIV G'CCGG_C

Cuts at:      0      21    1608 
   Size:         21    1587
 
NspI r_CATG'y

Cuts at:      0     505    1608 
   Size:        505    1103
 
PsiI TTA'TAA

Cuts at:      0     388    1608 
   Size:        388    1220
 
PstI C_TGCA'G

Cuts at:      0    1108    1608 
   Size:       1108     500
 
SfcI C'TryA_G

Cuts at:      0     263     831    1104    1427    1608 
   Size:        263     568     273     323     181

  Fragments arranged by size: 

                 568     323     273     263     181
 
SpeI A'CTAG_T

Cuts at:      0    1183    1608 
   Size:       1183     425
 
SphI G_CATG'C

Cuts at:      0     505    1608 
   Size:        505    1103
 
SspI AAT'ATT

Cuts at:      0     154    1608 
   Size:        154    1454
 
StuI AGG'CCT

Cuts at:      0     594    1608 
   Size:        594    1014
 
StyI C'CwwG_G

Cuts at:      0     712    1608 
   Size:        712     896
 
TatI w'GTAC_w

Cuts at:      0     219    1396    1608 
   Size:        219    1177     212

  Fragments arranged by size: 

                1177     219     212
 
XmnI GAAnn'nnTTC

Cuts at:      0     143    1608 
   Size:        143    1465
 

 Enzymes that do cut:

     AccI     AcuI     AlfI     ApoI    BanII     BbsI    BbvCI     BglI
    BglII     BmrI     BpmI   Bpu10I    BsaBI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BsrFI   BstAPI    BstBI    BstXI    BstYI  BstZ17I     BtsI
     DraI     EaeI     EarI  Eco57MI    EcoNI     FalI    Hin4I  HindIII
     MmeI     MscI     NaeI   NgoMIV     NspI     PsiI     PstI     SfcI
     SpeI     SphI     SspI     StuI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI
    BciVI     BclI    BfrBI     BlpI Bme1580I    BmgBI     BmtI     BplI
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstEII   Bsu36I     BtgI    BtgZI     ClaI
    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MslI   MspA1I     NarI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SrfI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI