Restriction map for
pF1KB7814
(Linear) (Six-base) MAPSORT of: KIBB7814.seq Check: 2143 from: 1 to: 1761
KIBB7814.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1629 1761
Size: 1629 132
AatII G_ACGT'C
Cuts at: 0 1327 1761
Size: 1327 434
AccI GT'mk_AC
Cuts at: 0 419 1004 1761
Size: 419 585 757
Fragments arranged by size:
757 585 419
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1132 1275 1761
Size: 1132 143 486
Fragments arranged by size:
1132 486 143
AflIII A'CryG_T
Cuts at: 0 692 1313 1761
Size: 692 621 448
Fragments arranged by size:
692 621 448
AlwNI CAG_nnn'CTG
Cuts at: 0 538 1761
Size: 538 1223
ApaI G_GGCC'C
Cuts at: 0 732 1761
Size: 732 1029
ApaLI G'TGCA_C
Cuts at: 0 700 1761
Size: 700 1061
AvaI C'yCGr_G
Cuts at: 0 614 1761
Size: 614 1147
BanI G'GyrC_C
Cuts at: 0 805 1459 1761
Size: 805 654 302
Fragments arranged by size:
805 654 302
BanII G_rGCy'C
Cuts at: 0 732 1761
Size: 732 1029
BbeI G_GCGC'C
Cuts at: 0 1463 1761
Size: 1463 298
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1547 1581 1761
Size: 1547 34 180
Fragments arranged by size:
1547 180 34
BglI GCCn_nnn'nGGC
Cuts at: 0 121 1761
Size: 121 1640
BglII A'GATC_T
Cuts at: 0 372 1761
Size: 372 1389
Bme1580I G_kGCm'C
Cuts at: 0 704 732 1761
Size: 704 28 1029
Fragments arranged by size:
1029 704 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 776 1761
Size: 776 985
BmtI G_CTAG'C
Cuts at: 0 1306 1761
Size: 1306 455
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 1761
Size: 319 1442
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 1761
Size: 559 1202
BsaAI yAC'GTr
Cuts at: 0 672 1761
Size: 672 1089
BsaHI Gr'CG_yC
Cuts at: 0 113 938 1280 1324 1460 1761
Size: 113 825 342 44 136 301
Fragments arranged by size:
825 342 301 136 113 44
BsaWI w'CCGG_w
Cuts at: 0 185 1761
Size: 185 1576
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 1500 1679 1682 1761
Size: 64 1436 179 3 79
Fragments arranged by size:
1436 179 79 64 3
BseYI C'CCAG_C
Cuts at: 0 57 1393 1406 1761
Size: 57 1336 13 355
Fragments arranged by size:
1336 355 57 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 501 1244 1686 1761
Size: 501 743 442 75
Fragments arranged by size:
743 501 442 75
BsiEI CG_ry'CG
Cuts at: 0 125 1761
Size: 125 1636
BsiHKAI G_wGCw'C
Cuts at: 0 704 990 1761
Size: 704 286 771
Fragments arranged by size:
771 704 286
BsmI GAATG_Cn'
Cuts at: 0 606 1154 1761
Size: 606 548 607
Fragments arranged by size:
607 606 548
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1315 1761
Size: 1315 446
Bsp1286I G_dGCh'C
Cuts at: 0 704 732 990 1761
Size: 704 28 258 771
Fragments arranged by size:
771 704 258 28
BspEI T'CCGG_A
Cuts at: 0 185 1761
Size: 185 1576
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1629 1761
Size: 1629 132
BsrBI CCG'CTC
Cuts at: 0 134 1186 1761
Size: 134 1052 575
Fragments arranged by size:
1052 575 134
BsrFI r'CCGG_y
Cuts at: 0 240 485 1761
Size: 240 245 1276
Fragments arranged by size:
1276 245 240
BsrGI T'GTAC_A
Cuts at: 0 344 1208 1761
Size: 344 864 553
Fragments arranged by size:
864 553 344
BssSI C'ACGA_G
Cuts at: 0 84 469 1761
Size: 84 385 1292
Fragments arranged by size:
1292 385 84
BstAPI GCAn_nnn'nTGC
Cuts at: 0 488 1761
Size: 488 1273
BstEII G'GTnAC_C
Cuts at: 0 1091 1569 1761
Size: 1091 478 192
Fragments arranged by size:
1091 478 192
BstYI r'GATC_y
Cuts at: 0 372 1235 1761
Size: 372 863 526
Fragments arranged by size:
863 526 372
BtgI C'CryG_G
Cuts at: 0 797 1463 1553 1667 1761
Size: 797 666 90 114 94
Fragments arranged by size:
797 666 114 94 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1179 1761
Size: 1179 582
BtsI GCAGTG_nn'
Cuts at: 0 1646 1761
Size: 1646 115
DraIII CAC_nnn'GTG
Cuts at: 0 210 761 1761
Size: 210 551 1000
Fragments arranged by size:
1000 551 210
EaeI y'GGCC_r
Cuts at: 0 122 666 677 1466 1761
Size: 122 544 11 789 295
Fragments arranged by size:
789 544 295 122 11
EagI C'GGCC_G
Cuts at: 0 122 1761
Size: 122 1639
EarI CTCTTCn'nnn_
Cuts at: 0 1569 1645 1761
Size: 1569 76 116
Fragments arranged by size:
1569 116 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1138 1204 1761
Size: 1138 66 557
Fragments arranged by size:
1138 557 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 1132 1275 1761
Size: 319 813 143 486
Fragments arranged by size:
813 486 319 143
EcoO109I rG'GnC_Cy
Cuts at: 0 1479 1761
Size: 1479 282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 278 1674 1761
Size: 278 1396 87
Fragments arranged by size:
1396 278 87
HaeII r_GCGC'y
Cuts at: 0 220 999 1207 1463 1658 1712 1761
Size: 220 779 208 256 195 54 49
Fragments arranged by size:
779 256 220 208 195 54 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1373 1405 1761
Size: 1373 32 356
Fragments arranged by size:
1373 356 32
HincII GTy'rAC
Cuts at: 0 657 1284 1311 1761
Size: 657 627 27 450
Fragments arranged by size:
657 627 450 27
KasI G'GCGC_C
Cuts at: 0 1459 1761
Size: 1459 302
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 1761
Size: 203 1558
MscI TGG'CCA
Cuts at: 0 1468 1761
Size: 1468 293
MslI CAynn'nnrTG
Cuts at: 0 697 1076 1270 1438 1761
Size: 697 379 194 168 323
Fragments arranged by size:
697 379 323 194 168
MspA1I CmG'CkG
Cuts at: 0 57 65 1761
Size: 57 8 1696
Fragments arranged by size:
1696 57 8
NarI GG'CG_CC
Cuts at: 0 1460 1761
Size: 1460 301
NcoI C'CATG_G
Cuts at: 0 1463 1553 1761
Size: 1463 90 208
Fragments arranged by size:
1463 208 90
NheI G'CTAG_C
Cuts at: 0 1302 1761
Size: 1302 459
NspI r_CATG'y
Cuts at: 0 696 1761
Size: 696 1065
PasI CC'CwG_GG
Cuts at: 0 1245 1761
Size: 1245 516
PciI A'CATG_T
Cuts at: 0 692 1761
Size: 692 1069
PflMI CCAn_nnn'nTGG
Cuts at: 0 675 1761
Size: 675 1086
PmlI CAC'GTG
Cuts at: 0 672 1761
Size: 672 1089
PpuMI rG'GwC_Cy
Cuts at: 0 1479 1761
Size: 1479 282
PshAI GACnn'nnGTC
Cuts at: 0 652 1761
Size: 652 1109
PspOMI G'GGCC_C
Cuts at: 0 728 1761
Size: 728 1033
SanDI GG'GwC_CC
Cuts at: 0 1479 1761
Size: 1479 282
SapI GCTCTTCn'nnn_
Cuts at: 0 1645 1761
Size: 1645 116
ScaI AGT'ACT
Cuts at: 0 1401 1761
Size: 1401 360
SexAI A'CCwGG_T
Cuts at: 0 1127 1761
Size: 1127 634
SfcI C'TryA_G
Cuts at: 0 1350 1587 1761
Size: 1350 237 174
Fragments arranged by size:
1350 237 174
SfoI GGC'GCC
Cuts at: 0 1461 1761
Size: 1461 300
SmlI C'TyrA_G
Cuts at: 0 574 614 1761
Size: 574 40 1147
Fragments arranged by size:
1147 574 40
SspI AAT'ATT
Cuts at: 0 21 1761
Size: 21 1740
StyI C'CwwG_G
Cuts at: 0 1097 1463 1553 1761
Size: 1097 366 90 208
Fragments arranged by size:
1097 366 208 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1557 1761
Size: 1557 204
TatI w'GTAC_w
Cuts at: 0 344 1208 1399 1761
Size: 344 864 191 362
Fragments arranged by size:
864 362 344 191
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 694 726 1761
Size: 694 32 1035
Fragments arranged by size:
1035 694 32
Tth111I GACn'n_nGTC
Cuts at: 0 1342 1761
Size: 1342 419
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1551 1761
Size: 1551 210
XhoI C'TCGA_G
Cuts at: 0 614 1761
Size: 614 1147
ZraI GAC'GTC
Cuts at: 0 1325 1761
Size: 1325 436
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BcgI BglI BglII Bme1580I
BmrI BmtI BpmI BpuEI BsaAI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstEII BstYI
BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI
Eco57MI EcoO109I FalI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NarI NcoI NheI NspI PasI
PciI PflMI PmlI PpuMI PshAI PspOMI SanDI SapI
ScaI SexAI SfcI SfoI SmlI SspI StyI TaqII
TatI TstI Tth111I XcmI XhoI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BciVI BclI BfrBI BlpI BmgBI BplI
Bpu10I BsaI BsaBI BsaXI BsiWI BspHI BsrDI BssHII
BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraI DrdI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NotI
NruI NsiI PacI PfoI PmeI PpiI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI XbaI XmaI XmnI